Batch studies on the biodegradation of paracetamol and 1,4-hydroquinone by novel bacterial strains isolated from extreme environmental samples and the identification of candidate catabolic genes

Applied Water Science, Journal Year: 2024, Volume and Issue: 14(9)

Published: Aug. 13, 2024

The emerging pollutant paracetamol (APAP) is one of the most prescribed drugs worldwide. In addition, APAP and its main metabolites, namely, 4-aminophenol (4-AP), hydroquinone (H2Q), benzoquinone (BQ), 2,5-dihydroxy-1,4-benzoquinone (2,5-OH-BQ), among others, are frequently detected in wastewater treatment plants (WWTPs) influents, effluents, environment. Thus, continuous release into environment, especially aquatic environments, a source general concern. Six APAP-degrading bacterial strains were isolated from two mine samples Iberian Pyrite Belt (Lousal Poderosa mines). Mycolicibacterium aubagnense HPB1.1, which was using enrichment cultures sample presence H2Q as sole carbon source, also showed biodegrading capabilities. Pure this strain degraded 34.3 mg L−1 5 days 9.4 4 days. Interestingly, BQ 2,5-OH-BQ metabolites resulting abiotic degradation, but these compounds removed strain's cultures. Furthermore, M. HPB1.1 whole-genome sequenced, encoded proteins aligned with enzymes bacteria recovered databases literature aiming to identify candidate catabolic genes. Putative amidases, deaminases, hydroxylases, dioxygenases, responsible for degradation by strain, identified similarity, corroborating ability transform intermediate metabolite less toxic metabolic due their capacity break aromatic ring molecules.

Language: Английский

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Bacterial bioaugmentation for paracetamol removal from water and sewage sludge. Genomic approaches to elucidate biodegradation pathway

Journal of Hazardous Materials, Journal Year: 2024, Volume and Issue: 480, P. 136128 - 136128

Published: Oct. 12, 2024

Wastewater treatment plants (WWTPs) are recognized as significant contributors of paracetamol (APAP) into the environment due to their limited ability degrade it. This study used a bioaugmentation strategy with Pseudomonas extremaustralis CSW01 and Stutzerimonas stutzeri CSW02 achieve APAP biodegradation in solution wide ranges temperature (10-40 °C) pH (5-9), reaching DT

Language: Английский

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BiVO4-Based Photocatalysts for the Degradation of Antibiotics in Wastewater: Calcination Role after Solvothermal Synthesis

Catalysts, Journal Year: 2024, Volume and Issue: 14(8), P. 474 - 474

Published: July 25, 2024

BiVO4 is an important n-type semiconductor used in photocatalysis due to its high capacity absorb solar light the 400–700 nm range, abundance, chemical stability, non-toxicity, and low cost. However, research on physicochemical modifications increase catalytic activity via simple procedures limited. In this work, influence of different synthesis parameters, such as calcination temperatures or silver doping, structural characteristic BiVO4-based photocatalysts their photocatalytic performance degrading sulfamethoxazole from aqueous solution under blue-LED irradiation was evaluated. were synthesized using a solvothermal method. The monoclinic phase (m-s) successfully kept stable even after thermal treatments at 300, 450, 600 °C corresponding doping. bandgap 2.40 eV average particle size 18 catalyst treated 300 seems be key. Afte Ag/BiVO4 photocatalyst optimal found temperature (300 °C) showed best behavior.

Language: Английский

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Identification of microbial communities and their removal efficiency of multiple pharmaceutical micropollutants combined in Membrane-Bioreactors

