Standardization of bone morphometry and mineral density assessments in zebrafish and other small laboratory fishes using x-ray radiography and micro–computed tomography DOI Creative Commons
Érika Kague, Ronald Y. Kwon, Björn Busse

et al.

Journal of Bone and Mineral Research, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 30, 2024

Abstract Zebrafish and other small laboratory fishes are emerging as important animal models for investigating human skeletal development diseases. In recent years, there has been a notable increase in research publications employing X-ray radiography micro-computed tomography to analyze the structures of these animals. However, evaluating bone morphology mineral density fish poses unique challenges compared well-established rodent models. The varied approaches image acquisition, analysis, reporting across studies have led substantial obstacles interpreting comparing findings. This article addresses urgent need standardized parameters methodologies related acquisition well adoption harmonized nomenclature. Furthermore, it offers guidance on anatomical terminology, units measurement, establishment minimal reporting, along with comprehensive documentation methods algorithms used analysis. We anticipate that adherence guidelines will enhance consistency, reproducibility, interpretability reported measurements morphometry These advancements vital accurately phenotypes gene functions, particularly context multi-center studies.

Language: Английский

Lipid peroxidation inhibition by icaritin and its glycosides as a strategy to combat iron overload-induced osteoporosis in zebrafish DOI
Chang‐Yu Yan,

Xiao‐yuan Gu,

Si Tan

et al.

Food Research International, Journal Year: 2025, Volume and Issue: 203, P. 115900 - 115900

Published: Feb. 1, 2025

Language: Английский

Citations

0
Standardization of bone morphometry and mineral density assessments in zebrafish and other small laboratory fishes using x-ray radiography and micro–computed tomography DOI Creative Commons
Érika Kague, Ronald Y. Kwon, Björn Busse

et al.

Journal of Bone and Mineral Research, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 30, 2024

Abstract Zebrafish and other small laboratory fishes are emerging as important animal models for investigating human skeletal development diseases. In recent years, there has been a notable increase in research publications employing X-ray radiography micro-computed tomography to analyze the structures of these animals. However, evaluating bone morphology mineral density fish poses unique challenges compared well-established rodent models. The varied approaches image acquisition, analysis, reporting across studies have led substantial obstacles interpreting comparing findings. This article addresses urgent need standardized parameters methodologies related acquisition well adoption harmonized nomenclature. Furthermore, it offers guidance on anatomical terminology, units measurement, establishment minimal reporting, along with comprehensive documentation methods algorithms used analysis. We anticipate that adherence guidelines will enhance consistency, reproducibility, interpretability reported measurements morphometry These advancements vital accurately phenotypes gene functions, particularly context multi-center studies.

Language: Английский

Citations

0