The effects of concentration, duration of exposure, size and surface function of polymethyl methacrylate micro/nanoplastics on human liver cells DOI Creative Commons

Weilin L. Shelver,

Lloyd O. Billey,

Amy M. McGarvey

et al.

Ecotoxicology and Environmental Safety, Journal Year: 2024, Volume and Issue: 287, P. 117240 - 117240

Published: Oct. 31, 2024

Micro/nanoplastics (MP/NP) are pervasive contaminants that detected throughout the environment in diverse matrices. Exposure to MP/NP have been demonstrated humans by their presence numerous body fluids and tissues. Due large quantity of production broad applications, polymethyl methacrylate (PMMA) frequently measured surveys microplastics environment. The effects size, surface charge (aminated, carboxylated or non-functionalized), concentration, exposure duration PMMA particles HepG2 human liver cells were evaluated this study. majority non-cytotoxic. Some sporadic cytotoxicity was but it did not follow discernable trends. Confocal images revealed 50, 100, 1000 nm all taken up irrespective charge. Particle size significantly affected caspase-3 release (p = 0.0002). Apoptosis induced only a small number at 24 48 h for 50 MP/NP. From 72 - 120 h, apoptosis increased time dependent manner beads 100 µg/mL three functionalizations, with amine having highest (36 %). Production pro-inflammatory interleukin-8 (IL-8) > 2x when from 4 particle charge, concentration. Collectively, cytotoxic concentrations tested, able translocate into cells, caspase-3, induce apoptosis, produce IL-8 fashion.

Language: Английский

An updated systematic review about various effects of microplastics on cancer: A pharmacological and in-silico based analysis DOI
Akmaral Baspakova, Afshin Zare,

Roza Suleimenova

et al.

Molecular Aspects of Medicine, Journal Year: 2025, Volume and Issue: 101, P. 101336 - 101336

Published: Jan. 4, 2025

Language: Английский

Citations

0
Studying mercury and polymethyl methacrylate joint effects on endogenous hydrogen polysulfides via fluorescence imaging DOI
Li Zhang, Liangwei Zhang, Yang Yang

et al.

Journal of Hazardous Materials, Journal Year: 2025, Volume and Issue: 489, P. 137643 - 137643

Published: Feb. 17, 2025

Language: Английский

Citations

0
Biological Effects of Micro-/Nano-Plastics in Macrophages DOI Creative Commons
Massimiliano G. Bianchi, Lavinia Casati,

Giulia Sauro

et al.

Nanomaterials, Journal Year: 2025, Volume and Issue: 15(5), P. 394 - 394

Published: March 4, 2025

The environmental impact of plastics is worsened by their inadequate end-of-life disposal, leading to the ubiquitous presence micro- (MPs) and nanosized (NPs) plastic particles. MPs NPs are thus widely present in water air inevitably enter food chain, with inhalation ingestion as main exposure routes for humans. Many recent studies have demonstrated that gain access several body compartments, where they taken up cells, increase production reactive oxygen species, lead inflammatory changes. In most tissues, resident macrophages engage first approach foreign materials, this interaction largely affects subsequent fate material possible pathological outcomes. On other hand, organizers controllers both responses tissue repair. Here, we aim summarize available information on NPs. Particular attention will be devoted consequences macrophage viability functions, well implications pathology.

Language: Английский

Citations

0
Distribution and Risk Assessment of Microplastics in the Basin of the Pearl River, China DOI

Fengxue Wu,

Jingfu Wang,

Shihao Jiang

et al.

Water Air & Soil Pollution, Journal Year: 2025, Volume and Issue: 236(5)

Published: April 10, 2025

Language: Английский

Citations

0
Filler effect on properties of polymethyl methacrylate-based membranes for hemodialysis application DOI
Adel Ouradi, Nabila Cherifi,

Yasmina Afir

et al.

Journal of Polymer Research, Journal Year: 2025, Volume and Issue: 32(4)

Published: April 1, 2025

Language: Английский

Citations

0
Systematic Comparison of Commercial Uranyl‐Alternative Stains for Negative‐ and Positive‐Staining Transmission Electron Microscopy of Organic Specimens DOI Creative Commons
Vera M. Kissling,

Stephanie Eitner,

Davide Bottone

et al.

