Differentially Expressed Genes in Rat Brain Regions with Different Degrees of Ischemic Damage

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(5), P. 2347 - 2347

Published: March 6, 2025

Ischemic stroke is a multifactorial disease that leads to brain tissue damage and severe neurological deficit. Transient middle cerebral artery occlusion (tMCAO) models are actively used for the molecular, genetic study of stroke. Previously, using high-throughput RNA sequencing (RNA-Seq), we revealed 3774 differentially expressed genes (DEGs) in penumbra-associated region frontal cortex (FC) rats 24 h after applying tMCAO model. Here, studied gene expression pattern striatum contained an ischemic focus. Striatum samples were obtained from same which previously FC samples. Therefore, compared DEG profiles between two rat tissues tMCAO. Tissues selected based on magnetic resonance imaging (MRI) histological examination (HE) data. As result, 4409 DEGs identified striatum. Among them, 2609 overlapped FC, whereas more than one thousand specific each tissue. Furthermore, 54 exhibited opposite changes at mRNA level Thus, spatial regulation process ipsilateral hemisphere transcriptome was revealed. We believe targeted adjustment genome responses can be key induction regeneration processes cells

Language: Английский

Identification and Validation of Glycosylation‑Related Genes in Ischemic Stroke Based on Bioinformatics and Machine Learning

Journal of Molecular Neuroscience, Journal Year: 2025, Volume and Issue: 75(2)

Published: April 29, 2025

Language: Английский