Impairment of Skeletal Muscle Contraction by Inhibitors of GABA Transporters DOI Open Access
Nikita S. Fedorov, Guzel Sibgatullina, Artem I. Malomouzh

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(23), P. 12510 - 12510

Published: Nov. 21, 2024

γ-Aminobutyric acid (GABA) has a significant impact on the functioning of not only central but also peripheral part nervous system. Recently, various elements GABAergic signaling system have been discovered in area neuromuscular junction mammals. At same time, functional activity membrane-bound GABA transporters (GATs) and their role transmission identified. In present study, performed preparation mouse diaphragm, effect transporter inhibitors (nipecotic β-alanine) force muscle contraction was assessed. It found that presence both compounds bathing solution, contractions caused by stimulation motor nerve dropped 30-50%. However, when stimulated directly, no contractile observed. The depressant β-alanine induced completely abolished

Language: Английский

25-Hydroxycholesterol modulates synaptic vesicle endocytosis at the mouse neuromuscular junction DOI
Eva A. Kuznetsova,

Guzalia F. Zakirjanova,

Andrei N. Tsentsevitsky

et al.

Pflügers Archiv - European Journal of Physiology, Journal Year: 2025, Volume and Issue: 477(3), P. 421 - 439

Published: Jan. 9, 2025

Language: Английский

Citations

2
Mechanism of Purinergic Regulation of Neurotransmission in Mouse Neuromuscular Junction: The Role of Redox Signaling and Lipid Rafts DOI
А. R. Giniatullin,

Kamilla A. Mukhutdinova,

Alexey M. Petrov

et al.

Neurochemical Research, Journal Year: 2024, Volume and Issue: 49(8), P. 2021 - 2037

Published: May 30, 2024

Language: Английский

Citations

6
Functional and Structural Changes in Diaphragm Neuromuscular Junctions in Early Aging DOI Open Access
Andrei N. Tsentsevitsky, Guzel Sibgatullina,

Yulia G. Odoshivkina

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(16), P. 8959 - 8959

Published: Aug. 17, 2024

Age-related impairment of the diaphragm causes respiratory complications. Neuromuscular junction (NMJ) dysfunction can be one triggering events in weaknesses old age. Prominent structural and functional alterations NMJs were described elderly rodents, but NMJ changes middle age remain unclear. Here, we compared muscles from young adult (3 months) middle-aged (12 BALB/c mice. Microelectrode recordings, immunofluorescent staining, electron microscopy, myography, whole-body plethysmography used. We revealed presynaptic (i) postsynaptic (ii) changes. The former included an increase both action potential propagation velocity neurotransmitter release evoked by low-, moderate-, high-frequency activity a decrease immunoexpression synapsin 1 synaptic vesicle clustering. latter consisted currents via nicotinic acetylcholine receptors area their distribution. These correlated with increased contractile responses to moderate- nerve activation. Additionally, found pattern respiration (an peak inspiratory flow tendency elevation tidal volume), which imply conclude that enhancement neuromuscular communication (due mechanism) accompanied improved occurs early aging.

Language: Английский

Citations

4
25-Hydroxycholesterol as a negative regulator of diaphragm muscle contractions via estrogen receptor and Ca2+ -dependent pathway DOI
Eva A. Kuznetsova, Nikita S. Fedorov,

Guzel F. Zakyrjanova

et al.

Histochemistry and Cell Biology, Journal Year: 2025, Volume and Issue: 163(1)

Published: April 3, 2025

Language: Английский

Citations

0
Key genes and processes affected by atorvastatin treatment in mouse diaphragm muscle DOI

Guzel F. Zakyrjanova,

Valeriya A Matigorova,

Eva A. Kuznetsova

et al.

Archives of Toxicology, Journal Year: 2025, Volume and Issue: unknown

Published: April 16, 2025

Language: Английский

Citations

0
Glutamate and Its Derivatives in Regulation of Peripheral Cholinergic Neurotransmission DOI

N. S. Fedorov,

E. S. Nevsky,

A. R. Tokmakova

et al.

Journal of Evolutionary Biochemistry and Physiology, Journal Year: 2025, Volume and Issue: 61(2), P. 369 - 386

Published: March 1, 2025

Language: Английский

Citations

0
INFLUENCE OF β2-ADRENERGIC SELECTIVE AGONIST FORMOTEROL ON THE MOTOR UNIT OF A MOUSE MODEL OF A CONGENITAL MYASTHENIC SYNDROME WITH COMPLETE VAChT DELETION DOI
Leonardo Rossi,

Bárbara I. Mota,

Priscila Aparecida Costa Valadão

et al.

Neuropharmacology, Journal Year: 2024, Volume and Issue: 260, P. 110116 - 110116

Published: Aug. 14, 2024

Language: Английский

Citations

1
GABA Receptors and Kv7 Channels as Targets for GABAergic Regulation of Acetylcholine Release in Frog Neuromuscular Junction DOI
Andrei N. Tsentsevitsky, Guzel Sibgatullina, Alexey M. Petrov

et al.

Neurochemical Research, Journal Year: 2024, Volume and Issue: 50(1)

Published: Nov. 20, 2024

Language: Английский

Citations

1
Lipid remodeling in acrosome exocytosis: unraveling key players in the human sperm DOI Creative Commons
Laila Suhaiman,

Silvia A. Belmonte

Frontiers in Cell and Developmental Biology, Journal Year: 2024, Volume and Issue: 12

Published: Sept. 23, 2024

It has long been thought that exocytosis was driven exclusively by well-studied fusion proteins. Some decades ago, the role of lipids became evident and escalated interest in field. Our laboratory chose a particular cell to face this issue: human sperm. What makes special? Sperm, as terminal cells, are characterized their scarcity organelles complete absence transcriptional translational activities. They specialized for singular membrane occurrence: acrosome. This unique trait them invaluable study isolation. We will discuss lipids’ sperm acrosome from various perspectives, with primary emphasis on our contributions Sperm cells have lipid composition, very rare not observed many types, comprising high content plasmalogens, long-chain, very-long-chain polyunsaturated fatty acids constituents some sphingolipids. review endeavors unravel impact composition proper functioning exocytic pathway how dynamic influences its fertilizing capability. Evidence other laboratories allowed unveiling importance multiple drive exocytosis. highlights cholesterol, diacylglycerol, phospholipids like phosphatidic acid, phosphatidylinositol 4,5-bisphosphate, sphingolipids driving Furthermore, we provide comprehensive overview factors enzymes regulate turnover during course. A more thorough grasp played transferred can insights into certain causes male infertility. may lead enhancements diagnosing infertility techniques assisted reproductive technology (ART).

Language: Английский

Citations

0
Impairment of Skeletal Muscle Contraction by Inhibitors of GABA Transporters DOI Open Access
Nikita S. Fedorov, Guzel Sibgatullina, Artem I. Malomouzh

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(23), P. 12510 - 12510

Published: Nov. 21, 2024

γ-Aminobutyric acid (GABA) has a significant impact on the functioning of not only central but also peripheral part nervous system. Recently, various elements GABAergic signaling system have been discovered in area neuromuscular junction mammals. At same time, functional activity membrane-bound GABA transporters (GATs) and their role transmission identified. In present study, performed preparation mouse diaphragm, effect transporter inhibitors (nipecotic β-alanine) force muscle contraction was assessed. It found that presence both compounds bathing solution, contractions caused by stimulation motor nerve dropped 30-50%. However, when stimulated directly, no contractile observed. The depressant β-alanine induced completely abolished

Language: Английский

Citations

0