Advanced Healthcare Materials, Journal Year: 2025, Volume and Issue: unknown

Published: April 29, 2025

Abstract Negative‐ and positive‐staining transmission electron microscopy (ns/psTEM) is a cornerstone of research diagnostics, enabling nanometer‐resolution analysis organic specimens from nanoparticles to cells without requiring costly cryo‐equipment. For nearly 70 years, uranyl salts like acetate (UA) have been the gold‐standard ns/psTEM‐stains. However, mounting safety concerns due their high toxicity radioactivity led stricter regulations expensive licensing requirements. Consequently, there an urgent global demand for safer, more sustainable stains that deliver uranyl‐comparable, high‐quality ns/psTEM. Here, commercially available stain‐alternatives UranyLess, UAR, UA‐Zero, PTA, STAIN 77, Nano‐W, NanoVan, lead citrate are systematically assessed against UA. The evaluated regarding contrast, resolution, stain‐distribution, ease‐of‐use in ns/psTEM across diverse sample set, including polymethylmethacrylate‐nanoplastics, phosphatidylcholine‐liposomes, Influenza‐A viruses, globular ferritin, fibrillar pyruvate kinase amyloids, human lung‐carcinoma cell‐sections. It shown this variety samples, ready‐to‐use uranyl‐alternative with comparable or even superior ns/psTEM‐performance UA using efficient staining‐protocol. Furthermore, GUIDE4U tool developed fast identification appropriate uranyl‐replacements each interest, saving ns/psTEM‐users time costs while ensuring excellent staining results ultrastructural analysis, thereby further catalyzing use safer stains.

Language: Английский

Citations

0
Polystyrene Microplastics‐Induced Thyroid Dysfunction in Mice: A Study of Gene Expression, Oxidative Stress, and Histopathological Changes DOI Creative Commons
Md. Sadequl Islam, Md. Kamruzzaman, UK Rima

et al.

Veterinary Medicine and Science, Journal Year: 2025, Volume and Issue: 11(3)

Published: May 1, 2025

ABSTRACT Background Polystyrene microplastics (PS‐MPs) are pervasive pollutants impacting animals across ecosystems, including livestock and wildlife, through contaminated food, water, air. MPs may disrupt endocrine function, particularly affecting the thyroid gland, which is essential for metabolism development. Objectives This study investigates effects of PS‐MPs on function in mice, offering insights relevant to veterinary care by examining changes gene expression biochemical markers. Methods 5 µm diameter were prepared distilled water after probe sonication. Sixty male Swiss albino mice divided into three groups: a control group two treatment groups receiving 0.1 mg 0.2 via oral gavage 28 days. Mice anesthetised, tissues collected histopathological, biochemical, analyses. Biochemical tests included catalase, superoxide dismutase, reactive oxygen species, hormone levels. Histopathology ( TSHR TPO ) thyroid‐related genes examined assess induced effects. Results Exposure led significant increases calcium, thyroxin, free T3, T4, ALP, AST, ALT, amylase levels, alongside elevated oxidative stress Conversely, levels TSH, calcitonin, magnesium phosphate decreased. Histopathological analysis showed abnormal follicle development, decrease parafollicular cells, with colloid loss, haemorrhage, necrosis. Gene revealed marked reduction treated groups, indicating dysfunction. These findings highlight profound impact gland mice. Conclusion underscore potential risks that pose health, consequences other species. As environmental contamination rises, veterinarians encounter more disorders linked PS‐MPs, emphasising need further research preventive measures.

Language: Английский

Citations

0
The unseen perils of oral-care products generated micro/nanoplastics on human health DOI Creative Commons

Utsa Saha,

Snehasmita Jena,

Faizan Zarreen Simnani

et al.

Ecotoxicology and Environmental Safety, Journal Year: 2024, Volume and Issue: 290, P. 117526 - 117526

Published: Dec. 13, 2024

Language: Английский

Citations

2
The effects of concentration, duration of exposure, size and surface function of polymethyl methacrylate micro/nanoplastics on human liver cells DOI Creative Commons

Weilin L. Shelver,

Lloyd O. Billey,

Amy M. McGarvey

et al.

Ecotoxicology and Environmental Safety, Journal Year: 2024, Volume and Issue: 287, P. 117240 - 117240

Published: Oct. 31, 2024

Micro/nanoplastics (MP/NP) are pervasive contaminants that detected throughout the environment in diverse matrices. Exposure to MP/NP have been demonstrated humans by their presence numerous body fluids and tissues. Due large quantity of production broad applications, polymethyl methacrylate (PMMA) frequently measured surveys microplastics environment. The effects size, surface charge (aminated, carboxylated or non-functionalized), concentration, exposure duration PMMA particles HepG2 human liver cells were evaluated this study. majority non-cytotoxic. Some sporadic cytotoxicity was but it did not follow discernable trends. Confocal images revealed 50, 100, 1000 nm all taken up irrespective charge. Particle size significantly affected caspase-3 release (p = 0.0002). Apoptosis induced only a small number at 24 48 h for 50 MP/NP. From 72 - 120 h, apoptosis increased time dependent manner beads 100 µg/mL three functionalizations, with amine having highest (36 %). Production pro-inflammatory interleukin-8 (IL-8) > 2x when from 4 particle charge, concentration. Collectively, cytotoxic concentrations tested, able translocate into cells, caspase-3, induce apoptosis, produce IL-8 fashion.

Language: Английский

Citations

0