Molecular Neurobiology, Journal Year: 2024, Volume and Issue: unknown
Published: Nov. 14, 2024
Language: Английский
Life Sciences, Journal Year: 2025, Volume and Issue: 363, P. 123355 - 123355
Published: Jan. 6, 2025
Language: Английский
Citations
1
Animals, Journal Year: 2025, Volume and Issue: 15(4), P. 581 - 581
Published: Feb. 18, 2025
N6-methyladenosine (m6A) is one of the most abundant modifications in eukaryotic RNA molecules and mediates functional exertion molecules. We characterized circPAPPA validated its potential m6A modification sites secondary hair follicles (SHFs) cashmere goats. Furthermore, we generated integrated regulatory networks along with enrichment analysis signaling pathways. also explored relationship expression first intron methylation PAPPA gene SHFs Host source revealed that derived from complete exon 2 gene, spliced reverse orientation, predominantly localized cytoplasm SHF stem cells The was verified to contain at least four goats, including m6A-450/456, m6A-852, m6A-900, m6A-963. network indicated complex diverse relationships m6A-circPAPPA putative molecules, miRNAs, mRNAs, proteins. Enrichment pathways showed might play multiple roles growth development goats through mediated by target miRNAs likely significantly involved dynamic goat SHFs. Results this study provided novel information elucidate biological fiber.
Language: Английский
Citations
0
Molecular Biology Reports, Journal Year: 2024, Volume and Issue: 52(1)
Published: Nov. 28, 2024
Language: Английский
Citations
2
Molecular and Cellular Biochemistry, Journal Year: 2024, Volume and Issue: unknown
Published: Nov. 4, 2024
Stroke, as a neurological disorder with poor overall prognosis, has long plagued the patients. Current stroke therapy lacks effective treatments. Ferroptosis emerged prominent subject of discourse across various maladies in recent years. As an emerging therapeutic target, notwithstanding its initial identification tumor cells associated brain diseases, it lately been recognized pivotal factor pathological progression stroke. Acyl-CoA synthetase long-chain family member 4 (ACSL4) is potential target and biomarker catalytic unsaturated fatty acids mediating ferroptosis Specifically, upregulation ACSL4 leads to heightened accumulation lipid peroxidation products reactive oxygen species (ROS), thereby exacerbating neuronal cells. present tissues involved multiple pathways ferroptosis. At present, pharmacological mechanisms targeting inhibit have found many drugs, but molecular are still exploratory stage. This paper introduces physiopathological mechanism current status research crosstalk epigenetics, summarizes application modern pharmacology research, discusses value field
Language: Английский
Citations
1
Brain Research Bulletin, Journal Year: 2024, Volume and Issue: 220, P. 111156 - 111156
Published: Nov. 30, 2024
This study explores the molecular mechanism of METTL14 regulating microglial function post ischemic stroke. A murine model was established by tMCAO. The neurological evaluated mNSS. cerebral infarct size and pathological changes were observed TTC H&E staining. M1 M2 microglia in brain tissues detected flow cytometry. BV2 cells subjected to OGD/R establish an vitro model. qRT-PCR Western blot used for detecting METTL14, PAX6, YTHDF2, TREM2, iNOS, Arg1 expressions. m6A level quantitatively analyzed, binding YTHDF2 or PAX6 analyzed RIP. mRNA stability assessed after actinomycin D treatment. ChIP utilized determining enrichment on TREM2 promoter. relationship between verified dual-luciferase reporter assay. highly expressed tMCAO, silence alleviated symptoms tMCAO mice promoted polarization. enhanced modification promote its degradation. bound promoter facilitated transcription expression. In conclusion, METTL14-mediated aggravates stroke promoting polarization via YTHDF2/PAX6/TREM2 axis.
Language: Английский
Citations
1
Molecular Neurobiology, Journal Year: 2024, Volume and Issue: unknown
Published: Nov. 14, 2024
Language: Английский
Citations
0