Microbial ecology of drinking water from source to tap

The Science of The Total Environment, Journal Year: 2023, Volume and Issue: 908, P. 168077 - 168077

Published: Oct. 31, 2023

Language: Английский

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Improved random forest classification model combined with C5.0 algorithm for vegetation feature analysis in non-agricultural environments

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: May 6, 2024

Abstract In response to the challenges posed by high computational complexity and suboptimal classification performance of traditional random forest algorithms when dealing with high-dimensional noisy non-agricultural vegetation satellite data, this paper proposes an enhanced algorithm based on C5.0 algorithm. The focuses Liaohe Plain, selecting two distinct landscape patterns in Shenbei New District Changtu County as research objects. High-resolution data from GF-2 serves experimental dataset. This introduces ensemble feature method bagging concept improve original model. enhances likelihood features beneficial classifying positive class samples, avoiding excessive removal useful negative samples. approach ensures importance model diversity. is then employed for selection, index (EVI) utilized coverage estimation. Results indicate that employing a multi-scale parameter selection tool, combined limited field-measured facilitates identification plant species landscapes. effectively selects features, minimizing information redundancy. established object-oriented achieves impressive accuracy 94.02% aerial imagery dataset, EVI-based estimation demonstrating accuracy. experiments same test set, proposed attains average 90.20%, outperforming common such bidirectional encoder representation transformer, FastText, convolutional neural network, which achieve accuracies ranging 84.41 88.33% identifying artificial habitat features. exhibits competitive edge compared other algorithms. These findings contribute scientific evidence protecting agricultural ecosystems restoring ecosystem biodiversity.

Language: Английский

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An outbreak of Legionnaires’ disease linked to a municipal and industrial wastewater treatment plant, The Netherlands, September–October 2022

Eurosurveillance, Journal Year: 2024, Volume and Issue: 29(20)

Published: May 16, 2024

Wastewater treatment plants (WWTPs) are increasingly identified as Legionnaires’ disease (LD) sources. An outbreak investigation was initiated following five LD cases reported in September 2022 Houten, the Netherlands. Case identification based on European case definition, with symptom onset from 1 2022, residence or within 5 km of visit to Houten incubation period, without other likely We sampled potential sources and genotyped environmental clinical isolates. 15 between 13 23 October 2022. A spatial source wind direction model suggested an industrial (iWWTP) a municipal WWTP (mWWTP) sources, first discharging water into latter. Both tested positive for Legionella pneumophila serogroups 6 multiple sequence types (ST). detected L. sg1 ST42 mWWTP, matching one three available Following control measures at WWTPs, no further were observed. This underlines that WWTPs can play important role community outbreaks, especially those favourable conditions growth dissemination, even non-favourable but influx contaminated water.

Language: Английский

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Legionella pneumophila subverts the antioxidant defenses of its amoeba host Acanthamoeba castellanii.

Current Research in Microbial Sciences, Journal Year: 2025, Volume and Issue: 8, P. 100338 - 100338

Published: Jan. 1, 2025

Legionella pneumophila, the causative agent of Legionnaires' disease, interacts in environment with free-living amoebae that serve as replicative niches for bacteria. Among these amoebae, Acanthamoeba castellanii is a natural host water networks and model commonly used to study interaction between L. pneumophila its host. However, certain crucial aspects this remain unclear. One such aspect role oxidative stress, studies focusing on reactive oxygen species (ROS) production by putting less emphasis involvement host's antioxidant defenses during infectious process. In study, we propose examine consequences infection wild-type or an isogenic ΔdotA mutant strain, which unable replicate intracellularly, A. castellanii. For purpose, looked at ROS levels, defense transcripts, metabolites linked amoeba's defenses. It known WT can block activation NADPH oxidase soon it enters macrophage suppress compared strain. addition, has been shown macrophages decreases 24 h p.i.; here confirm result suggest decrease could be partly explained differentially regulated transcripts 6 p.i.. We also explored metabolome infected not pneumophila. 617 identified, four reduced abundances may involved responses. This suggests hijack replication maintain level ROS.

Language: Английский

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Between green and safety: navigating sustainability and public health risks in growing media

International Journal of Vegetable Science, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 7

Published: Jan. 15, 2025

Production and use of growing media involve a trade-off between sustainability public health. We claim that paradigm shift is needed, focusing on properties supporting environmental human health, with holistic approach involving One Health expertise. Legionella in media, soil, gardening as an example to highlight key knowledge gaps.