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: April 11, 2023

Abstract Pharmaceuticals are of concern to our planet and health as they can accumulate in the environment. The impact these biologically active compounds on ecosystems is hard predict information their biodegradation necessary establish sound risk assessment. Microbial communities promising candidates for pharmaceuticals such ibuprofen, but little known yet about degradation-capacity multiple micropollutants at higher concentrations (100 mg/L). In this work, microbial were cultivated lab-scale Membrane Bioreactors (MBRs) exposed increasing a mixture six (ibuprofen, diclofenac, enalapril, caffeine, atenolol, paracetamol). Key players identified using combinatorial approach 16S rRNA sequencing analytics. community structure changed with pharmaceutical intake (from 1 mg/L 100 mg/L) reached steady-state during incubation 7 weeks mg/L. HPLC analysis revealed fluctuating significant degradation (30-100%) five pollutants (caffeine, paracetamol, enalapril) by an established stable mainly composed Achromobacter , Cupriavidus Pseudomonas Leucobacter . By from MBR1 inoculum further batch culture experiments single (400 substrate, respectively), different consortia obtained each micropollutant. genera potentially responsible respective micropollutant identified, i.e. sp. Sphingobacterium caffeine Sphingomonas Klebsiella enalapril. Our study demonstrates feasibility cultivating capable degrading simultaneously highly concentrated MBRs identification specific pollutants. Graphical abstract

Language: Английский

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Microbial paracetamol degradation involves a high diversity of novel amidase enzyme candidates

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2022, Volume and Issue: unknown

Published: May 5, 2022

Abstract Pharmaceuticals are relatively new to nature and often not completely removed in wastewater treatment plants (WWTPs). Consequently, these micropollutants end up water bodies all around the world posing a great environmental risk. One exception this recalcitrant conversion is paracetamol, whose full degradation has been linked several microorganisms. However, genes corresponding proteins involved microbial paracetamol still elusive. In order improve our knowledge of pathway, we inoculated bioreactor with sludge hospital WWTP (Pharmafilter, Delft, NL) fed it as sole carbon source. Paracetamol was fully degraded without any lag phase enriched community investigated by metagenomic metatranscriptomic analyses, which demonstrated that very diverse. Dilution plating on paracetamol-amended agar plates yielded two Pseudomonas sp. isolates: fast-growing 200 mg/L approximately 10 hours while excreting dark brown component medium, slow-growing obvious intermediates more than 90 days. Each contained different highly-expressed amidase (31% identity each other). These were detected metagenome suggesting other as-yet uncharacterized amidases may be responsible for first biodegradation step paracetamol. Uncharacterized deaminase encoding dioxygenase enzymes catabolism aromatic compounds amino acids most likely candidates based their high expression levels spp. genomes. Furthermore, cross-feeding between members might have occurred efficiently degrade its bioreactor. This study increases about ongoing evolution towards pharmaceuticals points large diversity (amidase) metabolism WWTPs. Highlights activated from wastewater. Low concentrations diverse community. dominated cultures concentration. probably degrading Deaminases dioxygenases transformation products.

Language: Английский

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Bioremediation of Acetaminophen and Hydroxychloroquine by Kosakonia cowanii JCM 10956(T) with ecotoxicity studies

Research Square (Research Square), Journal Year: 2023, Volume and Issue: unknown

Published: May 17, 2023

Abstract Acetaminophen and hydroxychloroquine are widely used drugs during COVID situations. Residual concentrations of acetaminophen have been detected in pharmaceutical industry wastewater, effluent treatment plants, surface water. The present study was carried out on the bioremediation (paracetamol) by using bacterial isolate Kosakonia cowanii JCM 10956(T) (GenBank: OQ733302.1). Identification done 16S rRNA sequencing technique. LC50 values for bacteria were determined as 2186.70 1735.13 ppm, respectively. Isolate found to degrade (1500 ppm) into hydroquinone after five days incubation with an 81% biodegradation rate. Hydroxychloroquine (1000 be degraded oxalic acid 7-chloroquinoline-4-amine 4-aminoquinoline-7-ol intermediates. After 15 incubation, 60% degraded. followed a first-order kinetic model rate constant 0.339 d − 1 0.0618 , Half-lives 2.05 11.2 days, Based analytical techniques UV-visible spectra, HPLC, mass proton nuclear magnetic spectroscopy (1H NMR) studies, biodegradative metabolites identified. Ecotoxicological testing parent drug degradative product algal inhibition shrimp lethality assays. acetaminophen, hydroquinone, has more toxicity less against compared drug. Whereas product, Industrial applications metal leaching role will give new insight bioconversion expired paracetamol value-added products.

