Subchronic oral exposure to polystyrene microplastics affects hepatic lipid metabolism, inflammation, and oxidative balance in gilthead seabream (Sparus aurata)

Ecotoxicology and Environmental Safety, Journal Year: 2024, Volume and Issue: 279, P. 116455 - 116455

Published: May 20, 2024

Microplastics (MPs) pose a clear threat to aquatic organisms affecting their health. Their impact on liver homeostasis, as well the potential onset of nonalcoholic fatty disease (NAFLD), is still poorly investigated and remains almost unknown. The aim this study was evaluate outcomes subchronic exposure polystyrene MPs (PS-MPs; 1–20 μm; 0, 25, or 250 mg/kg b.w./day) lipid metabolism, inflammation, oxidative balance in gilthead seabreams (Sparus aurata Linnaeus, 1758) exposed for 21 days via contaminated food. PS-MPs induced an up-regulation mRNA levels crucial genes associated with synthesis storage (i.e., PPARy, Srebp1, Fasn) without modifications involved catabolism PPARα, HL, Pla2) transport metabolism (Fabp1) liver. increase CSF1R pro-inflammatory cytokines gene expression TNF-α IL-1β) also observed fish dose-dependent manner. These findings were confirmed by hepatic histological evaluations reporting evidence accumulation, necrosis. Moreover, caused impairment antioxidant defense system through alteration its enzymatic (catalase, superoxide dismutase, glutathione reductase) non-enzymatic (glutathione) components, resulting increased production reactive oxygen species (ROS) malondialdehyde (MDA), biomarkers damage. detoxifying enzymes inferred decreased Ethoxyresorufin-O-deethylase (EROD) activity glutathione-S-transferase (GST) at highest PS-MP dose. suggests that affect health seabream. dysfunction damage result from detrimental interplay damage, systems modifications, altering gut-liver axis homeostasis. This scenario suggestive involvement MP-induced effects progression

Language: Английский

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Bio inspired heuristic computing scheme for the human liver nonlinear model

Heliyon, Journal Year: 2024, Volume and Issue: 10(7), P. e28912 - e28912

Published: April 1, 2024

In this research, a bio-inspired heuristic computing approach has been developed to solve the nonlinear behavior of human liver, which is categorized into liver and blood. The solutions model are presented by using stochastic computation procedure based on artificial neural network (ANN) along with optimization genetic algorithm (GA) interior-point (IP). A fitness function designed through differential form then optimized hybrid competency GAIP scheme. correctness exactness proposed observed overlapping obtained (GAIP) reference (Adams scheme) solutions, while calculated absolute error values in good order enhance worth solver. log-sigmoid transfer together ten numbers neurons executed perform model. Furthermore, statistical approaches have applied observe reliability for solving

Language: Английский

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Cyhalofop-butyl induced hepatotoxicity of zebrafish through disturbing the gut-liver axis

Microchemical Journal, Journal Year: 2025, Volume and Issue: unknown, P. 112912 - 112912

Published: Jan. 1, 2025

Language: Английский

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Assessing the effects of a commercial fungicide and an herbicide, alone and in combination, on Apis mellifera: Insights from biomarkers and cognitive analysis

Chemosphere, Journal Year: 2024, Volume and Issue: 359, P. 142307 - 142307

Published: May 9, 2024

Agrochemicals play a vital role in protecting crops and enhancing agricultural production by reducing threats from pests, pathogens weeds. The toxicological status of honey bees can be influenced number factors, including pesticides. While extensive research has focused on the lethal sublethal effects insecticides individual colonies, it is important to recognise that fungicides herbicides also affect bee health. Unfortunately, field, are exposed mixtures compounds rather than single substances. This study aimed evaluate commercial fungicide herbicide, both individually combination, bees. Mortality assays, biomarkers learning memory tests were performed, results integrated assess Neurotoxicity (acetylcholinesterase carboxylesterase activities), detoxification metabolic processes (glutathione S-transferase alkaline phosphatase immune system function (lysozyme activity haemocyte count) genotoxicity (nuclear abnormalities assay) assessed. Sakura® was found activate enzymes activity. herbicide Elegant 2FD combination pesticides showed neurotoxic induced processes. Exposure herbicide/fungicide mixture impaired represents significant advance understanding commonly used agriculture contributes development effective strategies mitigate their adverse non-target insects.

Language: Английский

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“Cocaine Shark”: First report on cocaine and benzoylecgonine detection in sharks

The Science of The Total Environment, Journal Year: 2024, Volume and Issue: 948, P. 174798 - 174798

Published: July 15, 2024

Language: Английский

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Implications of ammonia stress for the pathogenicity of Shewanella spp. in Oreochromis niloticus: effects on hematological, biochemical, immunological, and histopathological parameters

BMC Veterinary Research, Journal Year: 2024, Volume and Issue: 20(1)

Published: July 18, 2024

Abstract Environmental stressors (such as ammonia) in aquaculture could increase the risk of pathogenicity, posing a more severe threat to farmed fish. The aim this study was investigate effects ammonia stress on pathogenicity Shewanella spp. Oreochromis niloticus . First, 96-hour static test used determine median lethal concentration (LC 50 ) unionized Nile tilapia. After 96 h exposure, Un-ionized (UIA) LC estimated be 4.26 mg/L. Second, an experiment conducted effect O. for 30 days. A involved 180 fish divided into six groups, with first group serving control. second (AMN1/10) and third (AMN1/20) were not challenged exposed 1/10 (0.42 mg/L) 1/20 (0.21 UIA, respectively. Then 0.2 mL (0.14 × 10 5 intraperitoneally injected fourth (SH), fifth (SH + AMN1/10), sixth AMN1/20) which subjected 0, mg/L), survival rate, hematological indices, immunological parameters, antioxidant activity significantly decreased when they infection separately or together. Histopathological changes also observed kidney liver. Furthermore, both individual combined exposures altered renal hepatic function, notable increases glucose cortisol levels, well expression proinflammatory cytokine genes ( TNF-α IL-1ß ). However, detrimental co-exposure greater than those separate exposures. As result, we may say that increased concentrations enhance These findings contribute better understanding

