Evaluating the Impact of Environmental Factors on Bacterial Populations in Riverine, Estuarine, and Coastal Sediments DOI Creative Commons
Ramganesh Selvarajan, Ming Yang, Henry Joseph Oduor Ogola

et al.

Diversity, Journal Year: 2024, Volume and Issue: 16(12), P. 749 - 749

Published: Dec. 6, 2024

Aquatic ecosystems, including rivers, estuaries, and coastal environments, are crucial for maintaining biodiversity, regulating nutrient cycles, supporting human livelihoods. However, these ecosystems increasingly being threatened by urbanization, making it essential to understand their microbial communities ecological roles. This study employed high-throughput 16S rRNA gene sequencing characterize the bacterial within riverine, estuarine, sediments of Adyar Creek, Chennai, India. Proteobacteria were dominant phylum across most samples, with proportions ranging from 39.65% 72.09%. Notably, estuarine environment exhibited a distinct taxonomic profile characterized significant abundance Firmicutes (47.09% population). Distinct classes observed sediment types: Alphaproteobacteria (30.07–34.32%) in riverine sediments, Bacilli dominated (40.17%), Gammaproteobacteria (15.71–51.94%) sediments. The environmental factors influencing community composition samples pH, salinity, phosphate, nitrate. LEfSe (Linear discriminant analysis Effect Size) identified specific genera estuary, Bacillus (20.26%), unclassified_Paenibacillus (12.87%), Clostridium (3.81%), Gailella (3.17%), Paenibacillus (3.02%), Massilia (1.70%), Paraburkholderia (1.42%), Pantoea (1.15%), as potential biomarkers habitat health. Functional revealed an elevated expression genes associated ABC transporters carbon metabolism suggesting heightened cycling capacity. Furthermore, co-occurrence network indicated that exhibit strong modular structure complex species interactions three types. These findings highlight communities’ critical role key drivers establishing baseline further investigations into functional ecology vulnerable ecosystems.

Language: Английский

Exploring biodegradation limits of n-alkanes as polyethylene models using multi-omics approaches DOI Creative Commons
Miwa Suzuki,

Takuya Hayashi,

Kohei Takahashi

et al.

The Science of The Total Environment, Journal Year: 2025, Volume and Issue: 977, P. 179365 - 179365

Published: April 14, 2025

Polyethylene (PE) is widely regarded as non-biodegradable in natural environments, despite reports suggesting partial biotic degradation. Using multi-omics analysis, this study investigated the biodegradation mechanisms of n-alkanes-structural analogs PE-to determine threshold carbon number PE that allows for environmental biodegradation. n-Alkanes with 6-40 carbons (C6-C40) were biodegraded soil, whereas C44 and not. 16S rRNA gene amplicon sequence analysis identified distinct microbial communities associated non-degradable compounds (PEs C44) biodegradable alkanes (C6-C40). Notably, community composition C40 differed from those below C36. Multi-omics genera Aeromicrobium, Nocardia, Nocardioides, Rhodococcus, Acinetobacter, Fontimonas key degraders n-alkanes at C36 below, utilizing alkane hydroxylases such monooxygenase (AlkB), LC-alkane Acinetobacter (AlmA), cytochrome P450 (CYP153). Conversely, was facilitated by taxa, including order Acidimicrobiales genera, Acidovorax, Sphingorhabdus, Prosthecobacter, Roseimicrobium using AlmA CYP153-type hydroxylases. This difference may explain reduced biodegradability above C40, PE.

Language: Английский

Citations

0
Functional and Taxonomic Insights into Root Endophytic Bacterial Communities in Hybrid Tomato and Finger Millet: Implications for Crop Resilience and Growth DOI Creative Commons

Kannaiah Surendirakumar,

Ramganesh Selvarajan,

Salam Nirmalashachi Chanu

et al.

