circHOMER1 Alleviates Sevoflurane‐Induced Hippocampal Neuronal Injury via Targeted Negative Regulation of miR‐217

Journal of Biochemical and Molecular Toxicology, Journal Year: 2025, Volume and Issue: 39(1)

Published: Jan. 1, 2025

ABSTRACT Sevoflurane (Sev) is a widely applied anesthetic in clinical practice; however, it could induce neurotoxicity and lead to postoperative cognitive dysfunction (POCD). This study aimed investigate the role underlying mechanism of circHOMER1 Sev‐induced POCD. Sev treated mouse hippocampal neuronal HT22 cells SD rats. RT‐qPCR was used detect levels miR‐217. ELISA employed measure inflammatory factors IL‐6, IL‐1β, TNF‐α. Commercially available kits assessed concentration MDA measured activities LDH SOD. The CCK‐8 assay cell viability. Flow cytometry analyzed apoptosis. Morris water maze test evaluated learning abilities Dual luciferase reporter assays RIP experiments validated targeted binding treatment significantly reduces viability, increases apoptosis, stimulates responses oxidative stress, induces memory impairments Following exposure Sev, expression markedly decreased, while overexpression can alleviate neuroinflammation, deficits CircHOMER1 targets miR‐217, transfection miR‐217 antagonizes neuroprotective effects circHOMER1. demonstrated that negatively regulated thereby inhibiting disorders.

Language: Английский

Sevoflurane-Induced Diffuse Alveolar Hemorrhage: A Descriptive Analysis of All Cases Reported in the Literature

Current anesthesiology reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: March 8, 2025

Language: Английский