RNA Methyltransferase NSUN5 Promotes Esophageal Cancer via 5‐Methylcytosine Modification of METTL1 DOI Open Access

YuanBo Cui,

Zhaoyang Hu, Chunyan Zhang

et al.

Molecular Carcinogenesis, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 27, 2024

Aberrant RNA modifications can drive carcinogenic transformation and tumor progression, with 5-methylcytosine (m5C) emerging as one of the predominant in eukaryotic cells. However, function molecular mechanisms m5C esophageal cancer (ESCA) remain insufficiently defined. Here we report that methyltransferase NOP2/Sun domain family member 5 (NSUN5) is significantly upregulated ESCA tumors shows promising diagnostic potential. Functionally, knockdown NSUN5 impairs proliferation capacity cells arrests cell cycle at G0/G1 phase, while enforced expression accelerates progression. In vivo, deficiency reduces growth a cell-based xenograft mouse model. Mechanistically, correlates oncogenic like 1 (METTL1), positively regulating its expression; binds directly to METTL1 transcript, facilitating modification Additionally, overexpression effectively counteracts tumor-suppressive effects resulting from ablation both vitro vivo settings. A comprehensive pan-cancer analysis further underscores NSUN5's essential role digestive system tumors, downregulation notably inhibiting gastric colon growth. These findings provide new insights into epigenetic regulation propose NSUN5/METTL1 axis therapeutic target for this malignancy.

Language: Английский

Persistent organic pollutant perfluorooctanoic acid induces alterations in epigenetic modifications of DNA and RNA DOI

Shu-Yi Gu,

Tian Feng,

Fang‐Yin Gang

et al.

Chinese Chemical Letters, Journal Year: 2025, Volume and Issue: unknown, P. 110957 - 110957

Published: Feb. 1, 2025

Language: Английский

Citations

4
Decoding N6-methyladenosine’s dynamic role in stem cell fate and early embryo development: insights into RNA–chromatin interactions DOI
Lei Yang,

Ming‐Li Ma,

Yawei Gao

et al.

Current Opinion in Genetics & Development, Journal Year: 2025, Volume and Issue: 91, P. 102311 - 102311

Published: Feb. 4, 2025

Language: Английский

Citations

0
A novel defined risk signature of ferroptosis-related lncRNAs for predicting prognosis, immune infiltration, and chemotherapy response in multiple myeloma DOI Creative Commons
Wei Yu,

Zizi Jing,

Jialin Tang

et al.

Discover Oncology, Journal Year: 2025, Volume and Issue: 16(1)

Published: Feb. 11, 2025

Language: Английский

Citations

0
Dynamic WNT signaling controls differentiation of hematopoietic progenitor cells from human pluripotent stem cells DOI
Mo Li, Keiichiro Suzuki, Mengge Wang

et al.

Science China Life Sciences, Journal Year: 2025, Volume and Issue: unknown

Published: March 11, 2025

Language: Английский

Citations

0
5-Methylcytosine methylation predicts cervical cancer prognosis, shaping immune cell infiltration DOI Creative Commons

Luyang Su,

Ren Xu,

Yanan Ren

et al.

Journal of International Medical Research, Journal Year: 2025, Volume and Issue: 53(4)

Published: April 1, 2025

BackgroundEpigenetics, encompassing DNA and RNA modifications, has emerged as a prominent area of research in the post-genomic era. Numerous studies have elucidated impact epigenetics on tumor regulation. However, methylation patterns 5-methylcytosine cervical cancer well its role microenvironment immunotherapy remain poorly understood.MethodsUtilizing comprehensive dataset samples from 306 patients with The Cancer Genome Atlas Gene Expression Omnibus repositories, we conducted an in-depth analysis to evaluate potential association between modification infiltration cells within microenvironment, taking into account 11 regulators modification. Subsequently, employed stepwise regression Least Absolute Shrinkage Selection Operator Cox quantify squamous cell carcinoma endocervical adenocarcinoma, yielding score. Our study explored link score clinical characteristics prognostic outcomes adenocarcinoma.ResultsA revealed two distinct patterns, henceforth labeled clusters A B. These exhibited immunological profiles biological attributes, cluster exhibiting higher degree immune infiltration. Utilizing univariate analysis, identified 367 genes regulated by that were significantly correlated patient prognosis. This further stratified three genomic subtypes. Survival analyses indicated belonging gene C more favorable survival than those Intriguingly, most regulatory factors had expression levels B A. set enrichment single sample elevated B, indicating stronger response this cluster. feature was utilized determine pattern cancer, revealing low scores better rates, whereas high increased mutation frequencies treatment responses.ConclusionsThis underscores key cancer. Analysis these will deepen our understanding paving way for development refined effective strategies.

Language: Английский

Citations

0
Targeting tRNA methyltransferases: from molecular mechanisms to drug discovery DOI
Yanrong Gao, Xin Yu Liu, Jiazhi Li

et al.

