RNA Sequencing in Disease Diagnosis

Annual Review of Genomics and Human Genetics, Journal Year: 2024, Volume and Issue: 25(1), P. 353 - 367

Published: Feb. 16, 2024

RNA sequencing (RNA-seq) enables the accurate measurement of multiple transcriptomic phenotypes for modeling impacts disease variants. Advances in technologies, experimental protocols, and analysis strategies are rapidly expanding application RNA-seq to identify biomarkers, tissue- cell-type-specific impacts, spatial localization disease-associated mechanisms. Ongoing international efforts construct biobank-scale repositories with matched genomic data across diverse population groups further increasing utility approaches by providing large-scale normative reference resources. The availability these resources, combined improved computational pipelines, has enabled detection aberrant underlying rare diseases. Further expansion both somatic developmental tissues, is expected soon provide unprecedented insights resolve origin, mechanism action, causal gene contributions, suggesting continued high diagnosis.

Language: Английский

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A review of the advances, insights, and prospects of gene therapy for Alzheimer’s disease: A novel target for therapeutic medicine

Gene, Journal Year: 2024, Volume and Issue: 912, P. 148368 - 148368

Published: March 12, 2024

Language: Английский

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Advances in physiological and clinical relevance of hiPSC-derived brain models for precision medicine pipelines

Frontiers in Cellular Neuroscience, Journal Year: 2025, Volume and Issue: 18

Published: Jan. 6, 2025

Precision, or personalized, medicine aims to stratify patients based on variable pathogenic signatures optimize the effectiveness of disease prevention and treatment. This approach is favorable in context brain disorders, which are often heterogeneous their pathophysiological features, patterns progression treatment response, resulting limited therapeutic standard-of-care. Here we highlight transformative role that human induced pluripotent stem cell (hiPSC)-derived neural models poised play advancing precision for particularly emerging innovations improve relevance hiPSC physiology. hiPSCs derived from accessible patient somatic cells can produce various types tissues; current efforts increase complexity these models, incorporating region-specific tissues non-neural microenvironment, providing increasingly relevant insights into human-specific neurobiology. Continued advances tissue engineering combined with genomics, high-throughput screening imaging strengthen physiological thus ability uncover mechanisms, vulnerabilities, fluid-based biomarkers will have real impact neurological True understanding, however, necessitates integration hiPSC-neural biophysical data, including quantitative neuroimaging representations. We discuss recent cellular neuroscience provide direct connections through generative AI modeling. Our focus great potential synergy between pave way personalized becoming a viable option suffering neuropathologies, rare epileptic neurodegenerative disorders.

Language: Английский

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Efficient and reproducible differentiation protocol for pluripotent stem cells into functional endothelial cells: Unveiling the path to vascular regeneration

Archives of Medical Research, Journal Year: 2025, Volume and Issue: 56(3), P. 103142 - 103142

Published: Jan. 20, 2025

Language: Английский

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iPSC models of mitochondrial diseases

Neurobiology of Disease, Journal Year: 2025, Volume and Issue: unknown, P. 106822 - 106822

Published: Jan. 1, 2025

Language: Английский

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Age-Related Neurodegenerative Diseases: A Stem Cell’s Perspective

Cells, Journal Year: 2025, Volume and Issue: 14(5), P. 347 - 347

Published: Feb. 27, 2025

Neurodegenerative diseases encompass a number of very heterogeneous disorders, primarily characterized by neuronal loss and concomitant decline in neurological function. Examples this type clinical condition are Alzheimer's Disease, Parkinson's Huntington's Disease Amyotrophic Lateral Sclerosis. Age has been identified as major risk the etiology these which explains their increased incidence developed countries. Unfortunately, despite continued intensive efforts, no cure yet found for any diseases; reliable markers that allow an early diagnosis disease identification key molecular events leading to onset progression lacking. Altered adult neurogenesis appears precede appearance severe symptoms. Given scarcity human samples considerable differences with model species, increasingly complex stem-cell-based models being developed. These shedding light on alterations contribute development, facilitating new targets providing screening platform testing candidate drugs. Moreover, secretome other promising features cell types explored, use them replacement cells high plasticity or co-adjuvant therapy combinatorial treatments.

