Medicinal Plant Biology, Journal Year: 2020, Volume and Issue: 0(0), P. 1 - 16
Published: Jan. 1, 2020
Language: Английский
Plant Methods, Journal Year: 2025, Volume and Issue: 21(1)
Published: March 14, 2025
The root apical meristem (RAM) and the shoot (SAM), which contain stem cells their lineages, are centers for underground aboveground organ formation in vascular plants. Accurately tracing cell lineage is important to understand how RAM SAM formed they give rise new organs. Here, we introduce all-in-one CRE/LOX system, assembled a single plasmid can be used efficiently trace during de novo of meristems differentiation within Arabidopsis thaliana. Using CRE/LOX-GUS system driven by WUSCHEL-RELATED HOMEOBOX5 (WOX5) promoter (i.e., WOX5pro-CRE/LOX-GUS), show that division quiescent center (QC) niche might replenish initial/stem plants grown on mannitol-containing medium or soil. results obtained using WOX5pro-CRE/LOX-GUS also group progenitor acts together initiate regeneration from callus tissue culture. We demonstrate use CRE/LOX-RUBY real-time vivo lineages live Overall, this paper not only introduces tool, but reveals development regeneration.
Language: Английский
Citations
2
Science China Life Sciences, Journal Year: 2025, Volume and Issue: unknown
Published: Jan. 8, 2025
Abstract Epigenetic mechanisms are integral to plant growth, development, and adaptation environmental stimuli. Over the past two decades, our comprehension of these complex regulatory processes has expanded remarkably, producing a substantial body knowledge on both locus-specific genome-wide patterns. Studies initially grounded in model Arabidopsis have been broadened encompass diverse array crop species, revealing multifaceted roles epigenetics physiological agronomic traits. With recent technological advancements, epigenetic regulations at single-cell level large-scale population emerging as new focuses. This review offers an in-depth synthesis regulations, detailing catalytic machinery functions. It delves into intricate interplay among various elements their collective influence modulation Furthermore, it examines breakthroughs technologies for modifications integration strategies improvement. The underscores transformative potential bolstering performance, advocating development efficient tools fully exploit agricultural benefits insights.
Language: Английский
Citations
1
Nature Plants, Journal Year: 2025, Volume and Issue: unknown
Published: Jan. 31, 2025
Language: Английский
Citations
1
Advanced Science, Journal Year: 2025, Volume and Issue: unknown
Published: Jan. 23, 2025
Abstract Plants exhibit remarkable regenerative abilities under stress conditions like injury, herbivory, and damage from harsh weather, particularly through adventitious root formation. They have sophisticated molecular mechanisms to recognize respond wounding. Jasmonic acid (JA), a wound hormone, triggers auxin synthesis stimulate regeneration. Melatonin (MT), structurally similar auxin, also significantly influences induction, but its specific mechanism is unclear. Phytomelatonin's signal transduction discovered in wound‐induced formation, identifying SlPMTR1/2 as phytomelatonin receptors, transmitting signals SHOOT BORNE ROOTLESS 1 (SlSBRL1), key regulator of regeneration, via the G protein α subunit (SlGPA1). Additionally, activated by JA, targeted SlMYC2. Overall, on regeneration uncovered revealed crosstalk between offering new insights into plant repair mechanisms.
Language: Английский
Citations
0
PLANT PHYSIOLOGY, Journal Year: 2025, Volume and Issue: 197(2)
Published: Jan. 31, 2025
A strong association between somatic embryogenesis competence and lipid accumulation in the embryogenic callus of citrus may facilitate plant regeneration germplasm utilization.
Language: Английский
Citations
0
Plant Methods, Journal Year: 2025, Volume and Issue: 21(1)
Published: March 13, 2025
Abstract Background Camelina is an oilseed crop with particularly useful fatty acid and amino profiles of its seeds, high resilience to abiotic biotic stresses, a short life cycle. Previous genetic engineering approaches in camelina have largely relied on the floral dip method which is, however, associated genotype-dependent efficiency incompatibility methods direct biomolecule delivery. Results Here, we established novel transgenesis for camelina, taking advantage regenerative capacity immature embryos. Various culture conditions treatments were experimentally validated, included duration explant pre-cultivation, wounding explants time application, Agrobacterium strain density inoculum, acetosyringone concentration, explant- co-cultivation, as well application concentration selective agent hygromycin. We provide convergent evidence stable transgenicity transgene inheritance by (1) selection resistance hygromycin, (2) PCR, (3) detection product GFP, (4) DNA gel blot analysis involving primary transgenic plants segregating progeny. Primary transgenics examined detail featured one three T-DNA integration loci, seven copies being integrated total per plant. The proved efficient across all tested accessions including two current cultivars, whereby transformation efficiencies, determined PCR-positive related agro-inoculated explants, between 13 17% obtained. Conclusion With this method, viable platform functional validation genes-of-interest biotechnological improvements plant performance quality features camelina.
Language: Английский
Citations
0
Plant Cell & Environment, Journal Year: 2025, Volume and Issue: unknown
Published: March 28, 2025
ABSTRACT Plant stem cells, residing in the shoot and root apical meristems, are fundamental for continuous growth organ formation throughout plant life cycle. Their regulation is driven by convergence of endogenous developmental cues exogenous environmental signals, making them pivotal to overall development. Auxin, a key phytohormone, serves as major internal signal, orchestrating cell initiation, maintenance, differentiation, adaptation through intricate biosynthesis, transport, signaling networks. This review summarizes recent progress understanding cellular molecular mechanisms which auxin guides functions both meristems. Through these insights, we explore how plants utilize auxin‐driven pathways optimize ever‐changing environments.
Language: Английский
Citations
0
Journal of Integrative Plant Biology, Journal Year: 2025, Volume and Issue: unknown
Published: April 11, 2025
ABSTRACT Plant regeneration is the process during which differentiated tissues or cells can reverse alter their developmental trajectory to repair damaged form new organs. In plant process, cell wall not only functions as a foundational barrier and scaffold supporting but also influences fates identities. Cell remodeling involves selective degradation of certain components integration components. Recently, accumulating evidence has underscored importance in regeneration. Wounding signals, transmitted by transcription factors, trigger expressions genes responsible for loosening, essential tissue repair. de novo organ somatic embryogenesis, phytohormones orchestrate transcriptional regulatory network induce remodeling, promotes fate reprogramming formation. This review summarizes effects on various regenerative processes provides novel insights into future research uncharacterized roles
Language: Английский
Citations
0
New Phytologist, Journal Year: 2025, Volume and Issue: unknown
Published: April 27, 2025
Summary In both plants and animals, tissue or organ regeneration typically follows wounding, which also activates defense responses against pathogenic microbes herbivores. Both intrinsic environmental cues guide the molecular decisions between defense. animal studies, extensive research has highlighted role of various – including pathogenic, commensal, beneficial species in influencing signaling interplay immunity regeneration. Conversely, most plant studies are conducted under sterile conditions, leaves a gap our understanding how innate influences pathways. Recent findings have begun to elucidate roles key pathways modulating crosstalk these two processes. These explore might influence choice plants. This review examines mechanisms governing balance immunity, with focus on emerging aging microbial interactions shaping
Language: Английский
Citations
0
Current Opinion in Plant Biology, Journal Year: 2025, Volume and Issue: 85, P. 102733 - 102733
Published: May 3, 2025
Language: Английский
Citations
0