Terroir-driven flavor, microbiome, and metabolome signatures of Jiaoke: A multi-omics exploration of traditional fermented dairy from Inner Mongolia

Food Bioscience, Journal Year: 2025, Volume and Issue: unknown, P. 105922 - 105922

Published: Jan. 1, 2025

Language: Английский

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Genomic profiling of pediococcus acidilactici BCB1H and identification of its key features for Biotechnological innovation, food technology and medicine

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: Feb. 19, 2025

Lactic acid bacteria has been extensively used in food industry because of widespread properties and Pediococcus is among one them. This study aims to conduct a comprehensive genomic analysis acidilactici strain BCB1H elucidate its genetic composition, functional elements, potential biotechnological applications. The objectives include identifying key features such as coding sequences, tRNA rRNA genes, antibiotic resistance secondary metabolite biosynthetic gene clusters, which will highlight the adaptability P. for use variety industrial therapeutic was analyzed using whole-genome sequencing, advanced sequencing technologies obtain data. Key features, were identified through bioinformatics analyses. revealed genome size approximately 1.92 million base pairs with GC content 42.4%. annotation 1,895 genes across 192 subsystems, highlighting metabolic pathways categories. Notably, specialty associated carbohydrate metabolism, stress response, pathogenicity, amino synthesis identified, underscoring versatility applications technology medicine. These findings shed light on makeup BCB1H, flexibility importance. traits discovered suggest prospective probiotics, preservation, advancements.

Language: Английский

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Integrative Interpretation of Linoleic Acid Biotransformation by Probiotic Pediococcus acidilactici BCB1H via Metabolite Profiling and Enzyme Interaction Analysis

LWT, Journal Year: 2025, Volume and Issue: unknown, P. 117664 - 117664

Published: March 1, 2025

Language: Английский

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Pediococcus acidilactici BCB1H exopolysaccharide enhances growth, antioxidant activity, and gut health in juvenile turbot

Journal of Applied Animal Research, Journal Year: 2025, Volume and Issue: 53(1)

Published: March 20, 2025

This study investigated the potential benefits for juvenile turbot of Pediococcus acidilactici (BCB1H) exopolysaccharide (EPS). Juvenile turbots were randomly divided into three groups (100 per group), and different concentrations BCB1H-EPS added to their feed. After 7 weeks intervention, number status fish was recorded, intestine isolated. The antioxidant activities, gut enzymatic immune capacity, growth performance, intestinal flora measured. Compared control group, supplemented with significantly increased. Moreover, immunological activities in considerably enhanced EPS-fed groups. also increased secretion cytokines, improved presence probiotics, reduced abundance pathogenic bacteria gut, system, predicted attenuate some disease-related pathways. These findings indicate that adding diet can stimulate development increase while actively managing microbiota. Overall, results this investigation show promise use P. EPS as an effective stimulant aquatic feed improve performance enhance immunity.

Language: Английский

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Revolutionizing the probiotic functionality, biochemical activity, antibiotic resistance and specialty genes of Pediococcus acidilactici BCB1H via in-vitro and in-silico approaches

Zeitschrift für Naturforschung C, Journal Year: 2024, Volume and Issue: 80(3-4), P. 103 - 118

Published: July 19, 2024

This study presents a comprehensive genomic exploration, biochemical characterization, and the identification of antibiotic resistance specialty genes Pediococcus acidilactici BCB1H strain. The functional genetic makeup, biological activities, other considerable parameters have been investigated in this with prime focus on gene profiles. results revealed unique susceptibility patterns for genes. had good vitro probiotic properties, which survived well simulated artificial gastrointestinal fluid, exhibited acid bile salt resistance. didn't produce hemolysis certain sensitivity, making it relatively safe LAB Simultaneously, self-coagulation characteristics antioxidant activity. EPS produced by also activity hypoglycemic function. Moreover, genome 42.4 % GC content size roughly 1.92 million base pairs was analyzed investigations. annotation identified 192 subsystems 1,895 genes, offering light metabolic pathways categories found BCB1H. linked to metabolism carbohydrates, stress response, pathogenicity, amino acids highlighted strain's versatility possible uses. establishes groundwork future investigations highlighting significance using multiple strains investigate diversity experimental validation predicted provide roadmap utilizing P. BCB1H's traits industrial medical applications, opening door real-world uses industries including food technology medicine.

Language: Английский

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Comparative Study on Growth and Metabolomic Profiles of Six Lactobacilli Strains by Sodium Selenite

Microorganisms, Journal Year: 2024, Volume and Issue: 12(10), P. 1937 - 1937

Published: Sept. 24, 2024

Selenium (Se) has garnered increasing attention in the field of nutrition, as it is essential for both humans and animals. Certain microorganisms can enrich inorganic selenium convert into organic selenium. The growth metabolomic profiles six lactobacilli strains exposed to 50 μg/mL sodium selenite were performed using gas chromatography tandem time-off light mass spectrometry (GC-TOF-MS) analysis. addition significantly increased population weight Lacticaseibacillus rhamnosus PS5, Lbs. RT-B, Limosilactobacillus reuteri 3630, Lmb. 1663 strains, while those other two decreased. A total 271 metabolites determined, with their concentrations ranked from highest lowest follows: acids derivatives, oxygen compounds, lipids lipid-like molecules, benzenoids. In certain groups, serine, aspartic acid, trehalose, palmitic methylthreonine, melibiose significantly, whereas glucuronic ribose, ornithine, methionine downregulated. metabolic pathways associated ABC transporters, glycine, threonine metabolism, aminobenzoate degradation pathways. Based on these findings, we concluded that transport, absorption, assimilation, stress response by changed. Furthermore, alterations among different types varied primarily due distinct properties.

Language: Английский

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New insight into protective effect against oxidative stress and biosynthesis of exopolysaccharides produced by Lacticaseibacillus paracasei NC4 from fermented eggplant

Current Genetics, Journal Year: 2024, Volume and Issue: 70(1)

Published: May 14, 2024

Language: Английский

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Exopolysaccharides from Weissella cibaria isolated from oats: isolation, characterisation and its application in improving the texture of set‐type yogurt

International Journal of Food Science & Technology, Journal Year: 2024, Volume and Issue: 59(12), P. 9532 - 9546

Published: Sept. 9, 2024

Summary In this study, a lactic acid bacteria strain isolated from oats was used as producer to obtain exopolysaccharide (EPS) using MRS agar plates. The findings indicated that the bacterial identified Weissella cibaria (accession number: PP515290 ) shown have good probiotic activity, adapt better alkaline than acidic conditions, and efficiently produce EPS at concentrations of up 7.93 g L −1 . SEM images showed smooth surface compact particle whereas FTIR confirmed basic functional groups polysaccharides in sample. Meanwhile, XRD analysis demonstrated non‐crystalline amorphous structure thermal two‐phase decomposition with maximum rate 210 °C. Subsequently, W. applied stabiliser set‐type yogurt ( ex situ comparison mixed culture traditional starter (control). results addition 2% significantly improved syneresis (14.31%) compared co‐culture (27.50%) control (29.80%) well increased hardness during 2‐week refrigeration.

Language: Английский

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