Assessment of the Dose-Dependent Effect of Human Platelet Lysate on Wharton’s Jelly-Derived Mesenchymal Stem/Stromal Cells Culture for Manufacturing Protocols DOI Creative Commons
Aleksandra Bzinkowska, Anna Sarnowska

Stem Cells and Cloning Advances and Applications, Journal Year: 2024, Volume and Issue: Volume 17, P. 21 - 32

Published: Oct. 1, 2024

Mesenchymal stem/stromal cells (MSCs)-based products have unique characteristics compared to other drugs because of their inherently variable effects depending on culture conditions and microenvironment. In some cases, can be produced individually, one batch at a time, for personalized therapy. Therefore, it is very important optimize both medium composition under Good Manufacturing Practice (GMP) standards. MSCs properties been exploited as potential cell therapies in regenerative medicine. The main mechanism protective effect based secretory activity. Simultaneously, secretome highly sensitive any change environmental conditions. Depending the type damage target application, desirable enhance secretion therapeutic factors. Changes modulation affect survival, migration ability, proliferative clonogenic potentials.

Language: Английский

Stem Cell Therapies in Canine Cardiology: Comparative Efficacy, Emerging Trends, and Clinical Integration DOI Creative Commons
Ahmed Farag, Hanan Hendawy, Mahmoud H. Emam

et al.

Biomolecules, Journal Year: 2025, Volume and Issue: 15(3), P. 371 - 371

Published: March 4, 2025

Cardiovascular diseases are a leading cause of morbidity and mortality in dogs, with limited options available for reversing myocardial damage. Stem cell therapies have shown significant potential cardiac repair, owing to their immunomodulatory, antifibrotic, regenerative properties. This review evaluates the therapeutic applications mesenchymal stem cells (MSCs) derived from bone marrow, adipose tissue, Wharton’s jelly focus on role canine cardiology immunoregulatory Preclinical studies highlighted efficacy enhancing function, reducing fibrosis, promoting angiogenesis. Various delivery methods, including intracoronary intramyocardial injections, assessed safety efficacy. Challenges such as low retention, differentiation efficiency, variability responses also discussed. Emerging strategies, genetic modifications combination therapies, aim enhance MSCs. Additionally, advances systems regulatory frameworks reviewed support clinical translation. comprehensive evaluation underscores revolutionize cardiovascular disease management while identifying critical areas future research integration.

Language: Английский

Citations

1
The Exosomes Derived From Bone Marrow Mesenchymal Stem Cells Alleviate Inflammatory Injury in Heart Failure Disease by Enhancing the Expression of KLF4 DOI Creative Commons

Yutong Han,

Yang Bi,

Zhang Dong-Hai

et al.

Immunity Inflammation and Disease, Journal Year: 2025, Volume and Issue: 13(3)

Published: March 1, 2025

The aim of this study is to investigate the impact and mechanism action exosomes derived from bone marrow mesenchymal stem cells (BMSCs) in treatment heart failure (HF). analysis gene sequencing data set was employed identify potential therapeutic target proteins for HF. Subsequently, H9C2 Sprague-Dawley (SD) rats were utilized as experimental models simulate doxorubicin hydrochloride (Doxorubicin, Dox)-induced myocardial injury. This approach expression changes inflammatory factors, including TNF-α, IL-6, IL-1β, sST2, Gal-3, well alterations their following exosome treatment. Meanwhile, relieving HF inhibiting inflammation investigated using constructed KLF 4 knockout cell lines SD rats. BMSC-derived capable enhancing level KLF4 cardiomyocytes, decreasing levels damage markers BNP hs-TnI, factors thereby alleviating In vitro vivo experiments have shown that exosomal decreases which are indicators injury, along with release cytokines cardiomyocytes. Concurrently, downregulated, leading a significant reduction physiological modulation. exhibit superior by mitigating tissue.

Language: Английский

Citations

0
Genotype–phenotype correlations in inherited cardiomyopathies, their role in clinical decision-making, and implications in personalized cardiac medicine in multi-omics as well as disease modeling eras DOI Creative Commons

Yaqob Samir Taleb,

Paras Memon,

Aftab Ahmed Jalbani

et al.

Saudi Journal for Health Sciences, Journal Year: 2025, Volume and Issue: 14(1), P. 30 - 41

Published: Jan. 1, 2025

Inherited cardiomyopathies are a diverse group of heart muscle diseases caused by genetic mutations that result in structural and functional abnormalities the myocardium. Understanding genotype–phenotype correlations these conditions is vital for personalized cardiac medicine, enabling targeted therapeutic strategies predictive diagnostics. This review explores major types inherited cardiomyopathies–hypertrophic cardiomyopathy, dilated arrhythmogenic restrictive cardiomyopathy–and provides detailed insights into how different manifest as clinical features. The integration multi-omics approaches advanced disease modeling techniques has enhanced our ability to dissect correlations. also discusses implications findings including tailored strategies, diagnostics, future research directions. JOURNAL/sjfhs/04.03/01772839-202501000-00004/figure1/v/2025-04-19T121403Z/r/image-tiff

Language: Английский

Citations

0
Mesenchymal Stem Cell Secretome Restores Monocrotophos Induced Toxicity in Human Neural Progenitor Cells DOI
Pankhi Vatsa, Anand Srivastava,

Amita Srivastava

et al.

Biochemical and Biophysical Research Communications, Journal Year: 2025, Volume and Issue: unknown, P. 151987 - 151987

Published: May 1, 2025

Language: Английский

Citations

0
Genotype-Phenotype Correlations in Inherited Cardiomyopathies, Their Role in Clinical Decision-Making and Implications in Personalized Cardiac Medicine in Multi-omics as Well as Disease Modelling Eras DOI Open Access

Yaqob Samir Taleb,

Paras Memon,

Aftab Ahmed Jalbani

et al.

Published: Aug. 9, 2024

Inherited cardiomyopathies are a diverse group of heart muscle diseases caused by genetic mutations that result in structural and functional abnormalities the myocardium. Understanding genotype-phenotype correlations these conditions is vital for personalized cardiac medicine, enabling targeted therapeutic strategies predictive diagnostics. This review explores major types inherited cardiomyopathies—hypertrophic cardiomyopathy (HCM), dilated (DCM), arrhythmogenic right ventricular (ARVC), restrictive (RCM)—and provides detailed insights into how different manifest as clinical features. The integration multi-omics approaches advanced disease modeling techniques has enhanced our ability to dissect correlations. also discusses implications findings including tailored strategies, diagnostics, future research directions.

Language: Английский

Citations

1
Assessment of the Dose-Dependent Effect of Human Platelet Lysate on Wharton’s Jelly-Derived Mesenchymal Stem/Stromal Cells Culture for Manufacturing Protocols DOI Creative Commons
Aleksandra Bzinkowska, Anna Sarnowska

Stem Cells and Cloning Advances and Applications, Journal Year: 2024, Volume and Issue: Volume 17, P. 21 - 32

Published: Oct. 1, 2024

Mesenchymal stem/stromal cells (MSCs)-based products have unique characteristics compared to other drugs because of their inherently variable effects depending on culture conditions and microenvironment. In some cases, can be produced individually, one batch at a time, for personalized therapy. Therefore, it is very important optimize both medium composition under Good Manufacturing Practice (GMP) standards. MSCs properties been exploited as potential cell therapies in regenerative medicine. The main mechanism protective effect based secretory activity. Simultaneously, secretome highly sensitive any change environmental conditions. Depending the type damage target application, desirable enhance secretion therapeutic factors. Changes modulation affect survival, migration ability, proliferative clonogenic potentials.

Language: Английский

Citations

0