Language: Английский

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Studies on Biodegradation of Acetaminophen by Bacillus subtilis subsp. subtilis NCIB 3610(T)

Current World Environment, Journal Year: 2023, Volume and Issue: 18(1), P. 155 - 163

Published: April 29, 2023

Acetaminophen (paracetamol) has been classified as one of the emerging organic pollutants due to its entrance into water bodies. Removal pharma micropollutants using microorganisms or synthetic systems from environment is desirable. In this study, acetaminophen degrading microorganism was isolated Pharmaceutical industrial wastewater. Identification isolate carried out morphological, biochemical, and 16s rRNA sequencing. Bacillus subtilis subsp. NCIB 3610(T) showed 73.2% degradation paracetamol (2500 ppm) based on colorimetric reverse phase high performance liquid chromatography analysis. Based Computer Assisted Kinetic Evaluation (CAKE) program, it can be concluded that strain exhibits Simple first -order model (SFO). The degradative product identified 4-aminophenol High Resolution Mass Spectrometry method. chemotaxis assay reported understudy found suitable for bioaugmentation purposes.

Language: Английский

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Discovery of a unique Ni2+-dependent heterohexameric metformin hydrolase

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 31, 2024

The biguanide drug metformin is prescribed worldwide as a first-line blood glucose-lowering medication for type 2 diabetes, leading to its presence in the environment around world. Despite mounting evidence indicating that metabolic interactions of with environmental or intestinal microbes affect ecological and human health, little known about fate by microbial catabolism. Here, we characterized Ni²⁺-dependent bacterial enzyme catalyzes hydrolysis form guanylurea dimethylamine. hydrolase MetCaCb heterohexamer an uneven α₂β₄ stoichiometry. Both subunits are from arginase protein family, members which typically homomultimers. Either subunit alone catalytically inactive, but together they work active highly specific metformin. crystal structure complex clearly shows coordination binuclear metal cluster only MetCa, following geometry typical family enzymes. A unique pseudoenzyme MetCb, evolved without cluster, contributes activity binder cognate. An in-silico search functional assay led discovery group MetCaCb-like pairs exhibiting environment. Our findings not establish genetic biochemical foundation catabolism also provide new insights into adaption ancient proteins toward newly occurred substrate.

Language: Английский

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Bacterial Bioaugmentation for Paracetamol Removal from Water and Sewage Sludge. Genomic Approaches to Elucidate Biodegradation Pathway

Published: Jan. 1, 2024

Wastewater treatment plants (WWTPs) are recognized as significant contributors of paracetamol (APAP) into the environment due to their limited ability degrade it. This study used a bioaugmentation strategy with Pseudomonas extremaustralis CSW01 and Stutzerimonas stutzeri CSW02 achieve APAP biodegradation in solution wide ranges temperature (10-40 oC) pH (5-9), reaching DT50 values < 1.5 hours 500 mg L-1 APAP. Bacterial strains also mineralized (<30%), but when forming consortia Mycolicibacterium aubagnense HPB1.1, mineralization significantly increased (up 74% 58% for CSW01+HPB1.1 CSW02+HPB1.1, respectively), decreasing only 1 9 days. Despite complete degradation its high mineralization, residual toxicity throughout process was observed. Three metabolites were identified (4-aminophenol, hydroquinone trans-2-hexenoic acid) that quickly disappeared, remained, indicating presence other non-detected intermediates. degraded 100% (50 kg-1) adsorbed on sewage sludge, 0.7 0.3 days, respectively, 15% mineralized. A genome-based analysis revealed amidases, deaminases, hydroxylases, dioxygenases enzymes involved biodegradation, possible metabolic pathway proposed.

Language: Английский

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Binary and ternary interactions of Gantrez™ AN-169 with epinephrine and copper (II) in NaCl aqueous solution: Thermodynamic approach and simulations in real conditions

Journal of Molecular Liquids, Journal Year: 2024, Volume and Issue: unknown, P. 126680 - 126680

Published: Dec. 1, 2024

Language: Английский

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