Upregulation of NR2B Subunits of NMDA Receptors in the Lateral Parabrachial Nucleus Contributes to Chronic Pancreatitis Pain

CNS Neuroscience & Therapeutics, Journal Year: 2025, Volume and Issue: 31(3)

Published: Feb. 28, 2025

Chronic pancreatitis (CP) is a localized or diffuse chronic progressive inflammation of the pancreas that can be caused by variety factors and characterized abdominal pain. However, underlying mechanisms are poorly understood. Increasing evidence suggests central sensitization plays crucial role in development visceral pain, but precise neural processing remain unclear. CP was induced using repeated intraperitoneal injections caerulein mice. Neurospecific anterograde tracing achieved herpes simplex virus type 1 (HSV-1). Fiber photometry used to assess neuronal activity. Optogenetic, chemogenetic, pharmacological approaches were applied manipulate lateral parabrachial nucleus (LPB) glutamatergic neurons. The withdrawal threshold (AWT) measured evaluate A glutamate sensor detect release LPB. In present study, we demonstrated neurons LPB activated mice, leading Notably, increased LPB, primarily mediates pain binding N-methyl-D-aspartate (NMDA) receptor rather than α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptors. Specifically, this process involves N-Methyl-D-Aspartate Receptor Subunit 2B (NR2B) This study identified NR2B subunits NMDA receptors as playing critical regulation

Language: Английский

Involvement of the parabrachial nucleus in emergence from general anesthesia

Frontiers in Neuroscience, Journal Year: 2024, Volume and Issue: 18

Published: Dec. 11, 2024

The parabrachial nucleus (PBN), located in the dorsolateral pons, is involved many important biological functions, such as sensory signaling, feeding, defensive behaviors, fear, anxiety, and sleep–wake cycles. General anesthesia shares classical feature of reversible loss consciousness with natural sleep, accumulating evidence has indicated that general behaviors share some common underlying neural mechanism. In recent years, emerging studies have investigated involvement PBN emergence from anesthesia, but divergence exists terms different types anesthetics or durations treatment same group anesthetics. Here, we reviewed current literature summarized about contribution to anesthesia.

Language: Английский