Characterization of Glyphosate Resistance and Degradation Profile of Caballeronia zhejiangensis CEIB S4-3 and Genes Involved in Its Degradation

Microorganisms, Journal Year: 2025, Volume and Issue: 13(3), P. 651 - 651

Published: March 13, 2025

Herbicides are the most employed pesticides in agriculture worldwide; among them, glyphosate is successful herbicide molecule history. The extensive use of has been related to environmental pollution and toxic effects on non-target organisms. Effective remediation treatment alternatives must be developed reduce presence its adverse effects. Bioremediation using microorganisms proposed as a feasible alternative for treating pollution; due this, identifying characterizing capable biodegrading key task bioremediation polluted sites by this herbicide. This study characterized resistance profile degradation capacity bacterial strain Caballeronia zhejiangensis CEIB S4-3. According results growth inhibition assays agar plates, C. S4-3 can resist exposure high concentrations glyphosate, up 1600 mg/L glyphosate-based (GBH) formulation, 12,000 analytical-grade molecule. In assay liquid media, resisted all evaluated (25–400 mg/L). After 48 h exposure, GBH caused important (>80%) at between 100 400 mg/L, while inhibitions below 15% tested concentrations. Finally, was degrading 60% supplemented culture media (50 mg/L), when used sole carbon source, twelve hours; moreover, also degrade primary metabolite aminomethylphosphonic acid (AMPA). Genomic analysis revealed genes associated with two reported metabolic pathways degradation, sarcosine AMPA pathways. first report metabolism genus strain. from investigation demonstrate that exhibits significant potential biodegradation, suggesting applicability strategies targeting contaminant.

Language: Английский

Microbial degradation of contaminants of emerging concern: metabolic, genetic and omics insights for enhanced bioremediation

Frontiers in Bioengineering and Biotechnology, Journal Year: 2024, Volume and Issue: 12

Published: Sept. 19, 2024

The perpetual release of natural/synthetic pollutants into the environment poses major risks to ecological balance and human health. Amongst these, contaminants emerging concern (CECs) are characterized by their recent introduction/detection in various niches, thereby causing significant hazards necessitating removal. Pharmaceuticals, plasticizers, cyanotoxins pesticides groups CECs that highly toxic found occur compartments biosphere. sources these compounds can be multipartite including industrial discharge, improper disposal, excretion unmetabolized residues, eutrophication etc ., while fate persistence determined factors such as physico-chemical properties, environmental conditions, biodegradability hydrological factors. resultant exposure microbiota has imposed a selection pressure resulted evolution metabolic pathways for biotransformation and/or utilization sole source carbon energy. Such microbial degradation phenotype exploited clean-up from environment, offering cost-effective eco-friendly alternative abiotic methods removal, mitigating toxicity. However, efficient bioprocess development bioremediation strategies requires extensive understanding individual components pathway gene clusters, proteins/enzymes, metabolites associated regulatory mechanisms. “Omics” “Meta-omics” techniques aid providing crucial insights complex interactions functions well community, enabling more effective targeted bioremediation. Aside natural isolates, engineering approaches employ application genetic enhance diversity rates. integration omics data will further developing systemic-level strategies, optimising process. This review describes bacterial catabolic pathways, genetics, four CECs: pharmaceuticals, cyanotoxins, pesticides.

Language: Английский