The effect of miR-20b on breast cancer cell proliferation and related mechanism DOI Creative Commons
Wěi Li,

Pengjuan He,

Xiaochen Cui

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: May 23, 2024

Abstract Background and Purpose Breast cancer is the most common malignant tumor of women, contributes to main cause death female tumors. Although there are many mutated oncogenes anti-oncogenes during course breast formation, it not clear that specific molecular mechanism maintain proliferation cells. Studies have shown microRNAs (miRNAs) display expression in different tissues play an important role development The purpose this paper explore influences miR-20b on cell regulation function targeted genes.It will provide evidence for diagnosis treatment lay a foundation its clinical treatment. Methods We analyzed expressive levels within tissue corresponding normal adjacent carcinoma 10 patients, as well epithelial lines MCF-10A SKBR3, MCF-7, MDA-MB-231 through quantitative PCR (qPCR). line was up-regulated by miR-20b, effect ability cells were detected MTT colony formation assay. 231 model BALB/c mice. we used bioinformatics predict possible downstream target genes, verified luciferase experiment. Finally, genes qPCR western blot mRNA protein levels. Results present study confirmed level significantly reduced data showed inhibited cells, nude mice experiment hypoxia inducible factor-α(HIF-α)was potential gene miR-20b. Finally found could inhibit HIF-α Conclusions MiR-20b can

Language: Английский

Silymarin: a promising modulator of apoptosis and survival signaling in cancer DOI Creative Commons
Ujjawal Sharma,

Praveen Kumar Sahni,

Bunty Sharma

et al.

Discover Oncology, Journal Year: 2025, Volume and Issue: 16(1)

Published: Jan. 21, 2025

Abstract Cancer, one of the deadliest diseases, has remained epicenter biological research for more than seven decades. Yet all efforts a perfect therapeutic cure come with certain limitations. The use medicinal plants and their phytochemicals as therapeutics received much attention in recent years. Silymarin, polyphenolic flavonoid variety anti-cancerous properties, was isolated from plant Silybum marianum . present review centres on function silymarin controlling important signalling pathways related to apoptosis survival, such JAK/STAT pathway, PI3K/Akt/mTOR, Bcl-2/Bax, Fas/FasL. It is emphasised that silymarin's capacity target these key mechanism behind its anticancer effects against malignancies. By upregulating pro-apoptotic downregulating anti-apoptotic proteins, controls series events result tumor suppression cell death cancer types. low bioavailability limited efficacy are improved by application various nano-delivery systems. As efficient carriers, liposomes, polymeric micelles, lipid- metal-based nanoparticles, increase solubility distribution tissues. Lastly, number preclinical studies provide basis upcoming interventions highlighted review, providing encouraging directions additional advancement.

Language: Английский

Citations

1
Anticancer therapeutic potential of silibinin: current trends, scope and relevance DOI
Anupam Sharma, Sunil Kumar,

Virender Singh PAHIL

et al.

Medicinal Chemistry Research, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 5, 2025

Language: Английский

Citations

0
piRNAs and miRNAs Can Bind to mRNA of <i>KLOTHO</i> and <i>FGF23</i> Genes DOI Open Access
Ivashchenko At,

Kyrmyzy Akhmetova,

Murat Zhanuzakov

et al.

Published: June 14, 2023

The problem of increasing life expectancy is solved with the help many medical and social areas. It has been established that piRNAs miRNAs can significantly modify expression protein-coding genes by suppressing translation process. aim this work was to establish possibility binding mRNA KLOTHO FGF23 genes, which promote health increase through participation in key metabolic processes. We used MirTarget program, determines quantitative characteristics complementary interactions all nucleotides genes. piR-44682, piR-1940042, piR-3008660, piR-3215034, piR-6885965, piR-7980636 one miRNA (ID00756.3p-miR) gene were found cluster sites (BSs). piRNA-6890096 interacted a fully manner using only canonical nucleotides. Among 17494 human target interacting five bind identified. AFF2, BCL2L11, CPT1A, DAZAP1, NDRG3, SKIDA1, WBP4, ZIC5, ZSWIM6 piR-3215034 formed clusters BSs located 5'UTR, CDS 3'UTR. piR-576442, piR-1501557, piR-1845735, piR-2069834, piR-3029987 had mRNAs gene, proposed use as regulators anti-aging

Language: Английский

Citations

1
The effect of miR-20b on breast cancer cell proliferation and related mechanism DOI Creative Commons
Wěi Li,

Pengjuan He,

Xiaochen Cui

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: May 23, 2024

Abstract Background and Purpose Breast cancer is the most common malignant tumor of women, contributes to main cause death female tumors. Although there are many mutated oncogenes anti-oncogenes during course breast formation, it not clear that specific molecular mechanism maintain proliferation cells. Studies have shown microRNAs (miRNAs) display expression in different tissues play an important role development The purpose this paper explore influences miR-20b on cell regulation function targeted genes.It will provide evidence for diagnosis treatment lay a foundation its clinical treatment. Methods We analyzed expressive levels within tissue corresponding normal adjacent carcinoma 10 patients, as well epithelial lines MCF-10A SKBR3, MCF-7, MDA-MB-231 through quantitative PCR (qPCR). line was up-regulated by miR-20b, effect ability cells were detected MTT colony formation assay. 231 model BALB/c mice. we used bioinformatics predict possible downstream target genes, verified luciferase experiment. Finally, genes qPCR western blot mRNA protein levels. Results present study confirmed level significantly reduced data showed inhibited cells, nude mice experiment hypoxia inducible factor-α(HIF-α)was potential gene miR-20b. Finally found could inhibit HIF-α Conclusions MiR-20b can

Language: Английский

Citations

0