Development of a gradual hypoxia chamber for assessing copper toxicity on air-breathing behavior in Lymnaea stagnalis

Toxicology Mechanisms and Methods, Journal Year: 2025, Volume and Issue: 35(4), P. 422 - 429

Published: Jan. 7, 2025

Behavioral endpoints are of increasing interest in toxicology because their sensitivity, but require clear guidance for experimental design. This study describes the design a hypoxia chamber use with pond snails, Lymnaea stagnalis. Studies assessing switch from water- to air-breathing hypoxic conditions have previously utilized methods that neglect intricacies animal behavior such as handling stress and acclimation. The provides linear decline dissolved oxygen, against which surfacing can be precisely measured. maximum biomass snails suitable chamber, nitrogen-driven deoxygenation curve is not altered by snails' own metabolism, was established greater than 10 adult snails. capacity most analysis softwares below accurately tracking individuals at once, indicating this likely limitation. size determined amount time each episode aerial respiration was, smaller spending more air-breathing. A proof-of-principle experiment using acute copper exposure (0 – 60 µg/L) yielded concentration-response curve, concentrations inhibiting described present an improved framework response presented manner allowing further modification meet unique research needs.

Language: Английский

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Behavioral alterations in adult zebrafish induced by venlafaxine: correlation with oxidative stress, gene expression, and brain histopathological damage.

Environmental Research, Journal Year: 2025, Volume and Issue: unknown, P. 121550 - 121550

Published: April 1, 2025

Language: Английский

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Effect of an antidepressant on aquatic ecosystems in the presence of microplastics: A mesocosm study

Environmental Pollution, Journal Year: 2024, Volume and Issue: 357, P. 124439 - 124439

Published: June 26, 2024

Emerging pollutants, such as pharmaceuticals and microplastics have become a pressing concern due to their widespread presence potential impacts on ecological systems. To assess the ecosystem-level effects of these pollutants within multi-stressor context, we simulated real-world conditions by exposing near-natural multi-trophic aquatic food web gradient environmentally relevant concentrations fluoxetine in large mesocosms over period more than three months. We measured biomass abundance different trophic groups, well functions nutrient availability decomposition rate. explore mechanisms underlying community effects, also performed behavioral assays focusing locomotion parameters response variable species: Daphnia magna (zooplankton prey), Chaoborus flavicans larvae (invertebrate pelagic predator zooplankton) Asellus aquaticus (benthic macroinvertebrate), using water from mesocosms. Our mesocosm results demonstrate that governs phytoplankton biomass, with weak non-monotonic dose-response relationship interaction between fluoxetine. However, exposure evoked strong zooplankton microbial rate plant material. In assays, prey D. showed similar primarily induced Its C. flavicans, however, significant governed both The behavior decomposer A. significantly decreased at higher concentrations, potentially leading reduced rates near sediment. study demonstrates observed upon short-term result pronounced following chronic exposure.

Language: Английский

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Quantification and risk assessment of polar organic contaminants in two chalk streams in Hampshire, UK using the Chemcatcher passive sampler

The Science of The Total Environment, Journal Year: 2024, Volume and Issue: 939, P. 173316 - 173316

Published: May 21, 2024

Freshwater systems are facing a number of pressures due to the inputs polar organic contaminants from range sources including agriculture, domestic and industry. The River Itchen Test two sensitive chalk streams in Southern England that experiencing decline invertebrate communities. We used Chemcatcher passive samplers measure time-weighted average concentrations (14 days) pollutants at nine sites on eight over 12-month period. Sampler extracts were analysed using targeted LC/MS method. In total, 121 plant protection products pharmaceutical personal care quantified (range log K

Language: Английский

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Quantification and Risk Assessment of Polar Organic Contaminants in Two Chalk Streams in Hampshire, UK Using the Chemcatcher Passive Sampler

Published: Jan. 1, 2024

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Language: Английский

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