Toxicity Assay and Pathogenic Process Analysis of Clonostachys rogersoniana Infecting Cephalcia chuxiongica DOI Creative Commons
Junjia Lu, Jian Liu, Huali Li

et al.

Microorganisms, Journal Year: 2025, Volume and Issue: 13(4), P. 709 - 709

Published: March 21, 2025

Cephalcia chuxiongica has caused significant damage to pine forests, becoming a major biological disaster that hinders the sustainable development of forestry in China. To investigate efficacy control measures, entomopathogenic fungi were isolated and purified from larvae Ce. had succumbed diseases. The pathogenic capacity strains was assessed using bioassay methods, their infection process observed scanning electron microscopy. ITS, LSU, TEF analysis disclosed Clonostachys rogersoniana as highly virulent strain responsible for death chuxiongica. optimal medium its mycelial growth sporulation found be PPDA. In addition, revealed median lethal time (LT50) 24.34 h concentration (LC50) 2.35 × 105 conidia/mL, indicating C. possesses potent virulence demonstrates rapid pathogenicity. Furthermore, microscopy demonstrated initially entered body through spiracle progressively made way into wall, resulting insect’s death. mode is exceedingly rare. As consequence, results this study can serve reference management chewing insects, such

Language: Английский

Toxicity Assay and Pathogenic Process Analysis of Clonostachys rogersoniana Infecting Cephalcia chuxiongica DOI Creative Commons
Junjia Lu, Jian Liu, Huali Li

et al.

Microorganisms, Journal Year: 2025, Volume and Issue: 13(4), P. 709 - 709

Published: March 21, 2025

Cephalcia chuxiongica has caused significant damage to pine forests, becoming a major biological disaster that hinders the sustainable development of forestry in China. To investigate efficacy control measures, entomopathogenic fungi were isolated and purified from larvae Ce. had succumbed diseases. The pathogenic capacity strains was assessed using bioassay methods, their infection process observed scanning electron microscopy. ITS, LSU, TEF analysis disclosed Clonostachys rogersoniana as highly virulent strain responsible for death chuxiongica. optimal medium its mycelial growth sporulation found be PPDA. In addition, revealed median lethal time (LT50) 24.34 h concentration (LC50) 2.35 × 105 conidia/mL, indicating C. possesses potent virulence demonstrates rapid pathogenicity. Furthermore, microscopy demonstrated initially entered body through spiracle progressively made way into wall, resulting insect’s death. mode is exceedingly rare. As consequence, results this study can serve reference management chewing insects, such

Language: Английский

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