Cumin (Cuminum cyminum L.) seeds accelerates wound healing in rats: Possible molecular mechanisms

Skin Research and Technology, Journal Year: 2024, Volume and Issue: 30(5)

Published: May 1, 2024

Abstract Wound healing is a complex, intricate, and dynamic process that requires effective therapeutic management. The current study evaluates the wound potentials of methanolic extract Cuminum cyminum L. seeds (CCS) in rats. Sprague Dawley (24) rats were distributed into four cages, wounds produced on back neck, received two daily topical treatments for 14 days: A, normal saline; B, wounded treated with intrasite gel; C D, 0.2 mL 250 500 mg/kg CCS, respectively. After that, area closure percentage evaluated, tissues dissected histopathological, immunohistochemical, biochemical examinations. Acute toxicity trials CCS showed absence any physiological changes or mortality application caused significant reduction size statistically elevated contraction than those vehicle treatment up‐regulation collagen fiber, fibroblasts, fewer inflammatory cells (inflammation) granulation tissues. TGF‐β1 (angiogenetic factor) was significantly more expressed CCS‐treated comparison to saline‐treated rats; therefore, fibroblasts transformed myofibroblasts (angiogenesis). remarkable antioxidant (higher SOD CAT enzymes) decreased MDA (lipid peroxidation) levels their tissue homogenates. Hydroxyproline amino acid (collagen) up‐regulated by treatment, which commonly related faster area. outcomes suggest as viable new source pharmaceuticals treatment.

Language: Английский

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A triterpenoid (corosolic acid) ameliorated AOM-mediated aberrant crypt foci in rats: modulation of Bax/PCNA, antioxidant and inflammatory mechanisms

Journal of Molecular Histology, Journal Year: 2024, Volume and Issue: 55(5), P. 765 - 783

Published: Aug. 10, 2024

Language: Английский

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A bitter flavonoid gum from Dorema aucheri accelerate wound healing in rats: Involvement of Bax/HSP 70 and hydroxyprolin mechanisms

Skin Research and Technology, Journal Year: 2024, Volume and Issue: 30(8)

Published: Aug. 1, 2024

Abstract Background Dorema aucheri gum (DAG) is a bitter flavonoid widely used for numerous medicinal purposes including wound recovery. The present work investigates the acute toxicity and wound‐healing effects of DAG in excisional skin injury rats. Materials methods Sprague Dawley rats (24) were clustered into four groups, each rat had full‐thickness dorsal neck (2.00 cm) addressed with 0.2 mL following treatments 15 days: Group A (vehicle), normal saline; B, received intrasite gel; C D, 250 500 mg/kg DAG, respectively. Results results revealed absence any toxic signs who oral dosages 2 5 g/kg DAG. Wound healing was significantly accelerated indicated by smaller open areas higher contraction percentages compared to vehicle Histological evaluation fibroblast formation, collagen deposition, noticeably lower inflammatory cell infiltration granulated tissues DAG‐addressed treatment caused significant modulation immunohistochemical proteins (decreased Bax increased HSP 70) mediators (reduced TNF‐α, IL‐6, magnified IL‐10), which varied Moreover, topical led upregulation hydroxyproline (HDX) (collagen) antioxidant content. At same time, decreased lipid peroxidation (MDA) levels healed obtained from DAG‐treated Conclusion might be linked modulatory effect its phytochemicals (polysaccharides, flavonoids, phenolic) on cellular mechanisms, justify their folkloric use provokes further investigation as therapeutic drug additives contraction.