Language: Английский

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NUTRITIONAL STUDIES ON FISH CATLA CATLA FOUND IN GANGA RIVER WITH HYDROBIOLOGICAL STUDIES

Journal of Science Innovations and Nature of Earth, Journal Year: 2024, Volume and Issue: 4(2), P. 17 - 22

Published: June 22, 2024

Finding out what other people have said and thought about a certain subject then putting that information into writing is literature review all about. One way to get more knowledgeable in your area do review. Fundamental ideas, research strategies, experimental procedures utilised profession will be covered. Discover real-world challenges. further advantage of reading reviews you gain better grasp the presentation discussion findings specific field. Improving skills for field as simple paying close attention others written attempting adopt their style. The purpose reviewing nutritional studies on fish Catla catla found Ganga river with hydrobiological understand effects environmental factors, especially heavy metal pollution, quality health. an important food feeds plankton occupies upper layer water column. It widely distributed India South Asian countries. However, facing severe threats from various anthropogenic activities such industrial effluents, mining waste, agricultural runoff, domestic sewage. These sources introduce metals nickel thallium water, which can build up tissues, leading histopathological alterations, immunotoxicity, genotoxicity, oxidative stress. As result, keeping eye samples levels crucial, determining fish's profile terms calories, ash, protein, carbohydrates, lipids. To assess how contamination affects diversity quality, it necessary compare data places times year. Insights gained this also help ecosystem management resource conservation.Even if isn't required class or major project, it's still good idea so you're up-to-date discussions surrounding choice. become equipped construct argument uniquely yours.

Language: Английский

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Method for assessing the content of molybdenum enzymes in the internal organs of fish

MethodsX, Journal Year: 2024, Volume and Issue: 12, P. 102576 - 102576

Published: Jan. 19, 2024

Molybdenum enzymes (Mo-enzymes) contain a molybdenum cofactor (MoCo) in the active site. These are potentially interesting for studying survival mechanism of fish under hypoxic water conditions. This is because Mo-enzymes can synthesize nitric oxide from nitrates and nitrites, which present high concentrations However, there currently no method assessing content internal organs. Methods capable determining major importance. For this purpose, quantitative determination MoCo plant tissues was modified. We demonstrated Mo-enzyme assessment by isolated fish's organs Neurospora crassa nit-1 extract containing inactive NADPH nitrate reductase. The Mo enzyme calculated using calibration curve nM nitrites as product restored reductase activity after complementation with MoCo. Here we robust laboratory be used to assess fish. play crucial role detoxifying toxic compounds. Therefore, it important develop accurately determine amount present. Notably, efficiency accuracy detected liver intestines (P <0.0001). obtained data on distribution species correspond that other vertebrates. Here, rapid, sensitive, accurate accessible method. developed simple easy use. Importantly, protocol does not require complex manipulations, equipment available most laboratories. article provides step-by-step instructions reproducing

Language: Английский

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Behavioral, biochemical, immune, and histological responses of Nile tilapia (Oreochromis niloticus Linnaeus, 1758) to lead, mercury, and pendimethalin exposure: individual and combined effects

Environmental Sciences Europe, Journal Year: 2025, Volume and Issue: 37(1)

Published: Jan. 15, 2025

Abstract The aquaculture sector is facing environmental issues because of rising chemical pollution aquatic systems, which may be caused by man-made or natural causes. combined action these mixtures can produce an effect that either severalfold higher lower than the overall toxicity individual components. objective this study to evaluate impact three toxicants: lead (Pb 0.088 mg L −1 ), mercury (Hg 0.084 and pendimethalin (PM 0.355 ) their combinations on behavior, oxidative stress, immunology, biochemistry, histopathological changes in Nile tilapia. results show mortality was evident across all treatment groups highest mixture (MIX) group, at 33.4%. Some behavioral included increased mucus secretion, fin rot, dark skin coloration fish, were evidently exacerbated MIX group. erythrocyte hemoglobin indices presented marked reductions PM comparison control group had levels serum creatinine urea, as well liver enzymes alanine aminotransferase, aspartate transaminase, alkaline phosphatase. They also malondialdehyde total antioxidant capacity, showed stress. stress biomarkers, cortisol glucose, greater groups. neurobehavioral assessment demonstrated noticeable decreased acetylcholinesterase MIX-exposed In immunological parameters, nitric oxide lysozyme lower, especially experimental groups, spleen pro-inflammatory cytokine genes higher. examination revealed lesions gills, liver, brains form inflammation, necrosis, circulatory alterations. Among exposed exhibited changes. Collectively, our findings significant toxic effects heavy metals herbicides tilapia, revealing a synergistic both physiological responses resulting from exposures. data indicate ecological hazards associated with ecosystems.

Language: Английский

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An improved ultrasound-assisted extraction (UAE) and HPLC-PDA methods for multiclass analysis of pharmaceutical residues in fish

Journal of Liquid Chromatography &amp Related Technologies, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 8

Published: Feb. 12, 2025

Language: Английский

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