A clinician’s perspective: what tumor-organoid researchers ought to know

Organoid, Journal Year: 2025, Volume and Issue: 5, P. e1 - e1

Published: Jan. 25, 2025

Patient-derived tumor organoids (PDTOs) provide powerful platforms for modeling human tumors, offering insights into cancer biology and personalized therapy development. Incorporating clinical knowledge—such as staging, molecular profiling, treatment outcomes—enhances the relevance of PDTO research. Lung staging systems guide sample selection, thereby influencing organoid growth response studies. Clinical endpoints characteristics further align experiments with real-world therapeutic challenges patient care. Despite their potential, research faces in replicating heterogeneity microenvironment, both which are critical studying drug resistance efficacy. Addressing these complexities requires close collaboration between researchers clinicians to ensure that models accurately reflect course disease. This review provides information on integrating research, focusing aspects such endpoints, implications staging. By bridging gap basic oncology, aims enhancing translational value work. A clear understanding can support development more robust models, ultimately advancing strategies improving outcomes.

Language: Английский

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Single-cell light-sheet fluorescence 3D images of tumour-stroma spheroid multicultures

Scientific Data, Journal Year: 2025, Volume and Issue: 12(1)

Published: March 24, 2025

Abstract Spheroids are widely used in oncology for testing drugs, but models composed of a single cell line do not fully capture the complexity vivo tumours targeted by chemotherapy. Developing 3D vitro that better mimic tumour architecture is crucial step scientific community. To enable more reliable drug testing, we generated multiculture spheroids and analysed morphology distribution over time. This dataset first publicly available single-cell light-sheet fluorescence microscopy image collection comprising three different lines at time points. Specifically, created one cancer (melanoma, breast cancer, or osteosarcoma) alongside two stromal (fibroblasts endothelial cells). Then, acquired resolution images to analyse spheroid after 24, 48, 96 hours. The collection, whole annotations, extracted features further research can support development automated analysis models.

Language: Английский

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Organoid-Immune Cell Co-culture for Stable Live Imaging

Methods in molecular biology, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Language: Английский

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The application of organoids in investigating immune evasion in the microenvironment of gastric cancer and screening novel drug candidates

Molecular Cancer, Journal Year: 2025, Volume and Issue: 24(1)

Published: April 26, 2025

Gastric cancer (GC) is a prevalent digestive system tumor, the fifth most diagnosed worldwide, and leading cause of deaths. GC distinguished by its pronounced heterogeneity dynamically evolving tumor microenvironment (TME). The lack accurate disease models complicates understanding mechanisms impedes discovery novel drugs. A growing body evidence suggests that organoids, developed using organoid culture technology, preserve genetic, phenotypic, behavioral characteristics. organoids hold significant potential for predicting treatment responses in individual patients. This review provides comprehensive overview current clinical strategies GC, as well history, construction applications organoids. focus on role simulating TME to explore immune evasion intratumoral microbiota their guiding drug therapy facilitating screening. Furthermore, we summarize limitations underscore need continued technological advancements benefit both basic translational oncological research.

Language: Английский

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Shrinking Cancer Research Barriers: Crafting Accessible Tumor‐on‐Chip Device for Gene Silencing Assays

Advanced Engineering Materials, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 5, 2024

Tumor‐on‐chip (ToC) is crucial to bridge the gap between traditional cell culture experiments and in vivo models, allowing recreate an vivo‐like microenvironment cancer research. ToC use microfluidics provide fine‐tune control over environmental factors, high‐throughput screening, reduce requirements of samples reagents. However, creating these microfluidic devices requires skilled researchers dedicated manufacturing equipment, making widespread adoption cumbersome difficult. To address some bottlenecks improve accessibility technology, innovative materials fabrication processes are required. Polystyrene (PS) a promising material for due its biocompatibility, affordability, optical transparency. Herein, process based on direct laser writing thermosensitive PS, swift economical crafting with easy pattern alterations, presented. For first time, device fabricated only by PS presented, customizing optimization efficient approaches. These biochips support 2D 3D cultures comparable viability proliferation kinetics 96‐well plates. The data show that gene protein silencing efficiencies remain consistent across both chip plate‐based cultures, either or spheroid format. Although simple, this approach might facilitate customized chip‐based models.