Language: Английский

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Exploring the Role of Boron-Containing Compounds in Biological Systems: Potential Applications and Key Challenges

Journal of Trace Elements in Medicine and Biology, Journal Year: 2025, Volume and Issue: 87, P. 127594 - 127594

Published: Jan. 10, 2025

Language: Английский

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Pinostrobin attenuates azoxymethane-induced colorectal cytotoxicity in rats through augmentation of apoptotic Bax/Bcl-2 proteins and antioxidants

SAGE Open Medicine, Journal Year: 2023, Volume and Issue: 11

Published: Jan. 1, 2023

Pinostrobin (5-hydroxy-7-methoxyflavanone; PN) is a natural active ingredient with numerous biological activities extensively utilized in tumour chemotherapy. The present study investigates the chemo-preventive potentials of PN on azoxymethane-mediated colonic aberrant crypt foci rats.Sprague Dawley rats clustered into five groups, normal control (A) and cancer controls were subcutaneously injected saline 15 mg/kg azoxymethane, respectively, nourished 10% tween 20 fed 20; reference (C), azoxymethane (intraperitoneal) 35 5-fluorouracil (5-FU); D E rat groups received subcutaneous injection 30 60 PN, respectively.The acute toxicity trial showed lack any abnormal signs or mortality ingested 250 500 PN. gross morphology colon tissues revealed significantly lower total incidence PN-treated than that controls. Histological examination increased availability bizarrely elongated nuclei, stratified cells higher depletion submucosal glands treatment caused positive modulation apoptotic (Bax Bcl-2) proteins inflammatory cytokines (TNF-α, IL-6 IL-10). Moreover, had antioxidants (superoxide dismutase) malondialdehyde concentrations their tissue homogenates.The chemoprotective efficiency against azoxymethane-induced shown by values inhibition percentage, possibly through augmentation genes responsible for cascade inflammations originating from oxidative stress insults.

Language: Английский

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Ephedra intermedia alleviates ethanol-mediated gastric ulcer in rats by anti-inflammatory and antioxidant mechanisms

Baghdad Science Journal, Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 19, 2024

تم استخدام Ephedra intermedia كعلاج تقليدي لأغراض صحية مختلفة بما في ذلك اضطرابات المعدة. للتحقق من صحة استخدامه التقليدي، قمنا بدراسة السمية الحادة والتأثيرات الوقائية المعدية لمستخلص أوراق (EILE) إصابة المعدة الناجمة عن الإيثانول الفئران. فصل الفئران سبراغ داولي بشكل عشوائي إلى 5 مجموعات: مجموعة السيطرة الطبيعية (A) ومجموعة المقرحة (B) تلقت طريق الفم 0.5 ٪ كاربوكسي ميثيل السليلوز (CMC)؛ (C) التي تناولت أوميبرازول 20 ملغم/كغم؛ المجموعتين D وE، 250 و500 ملغم/كغم EILE على التوالي. بعد 60 دقيقة، المجموعات B-E جرعة فموية المطلق لاحداث قرحة وبعد دقيقة أخرى، تخدير والتضحية بها. أظهرت تجربة عدم وجود أي علامات سمية الجرذان المعرضة لتناول 2 و5 غم / كغم الفم. وكشفت الوقاية اختراق كبير لأنسجة القرحة، كما يتضح انخفاض محتوى المخاط، وانخفاض درجة الحموضة المعدة، واخداث تمزقات شديدة الطبقة المخاطية. اظهرت عولجت بالأوميبرازول أو تأثيرا قليلا جدا تلف أنسجة مما يشير تأثير منخفض ملحوظ جروح بوساطة الإيثانول، تحسن مخاط ودرجة الحموضة، وخفض مساحات الوذمة، وتسرب الكريات البيض تحت جناسة أدى علاج زيادة كبيرة إنزيمات مضادات الأكسدة (SOD وCAT) MDA ملحوظ. علاوة ذلك، المعالجة بـ التعبير صبغة PAS ظهارة والغدد القاعدية (القاعدة والرقبة والبرزخ). يمكن أن تعزى التأثيرات المعوية لـ زيادتها الإيجابية لعوامل المختلفة، قد يؤكد صحتها كمضاف علاجي مستقبلي لقرحة التقييم الدوائي.