Microbiology Research, Journal Year: 2025, Volume and Issue: 16(3), P. 61 - 61

Published: March 6, 2025

Root endophytic microbial communities play a key role in plant health and productivity, yet the extent to which these vary across different crop species remains underexplored. This study aimed investigate root bacterial diversity of two important crops—hybrid tomato (Lycopersicon esculentum Mill. var. TNAU CO3) finger millet (Eleusine coracana L. CO13)—to understand how crop-specific microbiomes contribute agricultural sustainability productivity. Targeted 16S rDNA amplicon sequencing was performed on samples. A total 165,772 181,327 rRNA gene sequences were generated from roots, respectively. These processed identify sequence variants (ASVs), then classified taxonomically assess community composition functional prediction. Across hybrid crops, 1400 ASVs detected 1838 millet. Proteobacteria (52.61–62.09%) dominant phylum both, followed by Actinobacteria, Firmicutes, Bacteroidota, unidentified bacteria, Myxococcota, Verrucomicrobiota, Acidobacteriota, Fusobacteriota, Chloroflexi. Finger roots harbored more diverse robust assemblage, particularly enriched with nitrogen-fixing oxidative stress-mitigating bacteria. In contrast, showed higher abundance phosphate-solubilizing biofilm-forming taxa, potentially enhancing resilience environmental stress. findings highlight nature their capabilities. By revealing distinct profiles millet, this work provides valuable foundation for developing strategies optimize soil health, performance, abiotic stress tolerance.

Language: Английский

Citations

0
Genome-resolved analysis of Serratia marcescens SMTT infers niche specialization as a hydrocarbon-degrader DOI Creative Commons
Amanda C. Ramdass, Sephra N. Rampersad

DNA Research, Journal Year: 2024, Volume and Issue: 32(1)

Published: Dec. 27, 2024

Abstract Bacteria that are chronically exposed to high levels of pollutants demonstrate genomic and corresponding metabolic diversity complement their strategies for adaptation hydrocarbon-rich environments. Whole genome sequencing was carried out infer functional traits Serratia marcescens strain SMTT recovered from soil contaminated with crude oil. The size (Mb) 5,013,981 a total gene count 4,842. Comparative analyses carefully selected S. strains, 2 which associated soil, show conservation central pathways in addition intra-specific genetic flexibility. Genome comparisons also indicated an enrichment genes multidrug resistance efflux pumps SMTT. contained enable the catabolism aromatic compounds via protocatechuate para-degradation pathway, meta-cleavage catechol (meta-cleavage pathway II); compound degradation markedly higher compared other strains analysed. Our data presents valuable inventory future studies on provides insights into those features industrial potential.

Language: Английский

Citations

1
Evaluating the Impact of Environmental Factors on Bacterial Populations in Riverine, Estuarine, and Coastal Sediments DOI Creative Commons
Ramganesh Selvarajan, Ming Yang, Henry Joseph Oduor Ogola

et al.

Diversity, Journal Year: 2024, Volume and Issue: 16(12), P. 749 - 749

Published: Dec. 6, 2024

Aquatic ecosystems, including rivers, estuaries, and coastal environments, are crucial for maintaining biodiversity, regulating nutrient cycles, supporting human livelihoods. However, these ecosystems increasingly being threatened by urbanization, making it essential to understand their microbial communities ecological roles. This study employed high-throughput 16S rRNA gene sequencing characterize the bacterial within riverine, estuarine, sediments of Adyar Creek, Chennai, India. Proteobacteria were dominant phylum across most samples, with proportions ranging from 39.65% 72.09%. Notably, estuarine environment exhibited a distinct taxonomic profile characterized significant abundance Firmicutes (47.09% population). Distinct classes observed sediment types: Alphaproteobacteria (30.07–34.32%) in riverine sediments, Bacilli dominated (40.17%), Gammaproteobacteria (15.71–51.94%) sediments. The environmental factors influencing community composition samples pH, salinity, phosphate, nitrate. LEfSe (Linear discriminant analysis Effect Size) identified specific genera estuary, Bacillus (20.26%), unclassified_Paenibacillus (12.87%), Clostridium (3.81%), Gailella (3.17%), Paenibacillus (3.02%), Massilia (1.70%), Paraburkholderia (1.42%), Pantoea (1.15%), as potential biomarkers habitat health. Functional revealed an elevated expression genes associated ABC transporters carbon metabolism suggesting heightened cycling capacity. Furthermore, co-occurrence network indicated that exhibit strong modular structure complex species interactions three types. These findings highlight communities’ critical role key drivers establishing baseline further investigations into functional ecology vulnerable ecosystems.

Language: Английский

Citations

0