Language: Английский

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hiPSC-Driven Organoid Construction and Application Prospects

Regenerative medicine and dentistry., Journal Year: 2025, Volume and Issue: unknown, P. 5 - 5

Published: March 21, 2025

Perspective hiPSC-Driven Organoid Construction and Application Prospects Bangheng Liu 1,2, Yulei Mu 2,3 Dong-An Wang 1,2,* 1 Department of Biomedical Engineering, Chinese University Hong Kong, Sha Tin, New Territories, Kong SAR 999077, China 2 Center for Neuromusculoskeletal Restorative Medicine, InnoHK, HKSTP, 3 City 83 Tat Chee Avenue, Kowloon, * Correspondence: [email protected] Received: 5 March 2025; Revised: 19 Accepted: 20 Published: 21 2025 Abstract: Induced pluripotent stem cell (iPSC)-derived organoid platforms can simulate various target tissues hold broad application prospects in personalized medicine, disease modeling, drug screening, organ transplantation, understanding development mechanisms. Currently, the human iPSC (hiPSC) organoids is gradually shifting towards Matrigel-free scaffold-free systems, promoting precise control over composition structure these systems establishing induction protocols specialized organoids. Researchers are also exploring construction multifunctional with complex structures material exchange channels through vascularization, segmented induction, assembly technologies, though further breakthroughs needed. In future, hiPSC expected to advance precision treatment, high-throughput module detection multi-organ integration, automation. Additionally, when combined large artificial intelligence models, there potential establish data medical platforms, providing support clinical decision-making. Moreover, AI anticipated foster collaboration rather than competition, coordinated growth field. For hiPSC-derived it crucial enhance ethical review framework balance radical scientific exploration conservative public attitudes. must optimize or develop new reduce genomic instability tumorigenic risks, while avoiding emergence non-target cells insufficient functional maturity.

Language: Английский

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The mutual impacts of stem cells and sleep: opportunities for improved stem cell therapy

Stem Cell Research & Therapy, Journal Year: 2025, Volume and Issue: 16(1)

Published: March 29, 2025

Abstract Sleep is an indispensable physiological function regulated by circadian rhythms, which influence the biological pathways and overall health of body. crucial for maintenance restoration bodily systems, disturbances can lead to various sleep disorders, impair both mental physical health. Treatment options these disorders encompass lifestyle modifications, psychotherapy, medications, therapies such as light therapy surgery. Not only deprivation has a significant impact on essential organs, but it also influences types stem cells in In this review, we explore connection between cells, highlighting how rhythms regulate cell activities that are vital tissue regeneration homeostasis. Disruptions hinder self-renewal, homing, proliferation, function, differentiation, thereby affecting We discuss transplantation their products may help improve quality affects behavior, implications therapies. Notably, while certain transplantations disrupt sleep, enhancing efficacy Finally, be utilized model offering valuable insights into underlying mechanisms.

Language: Английский

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Induced Pluripotent Stem Cells-Based Regenerative Therapies in Treating Human Aging-Related Functional Decline and Diseases

Cells, Journal Year: 2025, Volume and Issue: 14(8), P. 619 - 619

Published: April 21, 2025

A significant increase in life expectancy worldwide has resulted a growing aging population, accompanied by rise aging-related diseases that pose substantial societal, economic, and medical challenges. This trend prompted extensive efforts within many scientific communities to develop enhance therapies aimed at delaying processes, mitigating functional decline, addressing aging-associated extend health span. Research biology focused on unraveling various biochemical genetic pathways contributing changes, including genomic instability, telomere shortening, cellular senescence. The advent of induced pluripotent stem cells (iPSCs), derived through reprogramming human somatic cells, revolutionized disease modeling understanding humans the limitations conventional animal models primary cells. iPSCs offer advantages over other such as embryonic they can be obtained without need for embryo destruction are not restricted availability healthy donors or patients. These attributes position iPSC technology promising avenue deciphering mechanisms underlie associated diseases, well studying drug effects. Moreover, exhibit remarkable versatility differentiating into diverse cell types, making them tool personalized regenerative replacing aged damaged with healthy, equivalents. review explores breadth research iPSC-based their potential applications spectrum conditions.

Language: Английский

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Research models to study lewy body dementia

Molecular Neurodegeneration, Journal Year: 2025, Volume and Issue: 20(1)

Published: April 23, 2025

Abstract Lewy body dementia (LBD) encompasses neurodegenerative dementias characterized by cognitive fluctuations, visual hallucinations, and parkinsonism. Clinical differentiation of LBD from Alzheimer’s disease (AD) remains complex due to symptom overlap, yet approximately 25% cases are diagnosed as postmortem, primarily identified the presence α-synuclein aggregates, tau tangles, amyloid plaques. These pathological features position a comorbid condition both Parkinson’s (PD) AD, with over 50% exhibiting co-pathologies. LBD’s mixed pathology complicates development comprehensive models that reflect full spectrum etiological, clinical, features. While existing animal cellular have facilitated significant discoveries in PD AD research, they lack specificity capturing unique pathogenic mechanisms, limiting exploration therapeutic avenues for specifically. This review assesses widely used terms their relevance LBD, particularly focusing on ability replicate human assess treatment efficacy. Furthermore, we discuss potential modifications these advance understanding mechanisms propose innovative research directions aimed at developing enhanced face, predictive, construct validity.

Language: Английский

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