Language: Английский

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Legionella pneumophila response to shifts in biofilm structure mediated by hydrodynamics

Biofilm, Journal Year: 2025, Volume and Issue: unknown, P. 100258 - 100258

Published: Jan. 1, 2025

Language: Английский

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An Innovative Device for the Hot Water Circuit in Hospitals to Save Energy Without Compromising the Safety and Quality of Water: Preliminary Results

Water, Journal Year: 2025, Volume and Issue: 17(5), P. 692 - 692

Published: Feb. 27, 2025

Legionella colonization of water systems represents a potential hazard for humans within healthcare facilities. It is possible to contain its spread through continuous disinfection and the correct management maintenance systems. The hygienic sanitary quality cannot be ignored in an evaluation energy costs. Fondazione Policlinico Universitario A. Gemelli IRCCS Rome has installed “ME.SI. MR ACS” (MEthod SavIngs Maximum eneRgy hot water) device, which allows system activate, when necessary, avoiding recirculation. objectives this study are evaluate health hygiene hospital network thermal electrical savings chlorine dioxide consumption, with without device operation. This involved three phases microbiological sampling facility under study: ME.SI. ACS but not running, boilers’ setpoint temperature at 60 °C; running operation 45 °C. analyses were carried out accordance ISO standard. data show constant absence spp. all samples. application on recirculation circuit leads reduction daily consumption 68.6% 48.6%, respectively, approximately EUR 23,000/year per circuit. Furthermore, operation, there 50% 11,500/year. guarantees associated maintaining network.

Language: Английский

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Detection, relatedness and environmental sources of emerging Legionella longbeachae infections in Finland, 1989-2024

Diagnostic Microbiology and Infectious Disease, Journal Year: 2025, Volume and Issue: 112(2), P. 116788 - 116788

Published: March 6, 2025

Legionella longbeachae is an emerging cause of Legionnaires' disease in Europe. We analysed data from the National Infectious Disease Register (NIDR) and Reference Laboratory database to characterize L. infections diagnostics Finland. A total 41 patient cases, mainly gardeners' pneumonias hand wounds, were reported during 1989-2024, with a significant increase after 2019. Patient interviews environmental sampling linked compost, potting soil or outdoor activities. Whole genome sequencing (WGS) confirmed identical isolates three cases revealed several clusters among non-related isolates. Compared caused by other species, had distinct characteristics, including higher median age (64 vs. 56 yrs.), fatality (15 % 8 %), more extrapulmonary (12 1 %) proportion females (44 35 %). The genetic relatedness high clonality suggest common reservoirs.

Language: Английский

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Appraisal of Microbial Indoor Air Quality in Applied Medical Sciences Collage

Salud Ciencia y Tecnología, Journal Year: 2025, Volume and Issue: 5, P. 1152 - 1152

Published: Feb. 24, 2025

Introduction: Human activities like talking, sneezing, coughing, walking, washing, and toilet use contribute to an increased airborne microbiological load. The air is full of various microorganisms, which act as a medium for their transmission or dissemination. ‎This study aimed determine the types concentrations bacterial fungal aerosols, evaluate indoor quality, factors responsible presence in College Applied Medical Sciences building, PSAU, KSA. Methods: Indoor microbial loads were evaluated by collecting 84 samples from different localities using settle plate method. Results: average ranges 0 150.7 13.1 242.5 CFU per m3 fungi bacteria, respectively. In indoor-to-outdoor ratio, results recorded 0.033 0.067 0.022 0.049 A total 282 bacteria identified, 2 isolates belonging Gram-positive cocci (Kocuria rhizophila 3.3%, Staphylococcus epidermidis 15%), (14%), rod Bacillus spp. (39%). One isolate was identified Sphingomonas paucimobilis (0.7%). Fungal (n=48) isolated; 46 filamentous 9(18.8%) Aspergillus (A. niger, A. terreus, ochraceus, other spp.), Alternaria sp. 8(16.7%) Penicillium spp., 3(6.3%) Fusarium 2(4.2%) Rhizopus Cladosporium 1(2.1%) Drechslera sp., 12(25%) unknown species, addition two yeast isolates. Conclusions: building safe suitable current number students, building's design same condition.

Language: Английский

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Concurrent contamination of Free-Living Amoebae and Legionella pneumophila in water resources of hospitals, A microscopic and molecular analysis

Research Square (Research Square), Journal Year: 2025, Volume and Issue: unknown

Published: April 2, 2025

Free-living amoebae (FLAs) are widely prevalent protozoa found in various natural and man-made environments. In Iran, there have been few studies examining the contamination of hospital environments with FLAs Legionella spp., none explored coexistence these two organisms. Thus, this study aims to investigate spp. water resources hospitals Birjand city. Water samples were collected from sources different departments city, transferred laboratory. One litre each sample passed through nitrocellulose membrane filter. Samples taken taps storage tanks positive analyzed by morphological keys Polymerase chain reaction (PCR) using 18S rDNA gene ITS region identify amoeba isolates. The possible was examined cultures molecular tests. Overall, 80 out 270 for culture method. Acanthamoeba isolated all that Acanthamoeba. Among which parasite grown culture, 64 isolates also All sequenced reported as T4 genotype. Furthermore, 5/270 L. pneumophila contamination. cases infection infected Acantamoeba. A significant difference observed between concurrent Acantamoeba Razi (P_value=001) Imam Reza (P_value= 004) well surgical wards 0002) 0.01) at hospitals. This aimed examine co-occurrence free-living most important water-related pathogens, systems better understand their interactions potential consequents public health, can lead both outbreaks sporadic cases.

Language: Английский

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