Characterization of residual cancer by comparison of a pair of organoids established from a patient with ESCC before and after neoadjuvant chemotherapy DOI Creative Commons

Takafumi Fuchino,

Shusaku Kurogi,

Yoshiyuki Tsukamoto

et al.

Research Square (Research Square), Journal Year: 2023, Volume and Issue: unknown

Published: Aug. 17, 2023

Abstract Neoadjuvant chemotherapy (NAC) followed by surgery is a standard approach for management of locally advanced esophageal squamous cell carcinoma (ESCC). Patients who do not respond well to NAC have poor prognosis. Despite extensive research, the mechanisms chemoresistance in ESCC remain largely unknown. Here, we established paired tumor organoids – designated as PreNAC-O and PostNAC-O from one patient before after NAC, respectively. Although two did exhibit significant differences proliferation, morphology or drug sensitivity vitro, tumorigenicity vivo was significantly higher than that PreNAC-O. Xenografts tended keratinization, while those displayed conspicuous necrotic areas. The xenografts during comparable without treatment. Furthermore, gene expression profiles suggested genes involved EMT and/or hypoxia response might be related PostNAC-O. Our data residual cancer had been enhanced, outweighing effects chemotherapy, rather being attributable intrinsic chemoresistance. Further studies are required clarify extent which cancers share common mechanism similar revealed here.

Language: Английский

Molecular mechanisms and clinical significance of perineural invasion in malignancies: the pivotal role of tumor-associated Schwann cells in cancer progression and metastasis DOI
Noura A. A. Ebrahim,

Soliman M. A. Soliman,

Maha Othman

et al.

Medical Oncology, Journal Year: 2025, Volume and Issue: 42(5)

Published: April 21, 2025

Language: Английский

Citations

0
Selective targeting or reprogramming of intra-tumoral Tregs DOI
Keywan Mortezaee

Medical Oncology, Journal Year: 2024, Volume and Issue: 41(3)

Published: Feb. 11, 2024

Language: Английский

Citations

2
Ciliated, Mitochondria-Rich Postmitotic Cells are Immune-privileged, and Mimic Immunosuppressive Microenvironment of Tumor-Initiating Stem Cells: From Molecular Anatomy to Molecular Pathway DOI Creative Commons
Babak Behnam, Hassan Fazilaty,

M Ghadyani

et al.

Frontiers in Bioscience-Landmark, Journal Year: 2023, Volume and Issue: 28(10)

Published: Oct. 24, 2023

Cancer whose major problems are metastasis, treatment resistance, and recurrence is the leading cause of death worldwide. Tumor-initiating stem cells (TiSCs) a subset tumor population responsible for resistance relapse. Understanding characteristics shared features between tumor-initiating long-lived postmitotic may hold key to better understanding biology cancer. Postmitotic have exited cell cycle transitioned into non-dividing terminally differentiated state with specialized function within tissue. Conversely, cancer TiSC feature can divide produce variety progenies, disease progression, therapy immune system Surprisingly, our comprehensive evaluation TiSCs suggests common post-mitotic cells. They similar in structure (primary cilia, high mitochondrial content, being protected by barrier), metabolism (autophagy senescence), (immunoescape and/or immune-privileged blood barrier). In-depth exploration showed how contributes these features, including energy demands arising from ciliary microtubular functionality, increased metabolic activity, movement. These findings assist decoding remaining properties which offer insights TiSCs, potential implications enhancing strategies patient prognosis.

Language: Английский

Citations

5
Characterization of residual cancer by comparison of a pair of organoids established from a patient with esophageal squamous cell carcinoma before and after neoadjuvant chemotherapy DOI

Takafumi Fuchino,

Shusaku Kurogi,

Yoshiyuki Tsukamoto

et al.

Human Cell, Journal Year: 2024, Volume and Issue: 37(2), P. 491 - 501

Published: Jan. 6, 2024

Language: Английский

Citations

0
Exploring Oncogenic Factors Influence on Multiple Myeloma ogression and Patient Survival DOI
Muhammad Zahoor Khan, Adnan Khan, Ibrar Muhammad Khan

et al.

Diseases & Research, Journal Year: 2024, Volume and Issue: 4(0), P. 1 - 7

Published: Jan. 1, 2024

Language: Английский

Citations

0
Pharmacological HIF-1 activation upregulates extracellular vesicle production synergistically with adiponectin through transcriptional induction and protein stabilization of T-cadherin DOI Creative Commons

Kohei Fujii,

Yuya Fujishima, Shunbun Kita

et al.

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: Feb. 13, 2024

Abstract Pharmacological activation of hypoxia-inducible factor 1 (HIF-1), a hypoxia-responsive transcription factor, has attracted increasing attention due to its efficacy not only in renal anemia but also various disease models. Our study demonstrated that HIF-1 activator enhanced extracellular vesicle (EV) production from cultured endothelial cells synergistically with adiponectin, an adipocyte-derived through both transcriptional induction and posttranscriptional stabilization adiponectin binding partner, T-cadherin. Increased EV levels were observed wild-type mice T-cadherin null after consecutive administration roxadustat. Adiponectin- T-cadherin-dependent increased may be involved the pleiotropic effects activators.

Language: Английский

Citations

0
Characterization of residual cancer by comparison of a pair of organoids established from a patient with ESCC before and after neoadjuvant chemotherapy DOI Creative Commons

Takafumi Fuchino,

Shusaku Kurogi,

Yoshiyuki Tsukamoto

et al.

Research Square (Research Square), Journal Year: 2023, Volume and Issue: unknown

Published: Aug. 17, 2023

Abstract Neoadjuvant chemotherapy (NAC) followed by surgery is a standard approach for management of locally advanced esophageal squamous cell carcinoma (ESCC). Patients who do not respond well to NAC have poor prognosis. Despite extensive research, the mechanisms chemoresistance in ESCC remain largely unknown. Here, we established paired tumor organoids – designated as PreNAC-O and PostNAC-O from one patient before after NAC, respectively. Although two did exhibit significant differences proliferation, morphology or drug sensitivity vitro, tumorigenicity vivo was significantly higher than that PreNAC-O. Xenografts tended keratinization, while those displayed conspicuous necrotic areas. The xenografts during comparable without treatment. Furthermore, gene expression profiles suggested genes involved EMT and/or hypoxia response might be related PostNAC-O. Our data residual cancer had been enhanced, outweighing effects chemotherapy, rather being attributable intrinsic chemoresistance. Further studies are required clarify extent which cancers share common mechanism similar revealed here.

Language: Английский

Citations

0