3D cell culture models: how to obtain and characterize the main models DOI Creative Commons

M.M. Abdurakhmanova,

Anastasiia Leonteva,

N. S. Vasilieva

et al.

Vavilov Journal of Genetics and Breeding, Journal Year: 2025, Volume and Issue: 29(2), P. 175 - 188

Published: April 10, 2025

For many years, the gold standard in study of malignant tumors has been vitro culture tumor cells, vivo xenografts or genetically modified animal models. Meanwhile, three-dimensional cell models (3D cultures) have added to arsenal modern biomedical research. 3D cultures reproduce tissue-specific features tissue topology. This makes them relevant terms differentiation, metabolism and development drug resistance. Such are already being used by research groups for both basic translational research, may substantially reduce number studies, example field oncological In current literature, classified according technique their formation (with without a scaffold), cultivation conditions (static dynamic), as well cellular organization function. organization, divided into “spheroid models”, “organoids”, “organs-ona-chip” “microtissues”. Each these its own unique features, which should be taken account when using particular model an experiment. The simplest spheroid floating spherical aggregates. An organoid is more complex model, self-organizing structure formed from stem cells (SCs) capable self-renewal differentiation within model. Organ-on-a-chip chips microfluidic systems that simulate dynamic physical biological processes found organs tissues . By combining different types single structure, spheroids organoids can act basis microtissue – hybrid imitating specific phenotype containing tissuespecific extracellular matrix (ECM) components. review presents brief history culture. It describes main characteristics perspectives use “organ-on-a-chip” “microtissues” immune oncology solid tumors.

Language: Английский

3D cell culture models: how to obtain and characterize the main models DOI Creative Commons

M.M. Abdurakhmanova,

Anastasiia Leonteva,

N. S. Vasilieva

et al.

Vavilov Journal of Genetics and Breeding, Journal Year: 2025, Volume and Issue: 29(2), P. 175 - 188

Published: April 10, 2025

For many years, the gold standard in study of malignant tumors has been vitro culture tumor cells, vivo xenografts or genetically modified animal models. Meanwhile, three-dimensional cell models (3D cultures) have added to arsenal modern biomedical research. 3D cultures reproduce tissue-specific features tissue topology. This makes them relevant terms differentiation, metabolism and development drug resistance. Such are already being used by research groups for both basic translational research, may substantially reduce number studies, example field oncological In current literature, classified according technique their formation (with without a scaffold), cultivation conditions (static dynamic), as well cellular organization function. organization, divided into “spheroid models”, “organoids”, “organs-ona-chip” “microtissues”. Each these its own unique features, which should be taken account when using particular model an experiment. The simplest spheroid floating spherical aggregates. An organoid is more complex model, self-organizing structure formed from stem cells (SCs) capable self-renewal differentiation within model. Organ-on-a-chip chips microfluidic systems that simulate dynamic physical biological processes found organs tissues . By combining different types single structure, spheroids organoids can act basis microtissue – hybrid imitating specific phenotype containing tissuespecific extracellular matrix (ECM) components. review presents brief history culture. It describes main characteristics perspectives use “organ-on-a-chip” “microtissues” immune oncology solid tumors.

Language: Английский

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