Regenerative Outcomes of Combining siCOL1A2 Hydrogel with Acupuncture in a Rat Model of Chronic Intervertebral Disc Degeneration DOI Creative Commons

Qianfu Zhang,

Zhixuan Li,

Sihan Zhou

et al.

Bioengineering, Journal Year: 2024, Volume and Issue: 11(11), P. 1066 - 1066

Published: Oct. 25, 2024

Intervertebral disc degeneration (IVDD) is a significant cause of chronic pain and disability, necessitating innovative therapeutic strategies. This study investigates the combined effect novel siCOL1A2-encapsulated hydrogel acupuncture on IVDD in rat model. We developed system, G5-PBA (siCOL1A2@G5-PBA@Gel), designed for sustained siRNA delivery to degenerated discs assessed its efficacy alongside treatment. Key inflammatory genes were identified through RNA-seq analysis, with COL1A2 highlighted as crucial regulator responses IVDD. Our vivo experiments involved treating rats alone, combining both. The treatments evaluated behavioral assessments, imaging techniques (X-ray MRI), histological analyses. Results indicated that combination therapy significantly alleviated pain, reduced inflammation, promoted regeneration more effectively than individual treatments. proved biocompatible facilitated targeted gene silencing, while enhanced outcomes by improving local blood circulation modulating responses. These findings suggest integrating siCOL1A2 offers promising approach

Language: Английский

Regenerative Outcomes of Combining siCOL1A2 Hydrogel with Acupuncture in a Rat Model of Chronic Intervertebral Disc Degeneration DOI Creative Commons

Qianfu Zhang,

Zhixuan Li,

Sihan Zhou

et al.

Bioengineering, Journal Year: 2024, Volume and Issue: 11(11), P. 1066 - 1066

Published: Oct. 25, 2024

Intervertebral disc degeneration (IVDD) is a significant cause of chronic pain and disability, necessitating innovative therapeutic strategies. This study investigates the combined effect novel siCOL1A2-encapsulated hydrogel acupuncture on IVDD in rat model. We developed system, G5-PBA (siCOL1A2@G5-PBA@Gel), designed for sustained siRNA delivery to degenerated discs assessed its efficacy alongside treatment. Key inflammatory genes were identified through RNA-seq analysis, with COL1A2 highlighted as crucial regulator responses IVDD. Our vivo experiments involved treating rats alone, combining both. The treatments evaluated behavioral assessments, imaging techniques (X-ray MRI), histological analyses. Results indicated that combination therapy significantly alleviated pain, reduced inflammation, promoted regeneration more effectively than individual treatments. proved biocompatible facilitated targeted gene silencing, while enhanced outcomes by improving local blood circulation modulating responses. These findings suggest integrating siCOL1A2 offers promising approach

Language: Английский

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