Evaluation of the Anti-Inflammatory/Immunomodulatory Effect of Teucrium montanum L. Extract in Collagen-Induced Arthritis in Rats DOI Creative Commons
Biljana Bufan, Mirjana Marčetić, Jasmina Djuretić

et al.

Biology, Journal Year: 2024, Volume and Issue: 13(10), P. 818 - 818

Published: Oct. 12, 2024

The anti-inflammatory/immunomodulatory effects of Teucrium montanum L. (TM), a plant distributed in the Mediterranean region, have been insufficiently examined. TM ethanol extract were tested rat collagen-induced arthritis (CIA) model rheumatoid arthritis. LC-MS was used for phytochemical analysis extract. Dark Agouti rats immunized with bovine type II collagen (CII) incomplete Freund’s adjuvant CIA, and treated 100 or 200 mg/kg daily via oral administration. Clinical histopathological evaluations flow cytometric phenotypic functional characteristics splenocytes draining lymph node cells performed. cytokines paw tissue culture supernatants anti-CII antibodies serum determined by ELISA. extract, dominant components verbascoside luteolin 7-O-rutinoside, reduced arthritic score ankle joint inflammation CIA rats, promoted antioxidant profile serum, lowered pro-inflammatory TNF-α, IL-6 IL-1β production. It suppressed activation status CD11b+ lowering CD86, MHCII TLR-4 expression, Th17/T regulatory cell (Tregs) balance towards Tregs. A lower frequency B accompanied level rats. These findings imply favorable effect on clinical presentation suggesting its action potential therapeutic effect.

Language: Английский

Natural polysaccharide-based nanodrug delivery systems for targeted treatment of rheumatoid arthritis: A review DOI

Devesh Kumar,

Shubham Pandey,

Brijesh Shivhare

et al.

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: unknown, P. 143408 - 143408

Published: April 1, 2025

Language: Английский

Citations

0
Unveiling the mechanism of amelioration of adjuvant-induced rheumatoid arthritis by Drynaria quercifolia rhizome extract using network pharmacology and gene expression-based studies DOI Creative Commons
Debabrata Modak, Subhajit Ghosh, Sourav Sarkar

et al.

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: April 8, 2025

Rhizomes of Drynaria quercifolia have long been traditionally used to manage rheumatic pain. However, there is limited research supporting this traditional practice and insufficient evidence demonstrating the molecular mechanisms action plant-derived bioactives in rheumatoid arthritis (RA). The current study aims identify effective components methanol rhizome extract (DME) their probable pharmacological alleviating Rheumatoid Arthritis (RA) using network-pharmacology, docking, molecular-dynamics simulations, gene expression-based validation. Gas chromatography-mass spectrometry (GC-MS) based screening identified 41 volatile phytocomponents from DME having drug-like potentiality. Network pharmacology-based revealed 117 therapeutic targets for RA which 11 as core targets. Gene Ontology (GO) Kyoto Encyclopedia Genes Genomes (KEGG) enrichment analysis indicated that key target genes were mostly enriched inflammatory response associated with multiple signalling pathways. Molecular docking dynamics studies proteins like serine/threonine-protein kinase (AKT1), peroxisome proliferator-activated receptor alpha (PPARA), gamma (PPARG), exhibited strong binding affinity stable interactions present DME. For experimental verification FCA (Freund's complete adjuvant)-induced chronic model employed further investigation. Quantitative reverse transcription polymerase chain reaction (qRT-PCR) results validated significantly (p ≤ 0.05) regulate expression AKT PPARG model. Moreover, confirmed downregulated pro-inflammatory mediators COX-2, IL-6 TNF-α at protein levels also normalized different oxidative stress parameters both low high dose groups DME-treated arthritic animals. In conclusion, network-based silico approach probably act a synergistic way modulate RA, was by studies. Therefore, could be potential alternative immunomodulatory therapies combat related conditions.

Language: Английский

Citations

0
New Opportunities for Examining Natural and Traditional Herbal Medicines in Rheumatoid Arthritis DOI Creative Commons

Kristina A. Perez,

Jonathan W. Lowery

Deleted Journal, Journal Year: 2024, Volume and Issue: 22(1)

Published: May 23, 2024

Language: Английский

Citations

0
Evaluation of the Anti-Inflammatory/Immunomodulatory Effect of Teucrium montanum L. Extract in Collagen-Induced Arthritis in Rats DOI Creative Commons
Biljana Bufan, Mirjana Marčetić, Jasmina Djuretić

et al.

Biology, Journal Year: 2024, Volume and Issue: 13(10), P. 818 - 818

Published: Oct. 12, 2024

The anti-inflammatory/immunomodulatory effects of Teucrium montanum L. (TM), a plant distributed in the Mediterranean region, have been insufficiently examined. TM ethanol extract were tested rat collagen-induced arthritis (CIA) model rheumatoid arthritis. LC-MS was used for phytochemical analysis extract. Dark Agouti rats immunized with bovine type II collagen (CII) incomplete Freund’s adjuvant CIA, and treated 100 or 200 mg/kg daily via oral administration. Clinical histopathological evaluations flow cytometric phenotypic functional characteristics splenocytes draining lymph node cells performed. cytokines paw tissue culture supernatants anti-CII antibodies serum determined by ELISA. extract, dominant components verbascoside luteolin 7-O-rutinoside, reduced arthritic score ankle joint inflammation CIA rats, promoted antioxidant profile serum, lowered pro-inflammatory TNF-α, IL-6 IL-1β production. It suppressed activation status CD11b+ lowering CD86, MHCII TLR-4 expression, Th17/T regulatory cell (Tregs) balance towards Tregs. A lower frequency B accompanied level rats. These findings imply favorable effect on clinical presentation suggesting its action potential therapeutic effect.

Language: Английский

Citations

0