Connexin 43 contributes to perioperative neurocognitive disorder by attenuating perineuronal net of hippocampus in aged mice DOI Creative Commons
Qian Zhang, Yuxin Zhang, Peilin Cong

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 16, 2024

Abstract Background Perioperative neurocognitive disorder (PND) is a prevalent form of cognitive impairment in elderly patients following anesthesia and surgery. The underlying mechanisms PND are closely related to perineuronal nets (PNNs). PNNs, which complexes extracellular matrix primarily surrounding neurons the hippocampus, play critical role function. Connexin 43 (Cx43) contributes function by modulating components PNNs. This study was designed investigate specific regulatory Cx43 on PNNs its pivotal development PND. Methods Eighteen-month-old wild-type Gja1fl/fl C57BL/6 mice were subjected abdominal surgery under 1.4% isoflurane anesthesia. Cognitive functions, particularly learning memory, evaluated via Y-maze test, Barnes maze (BM) contextual fear conditioning test (CFT). mRNA protein expression levels assessed using quantitative reverse transcription polymerase chain reaction (qRT-PCR), fluorescent situ hybridization (FISH), western blotting flow cytometry. quantity measured Wisteria floribunda agglutinin (WFA) Aggrecan staining. Results Aged exhibited deficits hippocampus-dependent accompanied increased expression. Conditional knockout (cKO) astrocytes alleviated promoted number dendritic spines hippocampus targeting Dmp1. Knockdown Dmp1 attenuated beneficial effects cKO induced Conclusion Our findings indicate that induce an increase expression, inhibits formation suppressing transcription, leading aged mice. These results offer new mechanistic insights into pathogenesis identify potential targets for therapeutic intervention.

Language: Английский

Connexin 43 contributes to perioperative neurocognitive disorder by attenuating perineuronal net of hippocampus in aged mice DOI Creative Commons
Qian Zhang, Yuxin Zhang, Peilin Cong

et al.

Cellular and Molecular Life Sciences, Journal Year: 2025, Volume and Issue: 82(1)

Published: Jan. 6, 2025

Perioperative neurocognitive disorder (PND) is a prevalent form of cognitive impairment in elderly patients following anesthesia and surgery. The underlying mechanisms PND are closely related to perineuronal nets (PNNs). PNNs, which complexes extracellular matrix primarily surrounding neurons the hippocampus, play critical role function. Connexin 43 (Cx43) contributes function by modulating components PNNs. This study was designed investigate specific regulatory Cx43 on PNNs its pivotal development PND. Eighteen-month-old wild-type Gja1fl/fl C57BL/6 mice were subjected abdominal surgery under 1.4% isoflurane anesthesia. Cognitive functions, particularly learning memory, evaluated via Y-maze test, Barnes maze (BM) contextual fear conditioning test (CFT). mRNA protein expression levels assessed using quantitative reverse transcription polymerase chain reaction (qRT-PCR), fluorescent situ hybridization (FISH), western blotting flow cytometry. quantity measured Wisteria floribunda agglutinin (WFA) Aggrecan staining. Aged exhibited deficits hippocampus-dependent accompanied increased expression. Conditional knockout (cKO) astrocytes alleviated promoted number dendritic spines hippocampus targeting Dmp1. Knockdown Dmp1 attenuated beneficial effects cKO induced Our findings indicate that induce an increase expression, inhibits formation suppressing transcription, leading aged mice. These results offer new mechanistic insights into pathogenesis identify potential targets for therapeutic intervention.

Language: Английский

Citations

0
Improvements in Exercise for Alzheimer’s Disease: Highlighting FGF21-Induced Cerebrovascular Protection DOI
Juan Wang, Xiangyuan Meng,

Yang Jialun

et al.

Neurochemical Research, Journal Year: 2025, Volume and Issue: 50(2)

Published: Feb. 4, 2025

Language: Английский

Citations

0
Vagal activity mediates the relationship between active housework and delayed neurocognitive recovery in elderly patients: a prospective nested case-control study DOI Creative Commons

Junfang Niu,

Xiang Liu,

Xupeng Wang

et al.