Science China Life Sciences, Journal Year: 2025, Volume and Issue: unknown

Published: May 7, 2025

Language: Английский

Citations

0
NOP2‐Mediated m5C Methylation Modification of LMNB2 mRNA Facilitates Colorectal Cancer Progression DOI Creative Commons

Jinling Bi,

Yong Huang, Wentao Hu

et al.

Cancer Medicine, Journal Year: 2025, Volume and Issue: 14(10)

Published: May 1, 2025

ABSTRACT Background Colorectal cancer (CRC) is a leading cause of cancer‐related mortality globally, yet current therapies exhibit suboptimal efficacy with limited prognostic improvement. RNA 5‐methylcytosine (m5C), posttranscriptional modification, has been implicated in tumorigenesis and progression across malignancies. In our previous study, the m5C methyltransferase NOP2 shown to promote proliferation, migration, invasion CRC cells, however, underlying mechanism still elusive. Methods An integrated multi‐omics strategy was employed, combining transcriptomic sequencing, immunoprecipitation sequencing (RIP‐seq), methylated (MeRIP‐seq) explore NOP2‐regulated downstream genes mediating via methylation. Functional validation included vitro vivo assays assess tumor growth metastasis. Rescue experiments were performed by overexpressing LMNB2 NOP2‐silenced cells. Results NOP2‐dependent modification mRNA enhanced its stability, elevated protein levels. This drove metastasis both vivo. Overexpression effectively rescued suppressed malignant phenotypes induced knockdown, confirming as critical effector. Conclusion catalyzes facilitate which contributes level progression, suggesting potential therapeutic target development novel treatment.

Language: Английский

Citations

0
Bisulfite-free whole-genome mapping of 5-methylcytosine at single-base resolution by NTD-seq DOI
Min Wang,

Neng‐Bin Xie,

Fang‐Yin Gang

et al.

Science China Life Sciences, Journal Year: 2025, Volume and Issue: unknown

Published: May 22, 2025

Language: Английский

Citations

0
Identification of key regulatory factors for m6A in myasthenia gravis and characteristics of the immune characteristics DOI Creative Commons

Yaoqi Wu,

Xiaoqing Cai,

Yingying Jiao

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 20, 2024

Abstract Myasthenia gravis (MG), a rare autoimmune disorder, presents complex pathogenesis involving various immune molecules. The modification of N6-methyladenosine (m6A) regulates diverse metabolic and immunopathological processes; however, its role in MG remains unclear. We downloaded dataset GSE85452 from the GEO database to identify differentially expressed genes regulated by m6A. Random Forest (RF) method was utilized pivotal regulatory associated with m6A modification. Subsequently, prognostic model crafted confirmed using this gene set. Patients were stratified according expression levels these key genes. Additionally, MG-specific signatures delineated examining cell infiltration patterns their correlations. Further functional annotation, protein-protein interaction mapping, molecular docking analyses performed on biomarkers, leading discovery three that exhibited significant differential within dataset: RBM15, CBLL1, YTHDF1.The random forest algorithm as MG, validated constructing clinical prediction model. Based expression, we divided patients into two groups, revealing distinct varying abundances. also discovered 61 phenotype conducted an in-depth exploration biological roles. YTHDF1 found positively correlated CD56dim natural killer cells, T type 1 helper cells. These stable diagnostic m6A-related markers both validation cohorts. Our findings suggest for MG. analysis may elucidate roles microenvironment

Language: Английский

Citations

0
RNA Methyltransferase NSUN5 Promotes Esophageal Cancer via 5‐Methylcytosine Modification of METTL1 DOI Open Access

YuanBo Cui,

Zhaoyang Hu, Chunyan Zhang

et al.

Molecular Carcinogenesis, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 27, 2024

Aberrant RNA modifications can drive carcinogenic transformation and tumor progression, with 5-methylcytosine (m5C) emerging as one of the predominant in eukaryotic cells. However, function molecular mechanisms m5C esophageal cancer (ESCA) remain insufficiently defined. Here we report that methyltransferase NOP2/Sun domain family member 5 (NSUN5) is significantly upregulated ESCA tumors shows promising diagnostic potential. Functionally, knockdown NSUN5 impairs proliferation capacity cells arrests cell cycle at G0/G1 phase, while enforced expression accelerates progression. In vivo, deficiency reduces growth a cell-based xenograft mouse model. Mechanistically, correlates oncogenic like 1 (METTL1), positively regulating its expression; binds directly to METTL1 transcript, facilitating modification Additionally, overexpression effectively counteracts tumor-suppressive effects resulting from ablation both vitro vivo settings. A comprehensive pan-cancer analysis further underscores NSUN5's essential role digestive system tumors, downregulation notably inhibiting gastric colon growth. These findings provide new insights into epigenetic regulation propose NSUN5/METTL1 axis therapeutic target for this malignancy.

Language: Английский

Citations

0