Artemisinin alleviates cisplatin-induced damage in GC-1 spermatogonia through ER stress mechanisms DOI Creative Commons
Ran Lee,

Won-Young Lee,

Dong-Wook Kim

et al.

Heliyon, Journal Year: 2025, Volume and Issue: 11(4), P. e42579 - e42579

Published: Feb. 1, 2025

Artemisinin, a compound derived from Artemisia annua, is primarily utilized for malaria treatment. Its mechanism of action involves the rapid and effective inhibition protein synthesis in malarial parasites. Recently, artemisinin has garnered extensive research attention its anticancer, antioxidant, anti-inflammatory properties, as well potential role an adjuvant cancer Cisplatin commonly used anticancer agent; however, therapeutic benefits are accompanied by side effects that negatively impact male reproductive function. In this study, protective effect against cisplatin-induced cytotoxicity was investigated. Type B mouse spermatogonia (GC-1 spg cells), testes, were treated with various concentrations (10–200 μM) to identify optimal concentration promoting cell proliferation. induced antiproliferative apoptotic death GC-1 cells, whereas combination cisplatin restored proliferation reduced apoptosis. Treatment resulted elevated levels endoplasmic reticulum (ER) stress-related factors, such Bip/GRP78, PDI, Ero1-la, while effectively inhibited these levels. Additionally, increased inflammatory markers, including COX2, iNOS, NF-κB, which subsequently decreased artemisinin. This study evaluates artemisinin, naturally compound, mitigator on germ cells during cisplatin-based conclusion, findings suggest may serve supplement or functional agent therapy.Graphical abstract

Language: Английский

Artemisinin alleviates cisplatin-induced damage in GC-1 spermatogonia through ER stress mechanisms DOI Creative Commons
Ran Lee,

Won-Young Lee,

Dong-Wook Kim

et al.

Heliyon, Journal Year: 2025, Volume and Issue: 11(4), P. e42579 - e42579

Published: Feb. 1, 2025

Artemisinin, a compound derived from Artemisia annua, is primarily utilized for malaria treatment. Its mechanism of action involves the rapid and effective inhibition protein synthesis in malarial parasites. Recently, artemisinin has garnered extensive research attention its anticancer, antioxidant, anti-inflammatory properties, as well potential role an adjuvant cancer Cisplatin commonly used anticancer agent; however, therapeutic benefits are accompanied by side effects that negatively impact male reproductive function. In this study, protective effect against cisplatin-induced cytotoxicity was investigated. Type B mouse spermatogonia (GC-1 spg cells), testes, were treated with various concentrations (10–200 μM) to identify optimal concentration promoting cell proliferation. induced antiproliferative apoptotic death GC-1 cells, whereas combination cisplatin restored proliferation reduced apoptosis. Treatment resulted elevated levels endoplasmic reticulum (ER) stress-related factors, such Bip/GRP78, PDI, Ero1-la, while effectively inhibited these levels. Additionally, increased inflammatory markers, including COX2, iNOS, NF-κB, which subsequently decreased artemisinin. This study evaluates artemisinin, naturally compound, mitigator on germ cells during cisplatin-based conclusion, findings suggest may serve supplement or functional agent therapy.Graphical abstract

Language: Английский

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