BMC Anesthesiology, Journal Year: 2025, Volume and Issue: 25(1)

Published: Feb. 21, 2025

Regular physical activity has been shown to ameliorate cognitive decline associated with aging, and improve autonomic nervous function. However, the effect of active housework, a form light moderate activity, on postoperative function in elderly remain unknown. The aim this study is investigate whether housework exerts protective against delayed neurocognitive recovery (dNCR), explore role vagal relationship. This prospective nested case-control that enrolled 152 patients scheduled for elective knee replacement surgery. neuropsychological assessments were conducted evaluate dNCR both preoperatively one week postoperatively. Physical levels quantified based Activity Scale Elderly categorized into sedentary behavior groups using hierarchical clustering. High-frequency spectral analysis heart rate variability was used assess activity. Multivariable logistic regression employed examine association between dNCR. Additionally, mediation performed possible mediating occurred 33/141 (23.4%) patients. Active 84.7% lower odds developing [OR = 0.153, 95% CI (0.042 0.550), p 0.004]. There significant interaction 0.003, (0.001 0.052), < 0.001]. Vagal mediated dNCR, accounting 31.92% effect. decreased incidence serving as factor. provides valuable insights predicting preventing perioperative disorders Trial registration number: ChiCTR2300070834, date registration: April 24, 2023.

Language: Английский

Citations

0
Microglia phagocytosis of PNNs mediates PV-positive interneuron dysfunction and associated gamma oscillations in neuroinflammation-induced cognitive impairment in mice DOI
Kai Liu,

Yu-Zhu Gao,

Xinmiao Wu

et al.

Neuropharmacology, Journal Year: 2024, Volume and Issue: 262, P. 110205 - 110205

Published: Nov. 2, 2024

Language: Английский

Citations

2
Postoperative cognitive dysfunction in elderly postcardiac surgery patients: progress in rehabilitation application research DOI Creative Commons
Zhenrong Zhang,

Yumei Li,

Xiaoqing Wu

et al.

Frontiers in Rehabilitation Sciences, Journal Year: 2024, Volume and Issue: 5

Published: Dec. 12, 2024

Postoperative cognitive dysfunction (POCD) is a prevalent complication of the central nervous system in elderly patients following cardiac surgery. This review aims to provide an overview etiology, risk factors, diagnostic assessment, and rehabilitation strategies for occurring after The pathogenesis POCD surgery includes cerebral microembolism, neuroinflammation, cryptogenic strokes. Risk factors are associated with advanced age, diminished preoperative status, anesthesia. Cognitive function screening tools used pre- postoperative assessments can detect changes patients’ levels timely manner. provision appropriate methods, including training, exercise transcranial direct current stimulation, perioperative acupuncture, crucial, emerging technologies such as virtual reality playing increasingly significant role. In conclusion, common patients, age reduced being primary factors. A comprehensive strategy more effectively address patients.

Language: Английский

Citations

0
Connexin 43 contributes to perioperative neurocognitive disorder by attenuating perineuronal net of hippocampus in aged mice DOI Creative Commons
Qian Zhang, Yuxin Zhang, Peilin Cong

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 16, 2024

Abstract Background Perioperative neurocognitive disorder (PND) is a prevalent form of cognitive impairment in elderly patients following anesthesia and surgery. The underlying mechanisms PND are closely related to perineuronal nets (PNNs). PNNs, which complexes extracellular matrix primarily surrounding neurons the hippocampus, play critical role function. Connexin 43 (Cx43) contributes function by modulating components PNNs. This study was designed investigate specific regulatory Cx43 on PNNs its pivotal development PND. Methods Eighteen-month-old wild-type Gja1fl/fl C57BL/6 mice were subjected abdominal surgery under 1.4% isoflurane anesthesia. Cognitive functions, particularly learning memory, evaluated via Y-maze test, Barnes maze (BM) contextual fear conditioning test (CFT). mRNA protein expression levels assessed using quantitative reverse transcription polymerase chain reaction (qRT-PCR), fluorescent situ hybridization (FISH), western blotting flow cytometry. quantity measured Wisteria floribunda agglutinin (WFA) Aggrecan staining. Results Aged exhibited deficits hippocampus-dependent accompanied increased expression. Conditional knockout (cKO) astrocytes alleviated promoted number dendritic spines hippocampus targeting Dmp1. Knockdown Dmp1 attenuated beneficial effects cKO induced Conclusion Our findings indicate that induce an increase expression, inhibits formation suppressing transcription, leading aged mice. These results offer new mechanistic insights into pathogenesis identify potential targets for therapeutic intervention.

Language: Английский

Citations

0