In-vitro and in-silico evaluation of antihyperglycemic, antioxidant, anti-inflammatory activities of selected (2Z)-2-((E)(2'hydroxy)-4(benzylideneamino)phenyl)-3-(1-methyl-1H-imidazol-2-yl)acrylonitrile derivatives

Journal of the Indian Chemical Society, Journal Year: 2025, Volume and Issue: unknown, P. 101592 - 101592

Published: Jan. 1, 2025

Language: Английский

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Stress‐Induced Response and Adaptation Mechanisms in Bacillus licheniformis PSKA1 Exposed With Abiotic and Antibiotic Stresses

Journal of Basic Microbiology, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 2, 2025

ABSTRACT Soil ecosystems consist of diverse microbial communities with great potential for ecological and biotechnological applications. These encounter various abiotic stresses, which expedite the activation transient overexpression heat shock proteins (HSPs). In present study, a soil bacterium was isolated identified as Bacillus licheniformis strain PSK.A1, its growth parameters were optimized before exposing it to heat, salt, pH, antibiotic stress conditions. Comparative protein expression analyzed using SDS‐PAGE, stabilization via aggregation assays, survival through single spot dilution colony‐counting methods under The pre‐treatment short dosage showed endured overall tolerance lethal conditions, evidenced by moderately enhanced total soluble intracellular content, better stabilization, comparatively over‐expressed HSPs, relatively cell survival. findings highlighted that cells grown optimal conditions more susceptible environments than stressed cells, their linked 20 distinct HSPs 17–91 kD. insights offer developing strategies enhance resilience applications including bacterial bioprocessing, bio‐remediation, infectious disease management.

Language: Английский

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Microbial protein sources: A comprehensive review on the potential usage of fungi and cyanobacteria in sustainable food systems

Food and Humanity, Journal Year: 2024, Volume and Issue: 3, P. 100366 - 100366

Published: July 27, 2024

Language: Английский

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A comprehensive review on latent role of stress proteins in antibiotic resistance

The Microbe, Journal Year: 2024, Volume and Issue: 4, P. 100151 - 100151

Published: Aug. 24, 2024

Language: Английский

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New mechanistic insights on transferrin synthesis inhibition and release of bound iron mediated by lead loaded ultrafine carbon black

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: 306, P. 141780 - 141780

Published: March 5, 2025

Language: Английский

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Theoretical Study of Sphingomyelinases from Entamoeba histolytica and Trichomonas vaginalis Sheds Light on the Evolution of Enzymes Needed for Survival and Colonization

Pathogens, Journal Year: 2025, Volume and Issue: 14(1), P. 32 - 32

Published: Jan. 5, 2025

The path to survival for pathogenic organisms is not straightforward. Pathogens require a set of enzymes tissue damage generation and obtain nourishment, as well toolbox full alternatives bypass host defense mechanisms. Our group has shown that the parasitic protist Entamoeba histolytica encodes 14 sphingomyelinases (SMases); one them (acid sphingomyelinase 6, aSMase6) involved in repairing membrane exhibits hemolytic activity. enzymatic characterization aSMase6 been be activated by magnesium ions but zinc, human aSMase, strongly inhibited cobalt. However, no structural data are available enzyme. In this work, bioinformatic analyses showed aSMases diverse enzymes, evolutionarily related hemolysins derived from bacteria, similar overall structure parasitic, free-living protists mammalian enzymes. AlphaFold3 models predicted occupancy cobalt active site Cavity blind docking substrate pushed outward when bound instead ions. Additionally, E. loop absent rest aSMases, suggesting it may activity, demonstrated experimentally using recombinant proteins aSMase4 aSMase6. Trichomonas vaginalis show putative transmembrane domain seem functionally different histolytica. This work provides insight into future biochemical can mechanistic features sphingomyelinases, ultimately rendering these potential therapeutic targets.

Language: Английский

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Exploring of Antibacterial Compounds from Mangrove Endophytic Fungus Aspergillus Sydowii 22plp1-F1 Through Osmac and Computational Approach to Combat Multidrug-Resistant Bacteria

Published: Jan. 1, 2025

Language: Английский

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In Silico Identification of Potential Clovibactin-like Antibiotics Binding to Unique Cell Wall Precursors in Diverse Gram-Positive Bacterial Strains

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(4), P. 1724 - 1724

Published: Feb. 18, 2025

The rise in multidrug-resistant bacteria highlights the critical need for novel antibiotics. This study explores clovibactin-like compounds as potential therapeutic agents targeting lipid II, a crucial component bacterial cell wall synthesis, using silico techniques. A total of 2624 clovibactin analogs were sourced from PubChem database and screened ProTox 3.0 software based on their ADME-Tox properties, prioritizing candidates with favorable pharmacokinetic profiles minimal toxicity. Molecular docking protocols then employed to assess binding interactions selected II. Our analysis identified Compound 22 particularly promising candidate, exhibiting strong affinity, stable complex formation, high selectivity target. Binding energy analysis, conducted via molecular dynamics simulations, revealed highly negative value -25.50 kcal/mol 22, surpassing that underscoring its efficacy. In addition, was prioritized due exceptional affinity II suggesting lower likelihood adverse effects. These characteristics position candidate further pharmacological development. While our computational results are encouraging, experimental validation is essential confirm efficacy safety these compounds. not only advances understanding but also contributes ongoing efforts combat antimicrobial resistance through innovative antibiotic

Language: Английский

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Anticancer effects of silymarin-loaded ACA-HA/sulfonamide IR-MOF nanoplatform in CT-26 xenograft model

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: unknown, P. 142651 - 142651

Published: March 1, 2025

Language: Английский

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Biochemical and Computational Insights into the Therapeutic Potencies of Quinoline Appended Imidazole Compounds

ChemistrySelect, Journal Year: 2025, Volume and Issue: 10(14)

Published: April 1, 2025

Abstract Diabetes is a complex metabolic disorder characterized by oxidative stress and chronic inflammation, necessitating the development of multifunctional therapeutic agents. This study evaluates in vitro silico antihyperglycemic, antioxidant, anti‐inflammatory activities four 3‐(4,5‐diaryl‐1 H ‐imidazol‐2‐yl)quinoline‐2‐amine derivatives (a–d) . Among them, compound (a) exhibited strong antihyperglycemic activity, with significant α‐amylase inhibition (IC 50 = 132.55 ± 4.12 µg/mL) enhanced glucose uptake yeast cells 126.32 3.48 µg/mL). Compound (d) showed superior antioxidant 42–44 properties. Molecular docking against PPAR‐γ confirmed binding interactions for all compounds, showing collectively better affinity (−10 kcal/mol −11.1 kcal/mol). Further, molecular dynamics normal mode analysis validated its stability functional potential. The ADMET predictions suggested favorable pharmacokinetics, especially compounds (b) findings suggest that has potential as lead molecule treatment moderate also shows promise, albeit slightly lower bioactivity. Despite these promising findings, none tested outperformed efficacy standard drugs any assays, indicating need further structural optimization to enhance their

Language: Английский

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