Single‐Cell Transcriptomics Uncovers Core Signature for Regulating Mitochondrial Homeostasis During Testicular Ageing DOI Creative Commons
Weijie Xu,

Qiuru Huang,

Yujuan Qi

et al.

Cell Proliferation, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 27, 2024

ABSTRACT Testicular ageing is accompanied by a series of morphological changes, while the features mitochondrial dysfunction remain largely unknown. Herein, we observed range age‐related modifications in testicular morphology and spermatogenic cells, conducted single‐cell RNA sequencing on young old testes Drosophila . Pseudotime trajectory revealed significant changes germline subpopulations during ageing. Our examination unveiled that genes showing bias spermatids exhibited higher dN/dS than those GSCs_Spermatogonia. Genes biased towards GSCs_Spermatogonia displayed Interestingly, demonstrated lower contrast to spermatids, revealing complexity evolutionary adaptations Furthermore, mitochondria associated events, including oxidative phosphorylation, TCA cycle pyruvate metabolism, were significantly enriched subpopulations. Specifically, function was impaired process ageing, concurrently emphasising role several key nuclear genome‐encoded regulatory genes, such as Hsp60B, fzo, Tim17b1 mRpL12. data offer insights into homeostasis regulated process.

Language: Английский

Tissue-Specific Diversity of Nuclear-Encoded Mitochondrial Genes Related to Lipid and Carbohydrate Metabolism in Buffalo DOI

E. M. Sadeesh,

Madhuri S. Lahamge, Sweta Kumari

et al.

Molecular Biotechnology, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 4, 2025

Language: Английский

Citations

1
Differential expression of nuclear-derived mitochondrial succinate dehydrogenase genes in metabolically active buffalo tissues DOI

E. M. Sadeesh,

Anuj Malik,

Madhuri S. Lahamge

et al.

Molecular Biology Reports, Journal Year: 2024, Volume and Issue: 51(1)

Published: Oct. 19, 2024

Language: Английский

Citations

5
Neuroprotective Effects of Berberine Chloride Against the Aluminium Chloride-Induced Alzheimer’s Disease in Zebra Fish Larvae DOI
Deenathayalan Uvarajan,

Roselin Gnanarajan,

Panimalar Abirami Karuppusamy

et al.

Molecular Biotechnology, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 27, 2025

Language: Английский

Citations

0
Unveiling the tissue-specific landscape of nuclear-encoded mitochondrial genes involved in amino acid metabolism in buffalo DOI Creative Commons

E. M. Sadeesh,

Madhuri S. Lahamge

Amino Acids, Journal Year: 2025, Volume and Issue: 57(1)

Published: Feb. 28, 2025

Mitochondria play a pivotal role in energy production, metabolism, and cellular signaling, serving as key regulators of functions, including differentiation tissue-specific adaptation. The interplay between mitochondria the nucleus is crucial for coordinating these processes, particularly through supply metabolites epigenetic modifications that facilitate nuclear-mitochondrial interactions. To investigate mitochondrial adaptations at molecular level, we conducted RNA sequencing data analyses kidney, heart, brain, ovary tissues female buffaloes, focusing on variations gene expression related to amino acid metabolism. Our analysis identified 82 nuclear-encoded transcripts involved with significant differential patterns across all tissues. Notably, kidney—tissues higher demands—exhibited elevated levels compared ovary. kidney displayed unique patterns, characterized by up-regulation genes glyoxylate metabolism catabolism. In contrast, comparative heart versus brain revealed shared associated fatty oxidation. Gene ontology KEGG pathway confirmed enrichment pathways degradation These findings highlight regulation linked reflecting distinct metabolic requirements different buffalo. Importantly, our results underscore relevance not only livestock health but also understanding disorders humans. By elucidating mechanisms function their variations, this study provides insights could inform breeding strategies enhanced productivity contribute therapeutic approaches human diseases. Thus, illustrate how are specialized manner optimize utilization maintain homeostasis, implications both animal welfare health.

Language: Английский

Citations

0
Effect of glutathione addition on vitrification of ovine oocytes DOI
Jingyu Ren, Gang Liu,

Huan Cui

et al.

Theriogenology, Journal Year: 2025, Volume and Issue: unknown, P. 117412 - 117412

Published: March 1, 2025

Language: Английский

Citations

0
Deciphering tissue-specific expression profiles of mitochondrial genome-encoded tRNAs and rRNAs through transcriptomic profiling in buffalo DOI

E. M. Sadeesh,

Anuj Malik

Molecular Biology Reports, Journal Year: 2024, Volume and Issue: 51(1)

Published: July 31, 2024

Language: Английский

Citations

2
Single‐Cell Transcriptomics Uncovers Core Signature for Regulating Mitochondrial Homeostasis During Testicular Ageing DOI Creative Commons
Weijie Xu,

Qiuru Huang,

Yujuan Qi

et al.

Cell Proliferation, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 27, 2024

ABSTRACT Testicular ageing is accompanied by a series of morphological changes, while the features mitochondrial dysfunction remain largely unknown. Herein, we observed range age‐related modifications in testicular morphology and spermatogenic cells, conducted single‐cell RNA sequencing on young old testes Drosophila . Pseudotime trajectory revealed significant changes germline subpopulations during ageing. Our examination unveiled that genes showing bias spermatids exhibited higher dN/dS than those GSCs_Spermatogonia. Genes biased towards GSCs_Spermatogonia displayed Interestingly, demonstrated lower contrast to spermatids, revealing complexity evolutionary adaptations Furthermore, mitochondria associated events, including oxidative phosphorylation, TCA cycle pyruvate metabolism, were significantly enriched subpopulations. Specifically, function was impaired process ageing, concurrently emphasising role several key nuclear genome‐encoded regulatory genes, such as Hsp60B, fzo, Tim17b1 mRpL12. data offer insights into homeostasis regulated process.

Language: Английский

Citations

1