Mechanisms of Ellagic Acid (EA)-Mediated Osteogenic Differentiation of Human Dental Pulp-Derived Stem Cells DOI Creative Commons

Prathyusha Naidu,

Manjusri Das,

Surajit Hansda

et al.

ACS Omega, Journal Year: 2025, Volume and Issue: 10(15), P. 15229 - 15242

Published: April 13, 2025

Ellagic acid (EA) is a potent antioxidant that reduces oxidative stress and promotes differentiation. By lowering the harmful levels of reactive oxygen species (ROS), EA fosters an environment conducive to osteoblastic differentiation (OB) stem cells. In addition, it autophagy mitophagy, which are vital for promoting Effective autophagic activity recycles damaged organelles proteins, meeting energy required during shielding from apoptosis. However, molecular mechanisms underlying osteogenic mesenchymal cells remain inadequately explored. Therefore, current study aims define regulatory role OB dental pulp-derived (DPSC) how mitophagy being modulated this process. Herein, we showed expression level osteoblast-specific markers, autophagy, mitophagy-associated markers was significantly elevated EA-mediated DPSC. Moreover, found induced osteoblastic-specific through canonical BMP2 pathway molecules, reduced ROS in both basal activated states, molecules along with enhanced mitochondrial functions. Cell cycle analysis revealed G1 phase arrested via phosphorylation γ-H2AX, ATM, CHK2 proteins. Furthermore, silico strongly binds osteonectin, crucial noncollagen protein involved bone remodeling, confirmed by Western blot analysis. These results support could be promising natural compound repair regeneration applications.

Language: Английский

Mechanisms of Ellagic Acid (EA)-Mediated Osteogenic Differentiation of Human Dental Pulp-Derived Stem Cells DOI Creative Commons

Prathyusha Naidu,

Manjusri Das,

Surajit Hansda

et al.

ACS Omega, Journal Year: 2025, Volume and Issue: 10(15), P. 15229 - 15242

Published: April 13, 2025

Ellagic acid (EA) is a potent antioxidant that reduces oxidative stress and promotes differentiation. By lowering the harmful levels of reactive oxygen species (ROS), EA fosters an environment conducive to osteoblastic differentiation (OB) stem cells. In addition, it autophagy mitophagy, which are vital for promoting Effective autophagic activity recycles damaged organelles proteins, meeting energy required during shielding from apoptosis. However, molecular mechanisms underlying osteogenic mesenchymal cells remain inadequately explored. Therefore, current study aims define regulatory role OB dental pulp-derived (DPSC) how mitophagy being modulated this process. Herein, we showed expression level osteoblast-specific markers, autophagy, mitophagy-associated markers was significantly elevated EA-mediated DPSC. Moreover, found induced osteoblastic-specific through canonical BMP2 pathway molecules, reduced ROS in both basal activated states, molecules along with enhanced mitochondrial functions. Cell cycle analysis revealed G1 phase arrested via phosphorylation γ-H2AX, ATM, CHK2 proteins. Furthermore, silico strongly binds osteonectin, crucial noncollagen protein involved bone remodeling, confirmed by Western blot analysis. These results support could be promising natural compound repair regeneration applications.

Language: Английский

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