Published: Jan. 1, 2024
Language: Английский
Biomolecules, Journal Year: 2024, Volume and Issue: 14(3), P. 307 - 307
Published: March 5, 2024
Purpose: to determine the metabolomics profiles in plasma samples of primary open-angle glaucoma (POAG) patients. Methods: The from 20 POAG patients under intraocular pressure (IOP)-lowering medication treatment and control subjects were subjected untargeted analysis, among which 10 further oxylipin-targeted analysis by liquid chromatography–mass spectrometry analysis. prediction accuracy differentially abundant metabolites was assessed receiver operating characteristic curves. Pathway correlation on clinical biochemical parameters also conducted. Results: Untargeted profiling identified 33 patients, metabolism linoleic acid, α-linolenic phenylalanine, tricarboxylic acid cycle enriched. indicated that associated with central corneal thickness, peripapillary retinal nerve fiber layer visual field defects, lymphocytes. 15-keto-Prostaglandin F2 alpha, 13,14-Dihydro-15-keto-prostaglandin D2, 11-Dehydro-thromboxane B2, 8,9-Epoxyeicosatrienoic arachidonic be significantly decreased enriched (AA) pathway. Conclusions: This study revealed pathway are abundant, suggesting high IOP may not only detrimental factor for optic cell damage this group Lipid instability-mediated alterations oxylipins AA pathways important POAG, oxidative stress immune-related inflammation could valuable directions future therapeutic strategies.
Language: Английский
Citations
4
Biochemistry and Biophysics Reports, Journal Year: 2025, Volume and Issue: 41, P. 101943 - 101943
Published: Feb. 18, 2025
Language: Английский
Citations
0
Phytomedicine, Journal Year: 2025, Volume and Issue: 143, P. 156806 - 156806
Published: May 6, 2025
Language: Английский
Citations
0
International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(6), P. 3260 - 3260
Published: March 13, 2024
Fiber diameter is an important characteristic that determines the quality and economic value of rabbit wool. This study aimed to investigate genetic determinants wool fiber through integration analysis using transcriptomic proteomic datasets from hair follicles coarse fine Angora rabbits. Using a 4D label-free technique, we identified 423 differentially expressed proteins (DEPs) in Eighteen DEPs were examined parallel reaction monitoring, which verified reliability our data. Functional enrichment revealed set biological processes signaling pathways related growth strongly enriched by with fold changes greater than two, such as keratinocyte differentiation, skin development, epidermal epithelial cell epidermis epithelium keratinization, estrogen pathway. Association protein–protein interaction network further showed keratin (KRT) family members, including KRT77, KRT82, KRT72, KRT32, KRT10, well CASP14 CDSN, might be key factors contributing differences diameter. Our results promoted understanding molecular mechanisms underlying variation among
Language: Английский
Citations
3
Food Bioscience, Journal Year: 2024, Volume and Issue: 59, P. 103804 - 103804
Published: March 20, 2024
Language: Английский
Citations
2
Science Progress, Journal Year: 2024, Volume and Issue: 107(1)
Published: Jan. 1, 2024
The underlying mechanisms for the beneficial effects exerted by bone marrow-mesenchymal stem cells (BM-MSCs) in treating repetitive traumatic brain injury (rTBI)-induced long-term sensorimotor/cognitive impairments are not fully elucidated. Herein, we aimed to explore whether BM-MSCs therapy protects against rTBI-induced neurobehavioral disorders rats via normalizing white matter integrity and gray microglial response. Rats were subjected repeated mild lateral fluid percussion on day 0 3. On fourth post-surgery, MSCs groups received (4 × 10 6 cells/ml/kg, intravenously) assessed radial maze, Y passive avoidance tests, modified neurological severity scores. Hematoxylin & eosin, Luxol fast blue stainings used examine histopathology thickness. At same time, immunofluorescence staining was investigate numbers of tumor necrosis factor-alpha (TNF-α)-containing microglia matter. Three nine months after neurotrauma, displayed sensorimotor cognitive impairments, reduced thickness matter, over-accumulation TNF-α-containing cellular damage Therapy with significantly attenuated all their complications. Mesenchymal cell might accelerate recovery rTBI myelin
Language: Английский
Citations
2
Molecular Pharmaceutics, Journal Year: 2024, Volume and Issue: 21(3), P. 1334 - 1341
Published: Feb. 19, 2024
Parkinson's disease (PD) is a severe pathology that caused by progressive degeneration of dopaminergic neurons in substantia nigra pars compacta as well other areas the brain. These neurodegeneration processes are linked to abrupt aggregation α-synuclein (α-syn), small protein abundant at presynaptic nerve termini, where it regulates cell vesicle trafficking. Due direct interactions α-syn with membranes, substantial amount work was done over past decade understand role lipids aggregation. However, phosphatidic acid (PA), negatively charged phospholipid polar head, remains unclear. In this study, we examined effect PA large unilamellar vesicles (LUVs) on We found LUVs 16:0, 18:0, and 18:1 FAs drastically reduced toxicity fibrils if were present 1:1 molar ratio protein. Our results also showed presence these vehicles changed rate altered morphology secondary structure fibrils. indicate can be used potential therapeutic strategy reduce formed upon PD.
