Targeting corticotropin-releasing hormone receptor type 1 (Crhr1) neurons: validating the specificity of a novel transgenic Crhr1-FlpO mouse DOI Creative Commons
M P Hardy, Yuncai Chen, Tallie Z. Baram

et al.

Brain Structure and Function, Journal Year: 2024, Volume and Issue: 230(1)

Published: Dec. 18, 2024

Corticotropin-releasing hormone (CRH) signaling through its cognate receptors, CRHR1 and CRHR2, contributes to diverse stress-related functions in the mammalian brain. Whereas CRHR2 is predominantly expressed choroid plexus blood vessels, abundantly neurons discrete brain regions, including neocortex, hippocampus nucleus accumbens. Activation of influences motivated behaviors, emotional states, learning memory. However, it unknown whether alterations contribute aberrant behaviors observed, for example, stressful contexts. These questions require tools manipulate selectively. Here we describe validate a novel Crhr1-FlpO mouse. Using bacterial artificial chromosome (BAC) transgenesis, engineered transgenic mouse that expresses FlpO recombinase CRHR1-expressing cells. We used two independent methods assess specificity First, injected mice with Flp-dependent viruses expressing fluorescent reporter molecules. Additionally, crossed molecules were identified using immunocytochemistry visualized via confocal microscopy several regions which expression function established. Expression viral constructs was highly specific cells all examined (over 90% co-localization). In accord, robust observed mouse, recapitulating endogenous expression. The enables selective genetic access within When combined Cre-lox or site-specific recombinases, facilitates intersectional manipulations neurons.

Language: Английский

Few but Not Futile: A Sparse Nucleus Accumbens Corticotropin-Releasing Factor Population Regulates Reward Learning DOI
Alison V. Roland

Biological Psychiatry, Journal Year: 2025, Volume and Issue: 97(6), P. 561 - 562

Published: Feb. 17, 2025

Language: Английский

Citations

0
Perifornical UCN3 Neurons Regulate Overeating-Induced Weight Gain DOI
Shanshan Lu, X Zhang, Wanqi Chen

et al.

Neuroscience Bulletin, Journal Year: 2025, Volume and Issue: unknown

Published: April 19, 2025

Language: Английский

Citations

0
Upregulation of NR2B Subunits of NMDA Receptors in the Lateral Parabrachial Nucleus Contributes to Chronic Pancreatitis Pain DOI Creative Commons
Junchen Wu,

Weihong Kuang,

Zhengyan Zhu

et al.

CNS Neuroscience & Therapeutics, Journal Year: 2025, Volume and Issue: 31(3)

Published: Feb. 28, 2025

Chronic pancreatitis (CP) is a localized or diffuse chronic progressive inflammation of the pancreas that can be caused by variety factors and characterized abdominal pain. However, underlying mechanisms are poorly understood. Increasing evidence suggests central sensitization plays crucial role in development visceral pain, but precise neural processing remain unclear. CP was induced using repeated intraperitoneal injections caerulein mice. Neurospecific anterograde tracing achieved herpes simplex virus type 1 (HSV-1). Fiber photometry used to assess neuronal activity. Optogenetic, chemogenetic, pharmacological approaches were applied manipulate lateral parabrachial nucleus (LPB) glutamatergic neurons. The withdrawal threshold (AWT) measured evaluate A glutamate sensor detect release LPB. In present study, we demonstrated neurons LPB activated mice, leading Notably, increased LPB, primarily mediates pain binding N-methyl-D-aspartate (NMDA) receptor rather than α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptors. Specifically, this process involves N-Methyl-D-Aspartate Receptor Subunit 2B (NR2B) This study identified NR2B subunits NMDA receptors as playing critical regulation

Language: Английский

Citations

0
Special Issue Celebrating the 25th Anniversary of the Institute of Neuroscience, CAS DOI Creative Commons

Ting Lv,

Yefei Li, Fei Dong

et al.

Neuroscience Bulletin, Journal Year: 2024, Volume and Issue: 40(11), P. 1599 - 1601

Published: Nov. 1, 2024

Language: Английский

Citations

0
Targeting corticotropin-releasing hormone receptor type 1 (Crhr1) neurons: validating the specificity of a novel transgenic Crhr1-FlpO mouse DOI Creative Commons
M P Hardy, Yuncai Chen, Tallie Z. Baram

et al.

Brain Structure and Function, Journal Year: 2024, Volume and Issue: 230(1)

Published: Dec. 18, 2024

Corticotropin-releasing hormone (CRH) signaling through its cognate receptors, CRHR1 and CRHR2, contributes to diverse stress-related functions in the mammalian brain. Whereas CRHR2 is predominantly expressed choroid plexus blood vessels, abundantly neurons discrete brain regions, including neocortex, hippocampus nucleus accumbens. Activation of influences motivated behaviors, emotional states, learning memory. However, it unknown whether alterations contribute aberrant behaviors observed, for example, stressful contexts. These questions require tools manipulate selectively. Here we describe validate a novel Crhr1-FlpO mouse. Using bacterial artificial chromosome (BAC) transgenesis, engineered transgenic mouse that expresses FlpO recombinase CRHR1-expressing cells. We used two independent methods assess specificity First, injected mice with Flp-dependent viruses expressing fluorescent reporter molecules. Additionally, crossed molecules were identified using immunocytochemistry visualized via confocal microscopy several regions which expression function established. Expression viral constructs was highly specific cells all examined (over 90% co-localization). In accord, robust observed mouse, recapitulating endogenous expression. The enables selective genetic access within When combined Cre-lox or site-specific recombinases, facilitates intersectional manipulations neurons.

Language: Английский

Citations

0