Journal of Chromatography B, Journal Year: 2024, Volume and Issue: 1251, P. 124373 - 124373
Published: Dec. 2, 2024
Language: Английский
Journal of Chromatography B, Journal Year: 2024, Volume and Issue: 1251, P. 124373 - 124373
Published: Dec. 2, 2024
Language: Английский
Current Issues in Molecular Biology, Journal Year: 2025, Volume and Issue: 47(1), P. 68 - 68
Published: Jan. 20, 2025
Hair loss is one of the skin conditions that can affect people’s mental health. Plant raw material extracts are great interest due to their safety. In this study, we utilize reverse network pharmacology screen for key targets Wnt/β-catenin signaling pathway and TGFβ/BMP pathway, as well differential lipids, plant materials selection. The aim identify may have anti-hair properties validate these findings through cell experiments. Licorice, salvia miltiorrhiza, mulberry leaf, ephedra curcumae radix were found possess effects. Licorice water extract (LWE), miltiorrhiza (SMWE), leaf (MLWE), (EWE) (CRWE) did not exhibit cytotoxicity on human dermal papilla cells (HDPCs). Through ALP staining, it was expression in HDPCs treated with LWE, SMWE, MLWE, EWE CRWE enhanced. addition, reduced hair growth inhibitory factor TGF-β1 inflammatory IL-6. Additionally, various enhance secretion VEGF, high concentrations exhibiting better efficacy. Furthermore, β-catenin, a enhanced by treatment cultured HDPCs. conclusion, all five showed some potential, providing theoretical support application products.
Language: Английский
Citations
0PubMed, Journal Year: 2025, Volume and Issue: unknown
Published: Jan. 28, 2025
Language: Английский
Citations
0Food Bioscience, Journal Year: 2025, Volume and Issue: unknown, P. 106030 - 106030
Published: March 1, 2025
Language: Английский
Citations
0Journal of Ethnopharmacology, Journal Year: 2025, Volume and Issue: unknown, P. 119865 - 119865
Published: April 1, 2025
Language: Английский
Citations
0Frontiers in Immunology, Journal Year: 2025, Volume and Issue: 16
Published: April 22, 2025
Introduction Thlaspi arvense (TA), commonly known as “Ximi” or “Subaijiang,” is a traditional Chinese medicinal herb used to prevent and treat ulcerative colitis (UC). However, the precise mechanisms underlying its therapeutic effects remain unclear, necessitating further investigation identify potential pharmaceutical applications for UC management. This study aims elucidate efficacy of TA active constituents in treatment. Methods first evaluated varying doses on 3% dextran sulfate sodium (DSS)-induced UC. Gut microbiota alterations mice were analyzed via 16S rRNA sequencing, with correlation analyses reveal relationship between gut cytokines. Then, network pharmacology was utilized identified targets Protein-protein interaction (PPI) networks, Gene Ontology (GO), Kyoto Encyclopedia Genes Genomes (KEGG) enrichment employed explore TA’s mechanisms. Molecular docking dynamics simulations validated interactions compounds UC-related targets. Finally, TNF pathway modulation by component, isovitexin, verified vitro vivo . Results alleviated DSS-induced weight loss dose-dependent manner, reduced disease activity indices, preserved intestinal mucosal barrier integrity. Subsequently, fluorescence situ hybridization (FISH) revealed suppressed microbial translocation tissues. To characterize inflammatory responses, ELISA demonstrated that modulated levels key cytokines (TNF-α, IL-1β, IL-6, IL-10) oxidative stress markers (SOD, MDA), indicating systemic anti-inflammatory effects. Building these findings, sequencing showed regulated alpha/beta diversity inhibited infectious disease-related pathways. Notably, heatmaps highlighted strong association TNF-α Escherichia-Shigella abundance, high-dose significantly reducing this pathogenic bacterial genus. systematically molecular mechanisms, 220 Consistent experimental data, PPI KEGG implicated TNF-α, AKT targets, primarily through signaling modulation. validate predictions, confirmed stable while specifically emphasized isovitexin’s high affinity TNF-α. experiments inhibition TNF-α-mediated NF-κB activation, studies isovitexin directly mitigated TNF-α-induced epithelial damage. Furthermore, potent activation tissues, constituent effectively cell damage, collectively highlighting their complementary Discussion Collectively, (TA) ameliorates synergistic involving modulation, suppression, preservation. By remodeling communities reduce colonization translocation. concurrently inhibits TNF-α/NF-κB-driven inflammation, regulation. attenuates demonstrating multi-scale efficacy. These findings establish multi-target spanning host-microbe intracellular signaling, providing rationale standardizing TA-based formulations advancing precision agent bowel diseases.
Language: Английский
Citations
0Heliyon, Journal Year: 2024, Volume and Issue: 10(19), P. e38629 - e38629
Published: Sept. 30, 2024
Language: Английский
Citations
0Integrative Medicine in Nephrology and Andrology, Journal Year: 2024, Volume and Issue: 11(4)
Published: Nov. 14, 2024
Background: Benign prostatic hyperplasia (BPH) is a common condition affecting the urinary tract. This study aimed at exploring potential of Guiyi Tonglong (GYTL) prescription for treatment benign using network pharmacology and molecular docking technology. Methods: The main active ingredients in GYTL were identified from Traditional Chinese Medicine Systems Pharmacology Integrative Pharmacology-based (TCMIP) databases based on drug similarity ≥ 30% oral bioavailability 0.18. Protein–protein interaction (PPI) networks, gene ontology, Kyoto Encyclopedia Genes Genomes (KEGG) enrichment analyses used to interactions pathway establish ingredient-target-pathway networks. structure ingredient was docked that protein target AutoDock software. Results: A total 86 130 targets screened prescription, PPI constructed identify five most important core targets, namely SRC, TP53, STAT3, AKT1, PIK3CA. Enrichment analysis revealed may play role BPH through multiple signaling pathways, such as PI3K-Akt, EGFR, AGE-RAGE, HIF-1. Molecular indicated between targets. Conclusion: These results provide further information regarding mechanisms treating BPH.
Language: Английский
Citations
0Journal of Chromatography B, Journal Year: 2024, Volume and Issue: 1251, P. 124373 - 124373
Published: Dec. 2, 2024
Language: Английский
Citations
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