Microbial paracetamol degradation involves a high diversity of novel amidase enzyme candidates

Water Research X, Journal Year: 2022, Volume and Issue: 16, P. 100152 - 100152

Published: Aug. 1, 2022

Pharmaceuticals are relatively new to nature and often not completely removed in wastewater treatment plants (WWTPs). Consequently, these micropollutants end up water bodies all around the world posing a great environmental risk. One exception this recalcitrant conversion is paracetamol, whose full degradation has been linked several microorganisms. However, genes corresponding proteins involved microbial paracetamol still elusive. In order improve our knowledge of pathway, we inoculated bioreactor with sludge hospital WWTP (Pharmafilter, Delft, NL) fed it as sole carbon source. Paracetamol was fully degraded without any lag phase enriched community investigated by metagenomic metatranscriptomic analyses, which demonstrated that very diverse. Dilution plating on paracetamol-amended agar plates yielded two

Language: Английский

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Mixed Contaminants: Occurrence, Interactions, Toxicity, Detection, and Remediation

Molecules, Journal Year: 2022, Volume and Issue: 27(8), P. 2577 - 2577

Published: April 16, 2022

The ever-increasing rate of pollution has attracted considerable interest in research. Several anthropogenic activities have diminished soil, air, and water quality led to complex chemical pollutants. This review aims provide a clear idea about the latest most prevalent pollutants such as heavy metals, PAHs, pesticides, hydrocarbons, pharmaceuticals—their occurrence various mixtures how several environmental factors influence their interaction. mechanism adopted by these contaminants form leading rise new class contaminants, thus resulting severe threats human health environment, also been exhibited. Additionally, this provides an in-depth vivo, vitro, trending biomarkers used for risk assessment identifies mixed even at very minute concentrations. Much importance given remediation technologies understand our current position handling can be improved. paper create awareness among readers ubiquitous simple ways tackle same.

Language: Английский

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Microbes as Resources to Remove PPCPs and Improve Water Quality

Microbial Biotechnology, Journal Year: 2025, Volume and Issue: 18(1)

Published: Jan. 1, 2025

ABSTRACT The inadequate removal of pharmaceuticals and personal care products (PPCPs) by traditional wastewater treatment plants (WWTPs) poses a significant environmental public health challenge. Residual PPCPs find their way into aquatic ecosystems, leading to bioaccumulation in biota, the dissemination antibiotic resistance genes (ARGs), contamination both water sources vegetables. These persistent pollutants can have negative effects on human health, ranging from development endocrine disruption. To mitigate these risks, there is growing interest exploiting microorganisms enzymes for bioremediation purposes. By harnessing metabolic capabilities microbial communities, be efficiently degraded, transformed, or sequestered systems. Additionally, communities exhibit remarkable adaptability resilience diverse PPCP contaminants, further underscoring potential as sustainable cost‐effective solutions treatment. This review explores promise an approach addressing complex challenges posed contamination, emphasising its safeguard integrity well‐being.

Language: Английский

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Characterization and biodegradation of paracetamol by biomass of Bacillus licheniformis strain PPY-2 isolated from wastewater

Rendiconti lincei. Scienze fisiche e naturali, Journal Year: 2023, Volume and Issue: 34(2), P. 491 - 501

Published: Feb. 23, 2023

Language: Английский

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Analyzing microbial communities and their biodegradation of multiple pharmaceuticals in membrane bioreactors

Applied Microbiology and Biotechnology, Journal Year: 2023, Volume and Issue: 107(17), P. 5545 - 5554

Published: July 12, 2023

Pharmaceuticals are of concern to our planet and health as they can accumulate in the environment. The impact these biologically active compounds on ecosystems is hard predict, information their biodegradation necessary establish sound risk assessment. Microbial communities promising candidates for pharmaceuticals such ibuprofen, but little known yet about degradation capacity multiple micropollutants at higher concentrations (100 mg/L). In this work, microbial were cultivated lab-scale membrane bioreactors (MBRs) exposed increasing a mixture six (ibuprofen, diclofenac, enalapril, caffeine, atenolol, paracetamol). Key players identified using combinatorial approach 16S rRNA sequencing analytics. community structure changed with pharmaceutical intake (from 1 100 mg/L) reached steady-state during incubation 7 weeks mg/L. HPLC analysis revealed fluctuating significant (30-100%) five pollutants (caffeine, paracetamol, enalapril) by an established stable mainly composed Achromobacter, Cupriavidus, Pseudomonas Leucobacter. By from MBR1 inoculum further batch culture experiments single (400 mg/L substrate, respectively), different consortia obtained each micropollutant. genera potentially responsible respective micropollutant identified, i.e. sp. Sphingobacterium caffeine Sphingomonas atenolol Klebsiella enalapril. Our study demonstrates feasibility cultivating capable degrading simultaneously highly concentrated MBRs identification specific pollutants. KEY POINTS: • Multiple removed communities. key main identified.

