
Italian Journal of Animal Science, Journal Year: 2025, Volume and Issue: 24(1), P. 1046 - 1059
Published: April 30, 2025
Language: Английский
Italian Journal of Animal Science, Journal Year: 2025, Volume and Issue: 24(1), P. 1046 - 1059
Published: April 30, 2025
Language: Английский
Theriogenology, Journal Year: 2025, Volume and Issue: unknown, P. 117425 - 117425
Published: April 1, 2025
Language: Английский
Citations
1Reproduction in Domestic Animals, Journal Year: 2025, Volume and Issue: 60(4)
Published: April 1, 2025
ABSTRACT This study investigated the potential of incorporating curcumin oil nano‐emulsion (CONE) into a Tris‐based freezing extender to relieve cryodamage effects and enhance sperm cryo‐resistance in buffalo bulls. Pooled semen from five fertile bulls ( n = 40 ejaculates) was divided supplemented with 0.5% CONE (CONE0.5), 1% (CONE1), or without (CONE0, control), then extended packaged. Post‐thaw assessments included kinematic parameters, oxidative stress markers, apoptosis levels, microbial load, ultrastructural integrity, molecular docking interactions, conception rates. The CONE0.5 group exhibited significantly higher viability, progressive motility, membrane as well lower nitric oxide MDA compared CONE0 CONE1 groups p < 0.05), indicating reduced stress. Compared group, percentage viable early apoptotic sperm, late 0.05). Both CONE‐treated demonstrated post‐thaw total bacterial, spore‐forming coliform bacterial counts Conception rates were (80.0%) (68.0%) > Molecular analysis revealed strong binding affinities between caspase‐3 (−7.87 kcal/mol), HSP70 (−8.52 PRDX‐1 (−8.90 suggesting mechanisms action. In summary, inclusion cryoprotective additive media demonstrates for improving quality possible enhancement reproductive efficiency.
Language: Английский
Citations
0Italian Journal of Animal Science, Journal Year: 2025, Volume and Issue: 24(1), P. 1046 - 1059
Published: April 30, 2025
Language: Английский
Citations
0