Anti-cystitis glandularis action exerted by glycyrrhetinic acid: bioinformatics analysis and molecular validation.

PubMed, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 28, 2025

Language: Английский

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Evaluation of novel angiotensin I-converting enzyme and dipeptidyl peptidase IV inhibitory peptides derived from yak milk based on peptidomics and network pharmacology

Food Bioscience, Journal Year: 2025, Volume and Issue: unknown, P. 106030 - 106030

Published: March 1, 2025

Language: Английский

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Screening of Anti-Hair Loss Plant Raw Materials Based on Reverse Network Pharmacology and Experimental Validation

Current Issues in Molecular Biology, Journal Year: 2025, Volume and Issue: 47(1), P. 68 - 68

Published: Jan. 20, 2025

Hair loss is one of the skin conditions that can affect people’s mental health. Plant raw material extracts are great interest due to their safety. In this study, we utilize reverse network pharmacology screen for key targets Wnt/β-catenin signaling pathway and TGFβ/BMP pathway, as well differential lipids, plant materials selection. The aim identify may have anti-hair properties validate these findings through cell experiments. Licorice, salvia miltiorrhiza, mulberry leaf, ephedra curcumae radix were found possess effects. Licorice water extract (LWE), miltiorrhiza (SMWE), leaf (MLWE), (EWE) (CRWE) did not exhibit cytotoxicity on human dermal papilla cells (HDPCs). Through ALP staining, it was expression in HDPCs treated with LWE, SMWE, MLWE, EWE CRWE enhanced. addition, reduced hair growth inhibitory factor TGF-β1 inflammatory IL-6. Additionally, various enhance secretion VEGF, high concentrations exhibiting better efficacy. Furthermore, β-catenin, a enhanced by treatment cultured HDPCs. conclusion, all five showed some potential, providing theoretical support application products.

Language: Английский

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Integrated transcriptomic and proteomic analysis of hepatotoxic effects of Venenum Bufonis in zebrafish

Journal of Ethnopharmacology, Journal Year: 2025, Volume and Issue: unknown, P. 119865 - 119865

Published: April 1, 2025

Language: Английский

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Thlaspi arvense suppresses gut microbiota related TNF inflammatory pathway to alleviates ulcerative colitis

Frontiers in Immunology, Journal Year: 2025, Volume and Issue: 16

Published: April 22, 2025

Thlaspi arvense (TA), commonly known as "Ximi" or "Subaijiang," is a traditional Chinese medicinal herb used to prevent and treat ulcerative colitis (UC). However, the precise mechanisms underlying its therapeutic effects remain unclear, necessitating further investigation identify potential pharmaceutical applications for UC management. This study aims elucidate efficacy of TA active constituents in treatment. first evaluated varying doses on 3% dextran sulfate sodium (DSS)-induced UC. Gut microbiota alterations mice were analyzed via 16S rRNA sequencing, with correlation analyses reveal relationship between gut cytokines. Then, network pharmacology was utilized identified targets Protein-protein interaction (PPI) networks, Gene Ontology (GO), Kyoto Encyclopedia Genes Genomes (KEGG) enrichment employed explore TA's mechanisms. Molecular docking dynamics simulations validated interactions compounds UC-related targets. Finally, TNF pathway modulation by component, isovitexin, verified vitro vivo. alleviated DSS-induced weight loss dose-dependent manner, reduced disease activity indices, preserved intestinal mucosal barrier integrity. Subsequently, fluorescence situ hybridization (FISH) revealed suppressed microbial translocation tissues. To characterize inflammatory responses, ELISA demonstrated that modulated levels key cytokines (TNF-α, IL-1β, IL-6, IL-10) oxidative stress markers (SOD, MDA), indicating systemic anti-inflammatory effects. Building these findings, sequencing showed regulated alpha/beta diversity inhibited infectious disease-related pathways. Notably, heatmaps highlighted strong association TNF-α Escherichia-Shigella abundance, high-dose significantly reducing this pathogenic bacterial genus. systematically molecular mechanisms, 220 Consistent experimental data, PPI KEGG implicated TNF-α, AKT targets, primarily through signaling modulation. validate predictions, confirmed stable while specifically emphasized isovitexin's high affinity TNF-α. experiments vivo inhibition TNF-α-mediated NF-κB activation, studies isovitexin directly mitigated TNF-α-induced epithelial damage. Furthermore, potent activation tissues, constituent effectively cell damage, collectively highlighting their complementary Collectively, (TA) ameliorates synergistic involving modulation, suppression, preservation. By remodeling communities reduce colonization translocation. concurrently inhibits TNF-α/NF-κB-driven inflammation, regulation. attenuates demonstrating multi-scale efficacy. These findings establish multi-target spanning host-microbe intracellular signaling, providing rationale standardizing TA-based formulations advancing precision agent bowel diseases.

