Human dermal fibroblast senescence in response to single and recurring oxidative stress DOI Creative Commons

Tailynn Y. McCarty,

Cathal J. Kearney

Frontiers in Aging, Journal Year: 2025, Volume and Issue: 6

Published: March 28, 2025

Introduction: Aging results in an accumulation of damaged cells, which reduces the health tissues and their regenerative capabilities. In skin, there are both internal external drivers oxidative stress that result aging phenotypes. Oxidative has been used to model senescence vitro; however, a lack research determining whether severity correlates with senescent Methods: this work, we compare cellular secretory responses single (500 μM hydrogen peroxide, 2 hours) or recurring dose peroxide hours + 4 × 300 each 48 hours). Senescence induction was studied using markers including cell morphology, senescence-associated-beta-galactosidase, absence apoptosis, cycle inhibition genes. Next, functional studies effects signaling these cells were completed, such as vascular potential, keratinocyte proliferation, macrophage polarization. Results: Fibroblasts exposed had increased total nucleic area, senescence-associated-beta-galactosidase (SABGAL) expression, they able escape apoptosis - all characteristics cells. Additionally, expressed levels inhibitor genes decreased expression collagen-I, -III, -IV. Cytokine profiling showed stressed more inflammatory profile. However, assays, reduced IL-1β gene unpolarized polarized macrophages. Discussion: The described protocol allows for investigation direct fibroblasts on surrounding healthy These show recurringly represent intense phenotype, can be vitro understand responses.

Language: Английский

Human dermal fibroblast senescence in response to single and recurring oxidative stress DOI Creative Commons

Tailynn Y. McCarty,

Cathal J. Kearney

Frontiers in Aging, Journal Year: 2025, Volume and Issue: 6

Published: March 28, 2025

Introduction: Aging results in an accumulation of damaged cells, which reduces the health tissues and their regenerative capabilities. In skin, there are both internal external drivers oxidative stress that result aging phenotypes. Oxidative has been used to model senescence vitro; however, a lack research determining whether severity correlates with senescent Methods: this work, we compare cellular secretory responses single (500 μM hydrogen peroxide, 2 hours) or recurring dose peroxide hours + 4 × 300 each 48 hours). Senescence induction was studied using markers including cell morphology, senescence-associated-beta-galactosidase, absence apoptosis, cycle inhibition genes. Next, functional studies effects signaling these cells were completed, such as vascular potential, keratinocyte proliferation, macrophage polarization. Results: Fibroblasts exposed had increased total nucleic area, senescence-associated-beta-galactosidase (SABGAL) expression, they able escape apoptosis - all characteristics cells. Additionally, expressed levels inhibitor genes decreased expression collagen-I, -III, -IV. Cytokine profiling showed stressed more inflammatory profile. However, assays, reduced IL-1β gene unpolarized polarized macrophages. Discussion: The described protocol allows for investigation direct fibroblasts on surrounding healthy These show recurringly represent intense phenotype, can be vitro understand responses.

Language: Английский

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