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Boron Prevents Oral Acrylamide Damage in Bone Marrow and Hematologic Parameters of Wistar Rats

Biological Trace Element Research, Journal Year: 2025, Volume and Issue: unknown

Published: April 7, 2025

Abstract The purpose of this study is to measure the effects acrylamide (AA) on bone marrow and blood protective efficacy boric acid (B) in rats. animals were divided into five groups: control, B, low-dose B + AA, high-dose AA. After 15 th day experiment, their harvested be kept for hematological analyses. While white cells (WBC) increased following administration bodyweight, red cells, platelets, hemoglobin, hematocrit, nucleated cell counts significantly decreased number. Conversely, bodyweight all these parameters apart from WBC AA groups when compared control. However, was more effective than preventing AA-induced damage. present suggests that treatment could help protect against toxicity. Graphical

Language: Английский

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Determination of the biological activities of newly synthesized and characterized 4-(4-methoxybenzo[d][1,3,2]dioxaborol-2-yl)phenyl)boronic acid (M86) and 1,4-bis(4-methoxybenzo[d][1,3,2]dioxaborol-2-yl)benzene (M87) compounds and evaluation of their protective activity in Lead, Cadmium and Arsenic toxicity in 2D and 3D model THLE-2 liver cell line

Journal of Trace Elements in Medicine and Biology, Journal Year: 2025, Volume and Issue: unknown, P. 127665 - 127665

Published: May 1, 2025

Language: Английский

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In Vitro Effects of Boric Acid on Cell Cycle, Apoptosis, and miRNAs in Medullary Thyroid Cancer Cells

Biological Trace Element Research, Journal Year: 2024, Volume and Issue: unknown

Published: April 30, 2024

Medullary thyroid cancer (MTC) is a highly aggressive and chemotherapy-resistant originating from the thyroid's parafollicular C cells. Due to its resistance conventional treatments, alternative therapies such as boric acid have been explored. Boric acid, boron-based compound, has shown anticarcinogenic effects, positioning it potential treatment option for MTC. TT medullary carcinoma cell line (TT cells) human fibroblast (HThF were utilized culture experiments. Cell viability was assessed using 2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide (XTT) assay. Total RNA extracted Trizol reagent gene expression microRNA (miRNA) analysis via reverse transcription-polymerase chain reaction (RT-PCR). The extent of apoptosis induced by determined terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) Colony formation assays conducted evaluate impact on colony-forming ability MTC At 48 h, 50% inhibitory concentration (IC50) found be 35 μM. Treatment with resulted in significant modulation apoptosis-related genes miRNAs, including increased phorbol-12-myristate-13-acetate-induced protein 1(NOXA), apoptotic protease activating factor 1 (APAF-1), Bcl-2-associated X (Bax), caspase-3, caspase-9. In contrast, B lymphoma 2 (Bcl2), lymphoma- extra-large (Bcl-xl), microRNA-21 (miR-21), which are linked aggressiveness MTC, significantly reduced. TUNEL assay indicated 14% rate, there 67.9% reduction colony formation, Our study suggests that may anticancer activity modulating pathways. These findings suggest could therapeutic agent possibly other malignancies similar pathogenic mechanisms.

Language: Английский

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Novel indole Schiff base β-diiminato compound as an anti-cancer agent against triple-negative breast cancer: In vitro anticancer activity evaluation and in vivo acute toxicity study

Bioorganic Chemistry, Journal Year: 2024, Volume and Issue: 152, P. 107730 - 107730

Published: Aug. 16, 2024

Breast cancer is the most prevalent among women globally, with triple-negative breast (TNBC) associated poor prognosis and low five-year survival rates. Schiff base compounds, known for their extensive pharmacological activities, have garnered significant attention in drug research. This study aimed to evaluate anticancer potential of a novel β-diiminato compound elucidate its mechanism action. The compound's effect on cell viability was assessed using MTT assays lines including MCF-7 MDA-MB-231. Cytotoxic effects were further analyzed trypan blue exclusion lactate dehydrogenase (LDH) release assays. In order assess inhibitory activity mode death induced by this compound, flow cytometry cycle distribution apoptosis analysis carried out. Apoptosis incidence initially through nuclear morphological changes (Hoechst 33342/Propidium iodide (PI) staining) confirmed Annexin V/PI staining analysis. addition, disruption mitochondrial membrane (MMP) generation reactive oxygen species (ROS) determined JC-1 indicator DCFDA dye, respectively. results demonstrated that 24 h treatment significantly suppressed MDA-MB-231 cells dose-dependent manner IC

Language: Английский

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