Fluoride Alters Gene Expression via Histone H3K27 Acetylation in Ameloblast-like LS8 Cells DOI Open Access
Shohei Yamashita, Motoki Okamoto,

Melanie Mendonca

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(17), P. 9600 - 9600

Published: Sept. 4, 2024

Excessive fluoride ingestion during tooth development can cause dental fluorosis. Previously, we reported that activates histone acetyltransferase (HAT) to acetylate p53, promoting toxicity in mouse ameloblast-like LS8 cells. However, the roles of HAT and acetylation status fluoride-mediated gene expression remain unidentified. Here, demonstrate modification causes alterations cells were treated with or without followed by ChIP-Seq analysis H3K27ac. Genes identified differential H3K27ac peaks within ±1 kb from transcription start sites. The levels mRNA genes assessed using rea-time PCR (qPCR). Fluoride increased associated Bax, p21, Mdm2 upregulated their levels. decreased Bad, Bcl2 had suppressed transcription. inhibitors (Anacardic acid MG149) fluoride-induced p21 Mdm2, while deacetylase (HDAC) inhibitor sodium butyrate Bad above treatment alone. To our knowledge, this is first study demonstrates epigenetic regulation via H3 acetylation. Further investigation required elucidate mechanisms enamel development.

Language: Английский

Histone Deacetylation in Alzheimer’s Diseases (AD); Hope or Hype DOI

Nabaa Hisham Ateya,

Sarah F. Al-Taie,

Saade Abdalkareem Jasim

et al.

Cell Biochemistry and Biophysics, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 18, 2025

Language: Английский

Citations

0
Inhibitor of Growth Proteins: Epigenetic Regulators Shaping Neurobiology DOI Creative Commons
Ziyue Xu,

Hongyu Xu,

Jichun Shi

et al.

Biomolecules, Journal Year: 2025, Volume and Issue: 15(2), P. 281 - 281

Published: Feb. 14, 2025

The inhibitor of growth (ING) family proteins is emerging as a pivotal regulator epigenetic modifications within the nervous system. These are involved in various cellular processes, including apoptosis, cell cycle control, and DNA repair, through interactions with chromatin-modifying complexes. Recent studies underscore dual role ING both tumor suppression neuronal differentiation, development, neuroprotection. This review summarizes functions neurobiology, focus on their involvement neural development relevance to neuro-oncological diseases. We explore mechanisms by which influence chromatin state gene expression, highlighting histone acetyltransferases, deacetylases, methyltransferases, modification enzymes, non-coding RNAs. A deeper understanding regulation system may pave way for novel therapeutic strategies targeting neurological disorders.

Language: Английский

Citations

0
Acetylation and deacetylation dynamics in stress response to cancer and infections DOI

Lili Li,

Yanqiong Zeng,

Genhong Cheng

et al.

Seminars in Immunology, Journal Year: 2025, Volume and Issue: 78, P. 101957 - 101957

Published: April 26, 2025

Language: Английский

Citations

0
Acetylation‐Deacetylation‐Driven Natural Product Biosynthesis DOI Creative Commons
Huibin Wang, Ikuro Abe

ChemistryEurope, Journal Year: 2025, Volume and Issue: unknown

Published: April 28, 2025

Abstract Acetylation introduces acetyl groups to substrates, thus altering their chemical reactivity and stability, plays a crucial role in natural product biosynthesis by driving structural diversification functional optimization. Emerging evidence now highlights cryptic acetylations as transient modifications that guide sequential reactions, enabling the efficient assembly of bioactive molecules. Here, we systematically examine multifaceted roles acetylation‐deacetylation dynamics biosynthesis, with particular emphasis on four key mechanistic paradigms. First, explore strategy acetylation followed deacetylation after several biosynthetic steps, which serves protective directing mechanism. Second, investigate acetylation‐mediated rearrangement, where introduction an group triggers rearrangement generate novel molecular architectures. Third, analyze acetylation‐triggered elimination, process facilitates formation double bonds scaffolds. Finally, discuss cycle regulatory mechanism, highlighting its controlling flux intermediate stability. We also challenges identifying characterizing acetylations, while future opportunities harness these for synthetic biology. By elucidating hidden dynamics, this not only deepens our understanding but provides innovative strategies drug discovery.

