Comprehensive analysis and experiment validation of five cuproptosis-related genes in prognosis, immune infiltration and metabolic characterization of pancreatic cancer DOI Creative Commons
Quantong Deng,

Kun Yang,

Qiaoling Liao

et al.

PLoS ONE, Journal Year: 2025, Volume and Issue: 20(5), P. e0323458 - e0323458

Published: May 14, 2025

Background Cuproposis is a new-found mechanism of cell death, and the role cuproposis-related genes (CRGs) in pancreatic cancer prognosis remains uncertain. Methods DECRGs were identified from TCGA GTEx databases. Five OS-associated hub screened using Cox regression LASSO analyses. A prognostic model was constructed validated by survival analysis. GSEA, gene mutation, small-molecule drugs, immune-infiltrating TF/miRNA/mRNA network investigated to determine underlying 5-CRGs. In addition, RT-qPCR, WB applied validate expression CCK8, colony formation transwell assays used prove function LIPT1 PC. Results PDP1, DLAT, DBT, LIAS, as genes. 5-CRGs established low-risk population has longer OS. There high risk score value for prediction clinicopathological features. The forest plots showed that age, N stage RiskScore significant independent indicators. T cells CD4 memory resting Mast are amplest immune subpopulations high-score individuals. 5 CRGs exhibited differences PC lines tissues, LIPT1-knockdowning inhibited proliferation invasion lines. Conclusion relevant identified. Meanwhile, new accurate five constructed. may promote proliferation, migration This have specific guiding future development precise anti-cancer treatment strategies.

Language: Английский

Comprehensive analysis and experiment validation of five cuproptosis-related genes in prognosis, immune infiltration and metabolic characterization of pancreatic cancer DOI Creative Commons
Quantong Deng,

Kun Yang,

Qiaoling Liao

et al.

PLoS ONE, Journal Year: 2025, Volume and Issue: 20(5), P. e0323458 - e0323458

Published: May 14, 2025

Background Cuproposis is a new-found mechanism of cell death, and the role cuproposis-related genes (CRGs) in pancreatic cancer prognosis remains uncertain. Methods DECRGs were identified from TCGA GTEx databases. Five OS-associated hub screened using Cox regression LASSO analyses. A prognostic model was constructed validated by survival analysis. GSEA, gene mutation, small-molecule drugs, immune-infiltrating TF/miRNA/mRNA network investigated to determine underlying 5-CRGs. In addition, RT-qPCR, WB applied validate expression CCK8, colony formation transwell assays used prove function LIPT1 PC. Results PDP1, DLAT, DBT, LIAS, as genes. 5-CRGs established low-risk population has longer OS. There high risk score value for prediction clinicopathological features. The forest plots showed that age, N stage RiskScore significant independent indicators. T cells CD4 memory resting Mast are amplest immune subpopulations high-score individuals. 5 CRGs exhibited differences PC lines tissues, LIPT1-knockdowning inhibited proliferation invasion lines. Conclusion relevant identified. Meanwhile, new accurate five constructed. may promote proliferation, migration This have specific guiding future development precise anti-cancer treatment strategies.

Language: Английский

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