Risk Element Interaction with an Emerging Contaminant in Mammals: The Case of Arsenic and Carbamazepine Orally Taken up by Microtus arvalis L. as a Model Organism

Applied Sciences, Journal Year: 2025, Volume and Issue: 15(3), P. 1662 - 1662

Published: Feb. 6, 2025

Arsenic (As), a well-known ‘traditional’ environmental contaminant, and carbamazepine (CBZ), an emerging contaminant of pharmaceutical category, are both frequently detected in the environment have been shown to exhibit toxicity at exposure concentrations present environment. This study aimed assess single combined effects these pollutants on adult common vole (Microtus arvalis L.), model mammalian organism. assessed As CBZ accumulation, their biotransformation processes, antioxidant enzyme defence responses after sole exposure. The animals were fed diet containing either low (1.25 mg/kg) or high level (166 As. Moreover, half exposed via drinking water infused with 10 ng/L CBZ, second had use devoid CBZ. results showed enhanced total species contents organs + group compared group. High did not cause uptake liver There was potential accumulation groups (CBZ CBZ), raising concerns about toxic mammals from long-term Glutathione peroxidase (GPx) activity, reflecting against co-exposure two pollutants, that exhibited comparable activity control group, while down-regulation up-regulation. These findings suggest experienced minimal oxidative stress conditions, similar rapid adaptation response curtail offset tissue damage slow adaptation/response this experiment indicate possible interactions various could alter individual necessity investigating for better understanding risk real conditions.

Language: Английский

Chronic Exposure to Arsenic and Fluoride Starting at Gestation Alters Liver Mitochondrial Protein Expression and Induces Early Onset of Liver Fibrosis in Male Mouse Offspring

Biological Trace Element Research, Journal Year: 2024, Volume and Issue: unknown

Published: April 27, 2024

Abstract The presence of arsenic (As) and fluoride (F − ) in drinking water is concern due to the enormous number individuals exposed this condition worldwide. Studies cultured cells animal models have shown that As- or F-induced hepatotoxicity primarily associated with redox disturbance altered mitochondrial homeostasis. To explore hepatotoxic effects chronic combined exposure As F water, pregnant CD-1 mice were 2 mg/L (sodium arsenite) and/or 25 fluoride). male offspring continued treatment up 30 (P30) 90 (P90) postnatal days. GSH levels, cysteine synthesis enzyme activities, transporter levels investigated liver homogenates, as well expression biomarkers ferroptosis biogenesis-related proteins. Serum transaminase Hematoxylin–Eosin Masson trichrome-stained tissue slices examined. Combined at P30 significantly reduced transcription factor A (TFAM) while increasing lipid peroxidation, free Fe 2+ , p53 expression, serum ALT activity. At P90, upregulation uptake was a recovery levels. Nevertheless, downregulation TFAM now downstream inhibition MT-CO2 mtDNA fibrotic damage. Our experimental approach using human-relevant doses gives evidence increased risk for early damage elevated diet during intrauterine period.

Language: Английский