Language: Английский

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A multiparametric analysis including single-cell and subcellular feature assessment reveals differential behavior of spheroid cultures on distinct ultra-low attachment plate types

Frontiers in Bioengineering and Biotechnology, Journal Year: 2024, Volume and Issue: 12

Published: Aug. 2, 2024

Spheroids have become principal three-dimensional models to study cancer, developmental processes, and drug efficacy. Single-cell analysis techniques emerged as ideal tools gauge the complexity of cellular responses in these models. However, single-cell quantitative assessment based on 3D-microscopic data subcellular distribution fluorescence markers, such nuclear/cytoplasm ratio transcription factors, has largely remained elusive. For spheroid generation, ultra-low attachment plates are noteworthy due their simplicity, compatibility with automation, experimental commercial accessibility. it is unknown whether what degree plate type impacts formation biology. This developed a novel AI-based pipeline for 3D-confocal optically cleared large spheroids at wholemount, single-cell, sub-cellular levels. To identify relevant samples pipeline, automated brightfield microscopy was employed systematically compare size eccentricity formed six different types using four distinct human cell lines. showed that all exhibited similar spheroid-forming capabilities gross patterns growth or shrinkage during 4 days after seeding were comparable. Yet, varied among specific lines types. Based this prescreen, HaCaT keratinocytes HT-29 cancer cells further assessed. In spheroids, in-depth revealed correlation between size, proliferation, transcriptional coactivator, YAP1, well an inverse respect differentiation. These findings, yielded model level, corroborate earlier concepts role YAP1 proliferation differentiation skin. Further, results show may influence outcome campaigns advisable scan optimal configuration investigation.

Language: Английский

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Organoids research progress in gynecological cancers: a bibliometric analysis

Frontiers in Oncology, Journal Year: 2024, Volume and Issue: 14

Published: Oct. 28, 2024

Background Gynecological cancers (GC) pose a severe threat to the health and safety of women’s lives, organoids, as in-vitro research models, have demonstrated significant advantages in simulating tissue characteristics drug screening. In recent years, there has been rapid increase outcomes related organoids GC. However, no bibliometric study concerning. Methods Publications GC from 2010-2023 were retrieved Web Science Core Collection (WoSCC). We conducted analysis visualization using CiteSpace, VOSviewer, Bibliometrix R Package. This included spatiotemporal distribution, author, sources, references, keywords. Results A total 333 publications included. The number annual indicated an explosive phase development since 2019. USA was most important country terms cooperation, publication output, citation centrality. University California system ranked first productivity among institutions, HIPPO Y is relevant author field. CANCERS published documents, NATURE cited sources. Analysis Keywords References, it possible establish trend, find hotspots Conclusion delineated global landscapes progress trends research. emphasized that can effectively replicate original or tumors, providing good model for on tumor-related mechanisms showing screening efficacy clinical prediction. Additionally, preclinical they provide compelling evidence personalized therapy prediction patient responses.

Language: Английский

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The new platform for cancer immunity research: Organoid systems

Elsevier eBooks, Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 1, 2024

Language: Английский

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Advancements in 3D Cell Culture Models for Drug Discovery and Disease Modeling

Next frontier., Journal Year: 2024, Volume and Issue: 8(1), P. 203 - 203

Published: Nov. 28, 2024

The advent of 3D cell culture models has revolutionized drug discovery and disease modeling, offering more physiologically relevant systems compared to traditional 2D cultures. These advanced mimic the in vivo microenvironment, incorporating critical cellular interactions, extracellular matrix components, gradients oxygen nutrients. This research explores development application technologies, such as organoids, spheroids, bioprinted tissues, studying mechanisms evaluating efficacy. study highlights advantages these recapitulating complex biological processes, including tumor microenvironments, organ development, tissue-specific responses therapeutic agents. By employing cutting-edge techniques microfluidics, imaging, computational aims optimize for high-throughput screening personalized medicine. findings underscore transformative potential bridging gap between preclinical studies clinical outcomes, reducing costs improving patient-specific approaches.

Language: Английский

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