Language: Английский

Citations

0
Glutathione S-transferase: A keystone in Parkinson's disease pathogenesis and therapy DOI

Pratyush Padhan,

Simran Simran,

Neeraj Kumar

et al.

Molecular and Cellular Neuroscience, Journal Year: 2024, Volume and Issue: unknown, P. 103981 - 103981

Published: Dec. 1, 2024

Language: Английский

Citations

2
Design, synthesis, and biological evaluation of novel AAK1/HDACs dual inhibitors against SARS-CoV-2 entry DOI

Nian‐Dong Mao,

Yueying Xu, Xia Yao

et al.

Bioorganic Chemistry, Journal Year: 2024, Volume and Issue: 153, P. 107973 - 107973

Published: Nov. 19, 2024

Language: Английский

Citations

1
Design, Synthesis, and Biological Evaluation of Novel Aak1/Hdacs Dual Inhibitors Against Sars-Cov-2 Entry DOI
Xiang‐Yang Ye,

Nian‐Dong Mao,

Yueying Xu

et al.

Published: Jan. 1, 2024

Language: Английский

Citations

0
Glutathione S-Transferases: Potential Implications in Parkinson's Disease. DOI Open Access
Sonia Verma,

Pratyush Padhan,

Simran Simran

et al.

Published: Aug. 8, 2024

Parkinson's disease is a progressive neurodegenerative disorder that predominantly affects motor function due to the loss of dopaminergic neurons in substantia nigra. It presents significant challenges, impacting millions worldwide with symptoms such as tremors, rigidity, bradykinesia, and postural instability, leading decreased quality life increased morbidity. The pathogenesis multifaceted, involving complex interactions between genetic susceptibility, environmental factors, aging, oxidative stress playing central role neuronal degeneration. Glutathione S-Transferase enzymes are critical cellular defense mechanism against stress, catalyzing conjugation antioxidant glutathione various toxic compounds, thereby facilitating their detoxification. Recent research underscores importance pathophysiology disease, revealing polymorphisms genes influence risk progression disease. These variations can affect enzymatic activity S-Transferase, modulating an individual's capacity detoxify reactive oxygen species xenobiotics, which implicated neuropathological processes. Moreover, biochemical studies have elucidated not only maintaining redox balance but also signalling pathways, highlighting its neuroprotective potential. From therapeutic perspective, targeting pathways offers promising avenues for development novel treatments aimed at enhancing neuroprotection mitigating progression. This review explores evident hypothesized roles providing comprehensive overview potential target intervention.

Language: Английский

Citations

0
Fluoride Alters Gene Expression via Histone H3K27 Acetylation in Ameloblast-like LS8 Cells DOI Open Access
Shohei Yamashita, Motoki Okamoto,

Melanie Mendonca

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(17), P. 9600 - 9600

Published: Sept. 4, 2024

Excessive fluoride ingestion during tooth development can cause dental fluorosis. Previously, we reported that activates histone acetyltransferase (HAT) to acetylate p53, promoting toxicity in mouse ameloblast-like LS8 cells. However, the roles of HAT and acetylation status fluoride-mediated gene expression remain unidentified. Here, demonstrate modification causes alterations cells were treated with or without followed by ChIP-Seq analysis H3K27ac. Genes identified differential H3K27ac peaks within ±1 kb from transcription start sites. The levels mRNA genes assessed using rea-time PCR (qPCR). Fluoride increased associated Bax, p21, Mdm2 upregulated their levels. decreased Bad, Bcl2 had suppressed transcription. inhibitors (Anacardic acid MG149) fluoride-induced p21 Mdm2, while deacetylase (HDAC) inhibitor sodium butyrate Bad above treatment alone. To our knowledge, this is first study demonstrates epigenetic regulation via H3 acetylation. Further investigation required elucidate mechanisms enamel development.

Language: Английский

Citations

0