Bacterial Bioaugmentation for Paracetamol Removal from Water and Sewage Sludge. Genomic Approaches to Elucidate Biodegradation Pathway

Published: Jan. 1, 2024

Wastewater treatment plants (WWTPs) are recognized as significant contributors of paracetamol (APAP) into the environment due to their limited ability degrade it. This study used a bioaugmentation strategy with Pseudomonas extremaustralis CSW01 and Stutzerimonas stutzeri CSW02 achieve APAP biodegradation in solution wide ranges temperature (10-40 oC) pH (5-9), reaching DT50 values < 1.5 hours 500 mg L-1 APAP. Bacterial strains also mineralized (<30%), but when forming consortia Mycolicibacterium aubagnense HPB1.1, mineralization significantly increased (up 74% 58% for CSW01+HPB1.1 CSW02+HPB1.1, respectively), decreasing only 1 9 days. Despite complete degradation its high mineralization, residual toxicity throughout process was observed. Three metabolites were identified (4-aminophenol, hydroquinone trans-2-hexenoic acid) that quickly disappeared, remained, indicating presence other non-detected intermediates. degraded 100% (50 kg-1) adsorbed on sewage sludge, 0.7 0.3 days, respectively, 15% mineralized. A genome-based analysis revealed amidases, deaminases, hydroxylases, dioxygenases enzymes involved biodegradation, possible metabolic pathway proposed.

Language: Английский

Modifications of the respiratory chain of Bacillus licheniformis as an alkalophilic and cyanide-degrading microorganism

Journal of Bioenergetics and Biomembranes, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 5, 2024

Abstract Bacillus licheniformis can use cyanide as a nitrogen source for its growth. However, it also carry out aerobic respiration in the presence of this compound, classic inhibitor mammalian cytochrome c oxidase, indicating that B. has branched respiratory chain with various terminal oxidases. Here, we studied modifications when cells were cultured Nutrient Broth, an alkaline medium ammonium, or cyanide. Then, measured oxygen consumption intact and membranes, enzyme activities, carried 1D 2D-BN-PAGE, followed by mass spectrometry analysis BN-PAGE bands associated NADH, NADPH, succinate dehydrogenase activities. We found cell growth was favored nutrient than In parallel, activity progressively decreased rich medium, lowest growing membranes contain dehydrogenases, proteomic detected nitrate reductase bc, caa3, aa3, bd complexes. The migrated molecular 375 kDa, association (115 kDa + 241 respectively). NADH forms aggregates different mass.

Language: Английский