Language: Английский

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A Direct Relationship Between ‘Blood Stasis’ and Fibrinaloid Microclots in Chronic, Inflammatory, and Vascular Diseases, and Some Traditional Natural Products Approaches to Treatment

Pharmaceuticals, Journal Year: 2025, Volume and Issue: 18(5), P. 712 - 712

Published: May 12, 2025

'Blood stasis' (syndrome) (BSS) is a fundamental concept in Traditional Chinese Medicine (TCM), where it known as Xue Yu (). Similar concepts exist Korean ('Eohyul') and Japanese Kampo medicine (Oketsu). Blood stasis considered to underpin large variety of inflammatory diseases, though an exact equivalent Western systems yet be described. Some time ago we discovered that blood can clot into anomalous amyloid form, creating what have referred fibrinaloid microclots. These microclots occur great many chronic, diseases are comparatively resistant fibrinolysis, thus the ability block microcapillaries hence lower oxygen transfer tissues, with multiple pathological consequences. We here develop idea precisely relate to, largely mechanistically responsible for, traditional (a term also used by Virchow). First, associated all stasis. Secondly, blocking red cell transport, provide simple mechanistic explanation for physical slowing down ('stasis') flow. Thirdly, herbal formulae proposed treat these especially Fu Zhu its derivatives, anticoagulatory anti-inflammatory, consistent they actually helping levels microclots, plausibly part catalysis polymerization fibrinogen form. rehearse some actions constituent herbs specific bioactive molecules contain. Consequently, such formulations (and their components), which little science medicine, would seem offer opportunity novel, safe, useful treatments chronic display including Myalgic Encephalopathy/Chronic Fatigue Syndrome, long COVID, even ischemic stroke.

Language: Английский

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Integrating network pharmacology and experimental verification to study the mechanism of Polygonum hydropiper total flavonoids against stress-induced gastric mucosal damage

Heliyon, Journal Year: 2024, Volume and Issue: 10(19), P. e38629 - e38629

Published: Sept. 30, 2024

Language: Английский

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Network Pharmacology Prediction and Molecular Docking-based Strategy to Explore the Potential Mechanism of Guiyi Tonglong Prescription in the Treatment of Benign Prostatic Hyperplasia

Integrative Medicine in Nephrology and Andrology, Journal Year: 2024, Volume and Issue: 11(4)

Published: Nov. 14, 2024

Background: Benign prostatic hyperplasia (BPH) is a common condition affecting the urinary tract. This study aimed at exploring potential of Guiyi Tonglong (GYTL) prescription for treatment benign using network pharmacology and molecular docking technology. Methods: The main active ingredients in GYTL were identified from Traditional Chinese Medicine Systems Pharmacology Integrative Pharmacology-based (TCMIP) databases based on drug similarity ≥ 30% oral bioavailability 0.18. Protein–protein interaction (PPI) networks, gene ontology, Kyoto Encyclopedia Genes Genomes (KEGG) enrichment analyses used to interactions pathway establish ingredient-target-pathway networks. structure ingredient was docked that protein target AutoDock software. Results: A total 86 130 targets screened prescription, PPI constructed identify five most important core targets, namely SRC, TP53, STAT3, AKT1, PIK3CA. Enrichment analysis revealed may play role BPH through multiple signaling pathways, such as PI3K-Akt, EGFR, AGE-RAGE, HIF-1. Molecular indicated between targets. Conclusion: These results provide further information regarding mechanisms treating BPH.

Language: Английский

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Study on the protective mechanism of Xuemaitong Capsule against acute myocardial ischemia rat based on network pharmacology and metabolomics

Journal of Chromatography B, Journal Year: 2024, Volume and Issue: 1251, P. 124373 - 124373

Published: Dec. 2, 2024

Language: Английский

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