Lipid Nanoparticle-Mediated Oip5-as1 Delivery Preserves Mitochondrial Function in Myocardial Ischemia/Reperfusion Injury by Inhibiting the p53 Pathway

ACS Applied Materials & Interfaces, Journal Year: 2024, Volume and Issue: 16(45), P. 61565 - 61582

Published: Nov. 1, 2024

Myocardial ischemia/reperfusion (MI/R) injury, a major contributor to poor prognosis in patients with acute myocardial infarction, currently lacks effective therapeutic strategies clinical practice. The long noncoding RNA (lncRNA) Oip5-as1 can regulate various cellular processes, such as cell proliferation, differentiation, and survival. may have potential target for MI/R injury its upregulated expression has been associated reduced infarct size improved cardiac function animal models, although how effectively safely overexpress vivo remains unclear. Lipid nanoparticles (LNPs) are versatile technology targeted drug delivery numerous applications. Herein, we aimed assess the efficacy safety of LNPs coloaded cardiomyocyte-specific binding peptide (LNP@Oip5-as1@CMP) murine model injury. To achieve this, LNP@Oip5-as1@CMP was synthesized via ethanol injection method. structural components were physicochemically analyzed. A hypoxia/reoxygenation (H/R) HL-1 cells coronary artery ligation mice used simulate Our results demonstrated that designed cardiomyocyte targeting efficient successfully synthesized. In cells, treatment significantly mitochondrial apoptosis caused by H/R model, intravenous administration decreased function. Mechanistic investigations revealed inhibited p53 signaling pathway. However, cardioprotective effects abrogated administrating Nutlin-3a, activator. Furthermore, no signs organ damage detected after injection. study reveals mitigating These findings pave way advanced treatments cardiovascular diseases, emphasizing promise lncRNA-based therapies.

Language: Английский

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Comprehensive Quality Analysis of Conventional and Novel Biomarkers in Diagnosing and Predicting Prognosis of Coronary Artery Disease, Acute Coronary Syndrome, and Heart Failure, a Comprehensive Literature Review

Journal of Cardiovascular Translational Research, Journal Year: 2024, Volume and Issue: 17(6), P. 1258 - 1285

Published: July 12, 2024

Language: Английский

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LncRNA NEAT1 sponges miR-214-3p to promote osteoblast differentiation through regulating the PI3K/AKT/mTOR pathway in aortic valve calcification

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: April 21, 2025

Calcific aortic valve disease (CAVD) is the common valvular associated with significant morbidity and mortality. Dysregulation of long non-coding RNA (lncRNA) has been implicated in pathogenesis CAVD. This study aims to investigate role NEAT1 CAVD pathogenesis. NEAT1, miR-214-3p mRNA expressions were determined by qRT-PCR. Protein detected Western blotting. Mineralized bone matrix formation was assessed Alizarin Red staining. The osteogenic phenotype evaluated alkaline phosphatase activity assay. Dual-luciferase assays employed confirm binding interactions between miR-214-3p, PTEN. up-regulated calcific valves after induction interstitial cells (VICs). could act as a positive regulator differentiation repressing thereby promote expression osteoblast-specific markers. Mechanistically, we identified PTEN direct target miR-214-3p. regulate PI3K/AKT/mTOR pathway participate differentiation. Importantly, directly interact change efficiently reverse induced NEAT1. Thus, positively regulated activated autophagy through sponging promoted pathway. In conclusion, elucidates vital function miRNA sponge pathogenesis, sheds new light on lncRNA-directed diagnostic therapeutic strategies for

Language: Английский

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The key role of miRNA in syndromic and sporadic forms of ascending aortic aneurysms as biomarkers and targets of novel therapeutic strategies

Frontiers in Genetics, Journal Year: 2024, Volume and Issue: 15

Published: Feb. 21, 2024

Increasing evidence shows that epigenetics also plays a key role in regulating the pathogenetic mechanism of all types aortic aneurysms. It is well-known epigenetic factors modulate gene expression. This appears to be interest especially knowing relevance genetic susceptibility and complex pathophysiology aneurysms, sporadic forms; fact, latter are result close interaction between modifiable lifestyle (i.e., nutrition, smoking, infections, use drugs, alcohol, sedentary lifestyle, etc.). Epigenetic include DNA methylation, post-translational histone modifications, non-coding RNA. Here, our attention focused on miRNA syndromic forms thoracic They could both biomarkers targets novel therapeutic strategies.

Language: Английский

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Genetic factors and management strategies in aortic health: a literature review of inherited aortopathy

Annals of Medicine and Surgery, Journal Year: 2024, Volume and Issue: 87(2), P. 598 - 615

Published: Dec. 12, 2024

Language: Английский

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