Transcriptomic and Biochemical analysis of Procambarus clarkii upon exposure to Pesticides: Population-Specific responses as a sign of pollutant resistance?
Environmental Research,
Journal Year:
2025,
Volume and Issue:
unknown, P. 120963 - 120963
Published: Jan. 1, 2025
Language: Английский
A comparative evaluation of haematological and biochemical parameters of Nemipterus randalli and Pagellus erythrinus species living in Gökova Bay, Türkiye
PeerJ,
Journal Year:
2025,
Volume and Issue:
13, P. e18784 - e18784
Published: Jan. 20, 2025
This
study
aims
to
determine
and
compare
the
reference
values
of
haematological
biochemical
blood
parameters
two
fish
species
collected
from
Gökova
Bay
(Muğla,
South-Western
Türkiye):
non-native
invasive
Randall’s
threadfin
bream,
Nemipterus
randalli
native
Common
pandora,
Pagellus
erythrinus
.
Both
inhabit
same
environment
compete
for
resources.
Blood
samples
were
a
total
100
(50
N.
50
P.
)
which
caught
depth
30
60
meters
between
February
2023
July
2024.
Therefore,
sampling
could
be
carried
out
in
summer
winter
seasons
when
water
temperature
varies
significantly.
The
results
indicate
that
red
cell
(RBC),
haemoglobin
(Hb)
haematocrit
(Hct)
levels
significantly
increases
(
p
<
0.05).
Statistical
analyses
revealed
all
are
higher
than
In
terms
serum
parameters,
glucose
(GLU),
triglyceride
(TRIG),
cholesterol
(CHOL),
protein
(TP),
aspartate
aminotransferase
(AST)
alkaline
phosphatase
(ALP)
had
showed
no
significant
difference
both
>
However,
alanine
(ALT)
statistically
different
compared
Additionally,
highest
lactate
dehydrogenase
(LDH)
value
was
measured
this
found
other
groups
Multidimensional
scaling
(MDS)
analysis
differences
parameter
individuals.
provides
important
data
about
physiological
adaptations
environmental
factors.
similarity
possibility
distribute
new
habitats
where
is
distributed.
suggests
further
potential
distribution
need
careful
monitoring.
Language: Английский
Transcriptomic and Biochemical analysis ofProcambarus clarkiiupon exposure to Pesticides: Population-Specific responses as a sign of pollutant resistance?
bioRxiv (Cold Spring Harbor Laboratory),
Journal Year:
2024,
Volume and Issue:
unknown
Published: Oct. 8, 2024
Abstract
The
effects
that
anthropogenic
stressors
may
have
on
modulating
species
plasticity
has
been
relatively
unexplored;
however,
it
represents
a
scientific
frontier
offer
insights
into
their
ability
to
colonize
new
habitats.
To
explore
the
advantage
inhabiting
polluted
environments
invasive
species,
we
selected
crayfish
Procambarus
clarkii
,
can
and
thrive
in
wide
range
of
aquatic
environments,
including
heavily
ones.
Here,
studied
molecular
physiological
responses
when
experimentally
exposed
pesticide
mix
azoxystrobin
oxadiazon
at
sublethal
concentrations.
We
compared
these
three
isolated
populations
Southern
France
are
established
areas
with
different
pollution
levels:
a)
Camargue,
seasonally
affected
by
pollution;
b)
Bages-Sigean,
impacted
all
year-round
domestic
effluents
and;
c)
Salagou,
more
pristine
site.
Gene
expression
analyses
revealed
response
was
strongest
Camargue
crayfish.
In
this
population,
total
88
differentially
expressed
genes
(DEGs)
were
identified
hepatopancreas
78
gills
between
control
laboratory
groups.
Among
successfully
annotated,
those
involved
stress,
DNA
repair,
immune
response,
translation
transcription
processes
stand
out.
Interestingly,
responded
mainly
upregulation,
but
downregulation
gills.
This
suggests
naïve
individuals,
biocides
natural
habitat
respond
mechanistic
strategies
confer
them
adaptability
population
level.
Responses
terms
antioxidant
detoxification
enzymes
also
corroborate
differences
biocide
inputs
according
origin
Language: Английский