Role of Chinese Medicine Monomers in Dry Eye Disease: Breaking the Vicious Cycle of Inflammation

Pharmacology Research & Perspectives, Journal Year: 2025, Volume and Issue: 13(2)

Published: Feb. 20, 2025

Dry eye disease (DED) is a chronically inflammatory ocular surface disorder of unknown pathogenesis. Anti-inflammatory medications, artificial tears, autologous serum, and LipiFlow have been shown to be highly beneficial in alleviating symptoms. Nevertheless, these interventions often provide only short-term results do not address the underlying problems disease. There growing evidence that risk DED associated with vicious cycle inflammation. This inflammation produced by interaction several factors, including tear film hyperosmolarity, instability, inflammation, apoptosis. Chinese medicine monomers, distinguished their multicomponent multitarget advantages, help treat modulating status, inhibiting responses, apoptosis, providing new way thinking management medicine.

Language: Английский

---

Biased agonism of G protein-coupled receptors as a novel strategy for osteoarthritis therapy

Bone Research, Journal Year: 2025, Volume and Issue: 13(1)

Published: May 12, 2025

Abstract Osteoarthritis (OA) is a prevalent degenerative joint disorder marked by chronic pain, inflammation, and cartilage loss, with current treatments limited to symptom relief. G protein-coupled receptors (GPCRs) play pivotal role in OA progression regulating chondrocyte survival, matrix homeostasis. However, their multifaceted signaling, via proteins or β-arrestins, poses challenges for precise therapeutic targeting. Biased agonism, where ligands selectively activate specific GPCR pathways, emerges as promising approach optimize efficacy reduce side effects. This review examines biased signaling OA-associated GPCRs, including cannabinoid (CB 1 , CB 2 ), chemokine (CCR2, CXCR4), protease-activated (PAR-2), adenosine (A R, A 2A 2B 3 R), melanocortin (MC MC bradykinin (B prostaglandin E (EP-2, EP-4), calcium-sensing (CaSR). We analyze clinical trials explore natural products from Traditional Chinese Medicine potential agonists. These compounds, diverse structures bioactivities, offer novel avenues. By harnessing this underscores the developing targeted, safer therapies that address its complex pathology, bridging molecular insights translation.

Language: Английский

---

Biological Evaluation of Lysionotin: a Novel Inhibitor of 5-Lipoxygenase for Anti-glioma

Chinese Journal of Integrative Medicine, Journal Year: 2024, Volume and Issue: 30(9), P. 826 - 834

Published: July 11, 2024

Language: Английский

---

Identification of stable reference genes for quantitative real-time PCR in human fibroblasts from lymph nodes and synovium

Published: July 12, 2024

Abstract Real-time quantitative PCR (RT–qPCR) has emerged as an accurate and widely used technique for measuring gene expression levels. However, its reliability depends on the selection of appropriate reference genes to normalize sample input. Accordingly, identification characterized by stable in cells conditions interest is essential ensuring values. To date, no study specifically identified suitable primary human cultured fibroblast-like synoviocytes (FLS) lymph node stromal (LNSCs) within context rheumatoid arthritis (RA). These play a critical role pathogenesis disease. In this study, we evaluated suitability 15 candidate normalizing transcript FLS LNSCs subjected various vitro stimuli. We included traditional often normalization fibroblasts well via GeneVestigator analysis publicly available transcriptomic data. RefFinder algorithms were identify most across cell types different experimental conditions. determined that optimal number every condition tested was two; RPLP0 POLR2G exhibited greatest stability LNSCs. FLS, observed greater variability Although TBP under unstimulated conditions, our findings indicated require distinct depending specific Validation selected levels metabolic emphasized importance prior evaluation potential genes, arbitrary could lead data misinterpretation. This constitutes first systematic selecting fibroblasts. Our emphasize proper each separately when applying standard technology assessing

Language: Английский

---

Advances in understanding the role of interleukins in pulmonary fibrosis (Review)

Experimental and Therapeutic Medicine, Journal Year: 2024, Volume and Issue: 29(2)

Published: Nov. 28, 2024

Pulmonary fibrosis (PF) is a progressive, irreversible disease characterized by heterogeneous interstitial lung tissue damage. It originates from persistent or repeated epithelial injury and leads to the activation differentiation of fibroblasts into myofibroblasts. Interleukins (ILs) are group lymphokines crucial for immunomodulation that implicated in pathogenesis PF. However, different types ILs exert disparate effects on In present review, based effect PF, classified three categories: i) Promotors PF; ii) inhibitors iii) those dual Several can promote PF provoking inflammation, initiating proliferation transdifferentiation cells, exacerbating injury, while other inhibit through suppressing expression inflammatory factors, modulating Th1/Th2 balance autophagy. The review summarizes association focusing roles mechanisms underlying

Language: Английский

---