Nano Convergence,
Journal Year:
2023,
Volume and Issue:
10(1)
Published: June 9, 2023
The
understanding
of
the
endocytosis
process
internalized
nanomedicines
through
membrane
biomarker
is
essential
for
development
molecular-specific
nanomedicines.
In
various
recent
reports,
metalloproteases
have
been
identified
as
important
markers
during
metastasis
cancer
cells.
particular,
MT1-MMP
has
provoked
concern
due
to
its
protease
activity
in
degradation
extracellular
matrix
adjacent
tumors.
Thus,
current
work,
we
applied
fluorescent
Au
nanoclusters
which
present
strong
resistance
chemical
quenching
investigation
MT1-MMP-mediated
endocytosis.
We
synthesized
protein-based
nanocluster
(PAuNC)
and
MT1-MMP-specific
peptide
was
conjugated
with
PAuNC
(pPAuNC)
monitoring
protease-mediated
fluorescence
capacity
pPAuNC
investigated
intracellular
uptake
subsequently
confirmed
by
a
co-localization
analysis
using
confocal
microscopy
molecular
competition
test.
Furthermore,
change
lipophilic
network
after
an
event
pPAuNC.
identical
did
not
occur
bare
PAuNC.
By
classification
branched
between
organelles
at
nanoscale,
image-based
cell
organelle
networking
allowed
evaluation
nanoparticle
internalization
impaired
cellular
components
accumulation
single-cell
level.
Our
analyses
suggest
methodology
achieve
better
mechanism
nanoparticles
enter
Biomacromolecules,
Journal Year:
2023,
Volume and Issue:
24(9), P. 4303 - 4315
Published: Aug. 16, 2023
Stimuli-responsive
polymer
nanocarriers,
capable
of
exploiting
subtle
changes
in
the
tumor
microenvironment
for
controlled
drug
release,
have
gained
significant
attention
cancer
therapy.
Notably,
NAD(P)H:
quinone
oxidoreductase
1
(NQO1),
found
to
be
upregulated
various
solid
tumors,
represents
a
promising
therapeutic
target
due
its
effective
capability
enzymatically
reduce
trimethyl-locked
(TML)
benzoquinone
structures
physiological
condition.
In
this
study,
novel
redox-sensitive
carbonate
monomer,
MTC,
was
synthesized,
and
amphiphilic
block
copolymers
were
prepared
through
ring-opening
polymerization.
By
successfully
self-assembling
poly(ethylene
glycol)-b-PMTC
micelles,
model
doxorubicin
(DOX)
encapsulated
with
high
efficiency.
The
micelles
exhibited
redox-responsive
behavior,
leading
rapid
release.
vitro
assessments
confirmed
their
excellent
biocompatibility
hemocompatibility.
Furthermore,
inhibition
NQO1
enzyme
reduced
release
NQO1-overexpressed
cells
but
not
control
cells,
resulting
decreased
cytotoxicity
presence
inhibitors.
Overall,
study
showcases
potential
MTC-based
polycarbonate
achieve
targeted
specific
enzyme-mediated
microenvironment.
Therefore,
self-assembly
polymers
into
nanomicelles
holds
immense
promise
as
intelligent
nanocarriers
delivery
applications.
Oncology Research Featuring Preclinical and Clinical Cancer Therapeutics,
Journal Year:
2024,
Volume and Issue:
33(2), P. 407 - 420
Published: June 4, 2024
Sunitinib
resistance
is
a
major
challenge
in
advanced
renal
cell
carcinoma
(RCC).
Clinically,
elucidating
the
underlying
mechanisms
and
developing
practical
countermeasures
for
sunitinib
RCC
desirable.
In
previous
studies,
we
found
that
circAGAP1
expression
was
significantly
upregulated
clear
(ccRCC)
strongly
associated
with
poor
prognosis.
However,
role
of
ccRCC
remains
unclear.
We
used
public
databases
bioinformatics
analysis
to
identify
binding
targets
circAGAP1.
Additionally,
effects
on
proliferation,
clonogenesis,
apoptosis,
migration
cells
were
analyzed
using
quantitative
real-time
PCR,
counting
kit-8
assays,
apoptosis
colony
formation
assays.
Furthermore,
RNA
immunoprecipitation,
dual-luciferase
reporter,
fluorescence
situ
hybridization
assays
explore
molecular
mechanism.
this
study,
exhibited
higher
sunitinib-sensitive
inhibited
after
treatment.
Mechanical
studies
revealed
regulated
target
platelet-derived
growth
factor
receptor
by
acting
as
microRNA
sponge
suppresses
miR-149-5p,
miR-455-5p,
miR-15a-5p
simultaneously.
Overexpression
these
three
miRNAs
reversed
circAGAP1-mediated
sensitivity
ccRCC.
summary,
our
findings
indicate
may
serve
promising
biomarker
predict
sensibility
therapeutic
Nano Convergence,
Journal Year:
2023,
Volume and Issue:
10(1)
Published: June 9, 2023
The
understanding
of
the
endocytosis
process
internalized
nanomedicines
through
membrane
biomarker
is
essential
for
development
molecular-specific
nanomedicines.
In
various
recent
reports,
metalloproteases
have
been
identified
as
important
markers
during
metastasis
cancer
cells.
particular,
MT1-MMP
has
provoked
concern
due
to
its
protease
activity
in
degradation
extracellular
matrix
adjacent
tumors.
Thus,
current
work,
we
applied
fluorescent
Au
nanoclusters
which
present
strong
resistance
chemical
quenching
investigation
MT1-MMP-mediated
endocytosis.
We
synthesized
protein-based
nanocluster
(PAuNC)
and
MT1-MMP-specific
peptide
was
conjugated
with
PAuNC
(pPAuNC)
monitoring
protease-mediated
fluorescence
capacity
pPAuNC
investigated
intracellular
uptake
subsequently
confirmed
by
a
co-localization
analysis
using
confocal
microscopy
molecular
competition
test.
Furthermore,
change
lipophilic
network
after
an
event
pPAuNC.
identical
did
not
occur
bare
PAuNC.
By
classification
branched
between
organelles
at
nanoscale,
image-based
cell
organelle
networking
allowed
evaluation
nanoparticle
internalization
impaired
cellular
components
accumulation
single-cell
level.
Our
analyses
suggest
methodology
achieve
better
mechanism
nanoparticles
enter