Language: Английский

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Discovery of a Ni2+-dependent heterohexameric metformin hydrolase

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: July 20, 2024

The biguanide drug metformin is a first-line blood glucose-lowering medication for type 2 diabetes, leading to its presence in the global environment. However, little known about fate of by microbial catabolism. Here, we characterize Ni

Language: Английский

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Studies on the bioaugmentation of Mycolicibacterium aubagnense HPB1.1 in aerobic granular sludge from a WWTP: adaptability of native prokaryotes and enhancement of paracetamol intermediate metabolites biodegradation

Environmental Technology & Innovation, Journal Year: 2025, Volume and Issue: 38, P. 104073 - 104073

Published: Feb. 6, 2025

Language: Английский

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Heterogeneous Bimetallic (La–Fe) Metal-Organic-Frameworks as an Efficient Bifunctional Catalyst for High-Performance Supercapacitors and Electrochemical Sensors

Journal of The Electrochemical Society, Journal Year: 2022, Volume and Issue: 169(10), P. 106521 - 106521

Published: Oct. 1, 2022

The thriving energy demand of the gradually increasing population and modernized life style requires development in storage devices for usage commercial electronic devices. synthesized La-Fe MOFs was found electrochemically active its electrochemical performances as battery-type electrode. In this work, an enzyme-free sensor based on La–Fe developed detection chlorambucil (CBL) paracetamol (PCT). Further modification can improve high surface area catalytic capability composites. Under optimal conditions, prepared has good linearity CBL PCT within range 0.05–265 μ M. limit (LOD) is 18.4 nM 15.6 nM, respectively. real sample analysis, stability selectivity sensors were analyzed. exhibited with higher charge capacity. specific capacity 171 C g −1 at a constant applied current density 1.5 A 1 M KOH. addition Fe to La-MOFs helped overcome issues achieve better performance. It be promising candidate device meet global demand.

Language: Английский

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Pharmaceutical Pollutants: Ecotoxicological Impacts and the Use of Agro-Industrial Waste for Their Removal from Aquatic Environments

Journal of Xenobiotics, Journal Year: 2024, Volume and Issue: 14(4), P. 1465 - 1518

Published: Oct. 15, 2024

Medicines are pharmaceutical substances used to treat, prevent, or relieve symptoms of different diseases in animals and humans. However, their large-scale production use worldwide cause release the environment. Pharmaceutical molecules currently considered emerging pollutants that enter water bodies due inadequate management, affecting quality generating adverse effects on aquatic organisms. Hence, alternatives for pharmaceuticals removal from have been sought; among them, agro-industrial wastes has proposed, mainly because its high availability low cost. This review highlights ecotoxicological related presence environments analyzes 94 investigations, 2012 2024, 17 antibiotics, highlighting sulfamethoxazole as most reported, well 6 non-steroidal anti-inflammatory drugs (NSAIDs) such diclofenac ibuprofen, 27 with pharmacological activities. The these was evaluated using wheat straw, mung bean husk, bagasse, bamboo, olive stones, rice pinewood, others. On average, 60% were transformed into biochar be a biosorbents removal. diversity experimental conditions studies makes it difficult stablish which waste greatest capacity; therefore, this review, drug mass rate (DMRR) calculated, parameter comparative purposes. Almond shell-activated showed highest antibiotics (1940 mg/g·h), while cork powder (CP) (10,420 mg/g·h) NSAIDs. Therefore, scientific evidence demonstrates is promising alternative substances.

Language: Английский

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An Overview of Degradation Strategies for Amitriptyline

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(7), P. 3822 - 3822

Published: March 29, 2024

Antidepressant drugs play a crucial role in the treatment of mental health disorders, but their efficacy and safety can be compromised by drug degradation. Recent reports point to several found concentrations ranging from limit detection (LOD) hundreds ng/L wastewater plants around globe; hence, antidepressants considered emerging pollutants with potential consequences for human wellbeing. Understanding implementing effective degradation strategies are essential not only ensure stability potency these medications also safe disposal line current environment remediation goals. This review provides an overview pathways amitriptyline, typical tricyclic antidepressant drug, exploring chemical routes such as oxidation, hydrolysis, photodegradation. Connex issues stability-enhancing approaches through formulation packaging considerations, regulatory guidelines, quality control measures briefly noted. Specific case studies amitriptyline forecast future perspectives challenges this field, helping researchers pharmaceutical manufacturers provide guidelines most employed minimal environmental impact.

Language: Английский

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