Language: Английский
Citations
1
Metabolites, Journal Year: 2024, Volume and Issue: 14(1), P. 75 - 75
Published: Jan. 22, 2024
Nutrients involved in the metabolism of inorganic arsenic (iAs) may play a crucial role mitigating adverse health effects associated with such exposure. Consequently, objective this study was to analyze association between intake levels nutrients iAs and alterations metabolic profile during The cohort comprised environmentally exposed women: WL (lower total urinary (As), n = 73) WH (higher As, 73). analysis included untargeted metabolomics (conducted via liquid chromatography–mass spectrometry) assessment nutrient metabolism, specifically methionine, vitamins B2, B6, B12, folate, zinc (based on 3-day dietary records food beverages). In group, all analyzed exhibited negative correlation 5 metabolites (argininosuccinic acid, 5-hydroxy-L-tryptophan, 11-trans-LTE4, mevalonic aminoadipic acid), while it correlated 10 (5-hydroxy-L-tryptophan, dihyroxy-1H-indole glucuronide I, isovalerylglucuronide, 18-oxocortisol, 3-hydroxydecanedioic S-3-oxodecanoyl cysteamine, L-arginine, p-cresol glucuronide, thromboxane B2). Furthermore, demonstrated positive 3 group (inosine, deoxyuridine, glutamine) N-acetyl-L-aspartic tetrahydrodeoxycorticosterone). Altering could be pivotal factor reducing impact exposure human body. This underscores significance maintaining adequate intake, particularly populations arsenic.
Language: Английский
Citations
0
ACS Chemical Neuroscience, Journal Year: 2024, Volume and Issue: 15(3), P. 527 - 538
Published: Jan. 25, 2024
Hair emerged as a biospecimen for long-term investigation of endogenous metabolic perturbations, reflecting the chemical composition circulating in blood over past months. Despite its potential, use human hair metabolomics Alzheimer's disease (AD) research remains limited. Here, we performed both untargeted and targeted metabolomic approaches to profile key pathways 5xFAD mice, widely used AD mouse model. Furthermore, applied discovered metabolites subjects. samples were collected from 6-month-old stage marked by widespread accumulation amyloid plaques brain, followed sample preparation high-resolution mass spectrometry analysis. Forty-five discriminatory mice compared wild-type control mice. Enrichment analysis revealed three pathways: arachidonic acid metabolism, sphingolipid alanine, aspartate, glutamate metabolism. Among these pathways, six demonstrated significant differences 2-month-old prior onset plaque deposition. These findings suggest their potential involvement early stages pathogenesis. When evaluating 45 distinguishing patients with nondemented controls, combination l-valine significantly differentiated two groups, achieving 0.88 area under curve. Taken together, highlight identifying disease-specific alterations developing biomarkers improving detection monitoring.
Language: Английский
Citations
0
Published: Jan. 1, 2024
Language: Английский
Citations
0