Supercritical carbon-dioxide (SC-CO2) extraction of lipids and carotenoids from Rhodotorula toruloides CBS 14 in comparison with conventional extraction methods DOI Creative Commons
Yashaswini Nagavara Nagaraj,

Johanna Blomqvist,

Sabine Sampels

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 16, 2024

Abstract Background We conducted extractions using supercritical carbon dioxide (SC-CO2) and conventional solvent methods to extract lipids carotenoids from R. toruloides CBS 14 cells grown on wheat straw hydrolysate. The lipid extracts were analyzed gas chromatography (GC), the identified quantified ultra-high performance liquid (UHPLC). Results Four main in both extraction including β-carotene, γ-carotene, torularhodin, torulene. Interestingly, torularhodin was major carotenoid extracted SC-CO2 extraction, followed by This different acetone method, where β-carotene carotenoid. After torulene underwent degradation due saponification step, which necessary remove before UHPLC analysis. total concentration obtained 332.09 ± 27.32 μg/g dry weight compared 19.9 2.74 extraction. A small amount of observed be lost into extract, but this loss not as substantial that seen with Additionally, content samples significantly lower than Folch method. GC analysis revealed oleic acid fatty extracts, palmitic linoleic acid. Notably, proportion unsaturated acids higher method Conclusion These findings indicate outperformed preserving integrity retaining an abundance carotenoids, resulting high-quality extracts.

Language: Английский

Green extraction of carotenoids and oil produced by Rhodosporidium paludigenum using supercritical CO2 extraction: Evaluation of cell disruption methods and extraction kinetics DOI Creative Commons

Fani Sereti,

Maria Alexandri, Harris Papapostolou

et al.

Food Chemistry, Journal Year: 2025, Volume and Issue: unknown, P. 144261 - 144261

Published: April 1, 2025

The constantly expanding functional food market has steered scientific research towards alternative sources of bioactive compounds. Red yeasts are valuable producers active ingredients such as carotenoids and microbial oil. Efficient sustainable recovery methods required when applications targeted. In this study, intracellular oil synthesized by Rhodosporidium paludigenum in batch bioreactor cultures were recovered using supercritical CO2 (SFE-CO2) a green to conventional organic solvents. Yeast biomass was subjected six different cell disruption prior SFE-CO2. Homogenization emerged the optimal pre-treatment method, resulting an 80 % yield total 83 use ethanol co-solvent imperative for efficient both products. β-Carotene main carotenoid, while obtained rich oleic acid. These results pave way integrating these compounds into innovative

Language: Английский

Citations

0
Supercritical carbon dioxide extraction of lipids and carotenoids from Rhodotorula toruloides CBS 14 in comparison with conventional extraction methods DOI Creative Commons
Yashaswini Nagavara Nagaraj,

Johanna Blomqvist,

Sabine Sampels

et al.

Biotechnology for Biofuels and Bioproducts, Journal Year: 2025, Volume and Issue: 18(1)

Published: March 21, 2025

Abstract Background Oil from oleaginous yeasts has the potential to replace non-sustainable vegetable oil as raw material produce food, feed, biofuels, or biochemicals. Co-produced compounds like carotenoids may be helpful obtain economically viable bioprocesses. Identifying appropriate extraction methods is a bottleneck both for establishing cell factories and production analysis of intracellular lipids carotenoids. We conducted extractions using supercritical carbon dioxide (SC-CO 2 ) conventional solvent extract analyze R. toruloides CBS 14 cells grown on wheat straw hydrolysate. The lipid extracts were analyzed gas chromatography (GC), identified quantified ultra-high-performance liquid (UHPLC). Results Four main in including β-carotene, γ-carotene, torularhodin, torulene. Interestingly, torularhodin was major carotenoid extracted SC-CO extraction, followed by This different acetone method, where β-carotene carotenoid. After torulene underwent degradation due saponification step, which necessary remove before UHPLC analysis. total concentration obtained 332.09 ± 27.32 μg/g dry weight compared 19.9 2.74 extraction. A small amount observed lost into extract, but this loss not substantial that seen with Additionally, content samples significantly lower than Folch method. GC revealed oleic acid fatty extracts, palmitic linoleic acid. Notably, proportion unsaturated acids higher method Conclusion These findings indicate outperformed preserving integrity retaining an abundance carotenoids, resulting high-quality extracts.

Language: Английский

Citations

0
Microbial production of torulene and its potential applications: a review DOI
Jiahui Jin, Jun Li, Yanming Qiao

et al.

Food Science and Biotechnology, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 9, 2024

Language: Английский

Citations

1
Supercritical carbon-dioxide (SC-CO2) extraction of lipids and carotenoids from Rhodotorula toruloides CBS 14 in comparison with conventional extraction methods DOI Creative Commons
Yashaswini Nagavara Nagaraj,

Johanna Blomqvist,

Sabine Sampels

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 16, 2024

Abstract Background We conducted extractions using supercritical carbon dioxide (SC-CO2) and conventional solvent methods to extract lipids carotenoids from R. toruloides CBS 14 cells grown on wheat straw hydrolysate. The lipid extracts were analyzed gas chromatography (GC), the identified quantified ultra-high performance liquid (UHPLC). Results Four main in both extraction including β-carotene, γ-carotene, torularhodin, torulene. Interestingly, torularhodin was major carotenoid extracted SC-CO2 extraction, followed by This different acetone method, where β-carotene carotenoid. After torulene underwent degradation due saponification step, which necessary remove before UHPLC analysis. total concentration obtained 332.09 ± 27.32 μg/g dry weight compared 19.9 2.74 extraction. A small amount of observed be lost into extract, but this loss not as substantial that seen with Additionally, content samples significantly lower than Folch method. GC analysis revealed oleic acid fatty extracts, palmitic linoleic acid. Notably, proportion unsaturated acids higher method Conclusion These findings indicate outperformed preserving integrity retaining an abundance carotenoids, resulting high-quality extracts.

Language: Английский

Citations

0