The emergence of nanovaccines as a new paradigm in virological vaccinology: a review DOI Creative Commons
Chittaranjan Baruah, Pankaj Das, Papari Devi

et al.

Exploration of Immunology, Journal Year: 2023, Volume and Issue: unknown, P. 361 - 383

Published: Aug. 31, 2023

Vaccination has made an enormous contribution to global health. Treatment resistance for infectious diseases is growing quickly, and chemotherapeutic toxicity in cancer means that vaccines must be right away save humanity. But subunit vaccinations alone don’t give enough strong long-lasting protection against infections can kill. Nanoparticle (NP)-based delivery vehicles, such as dendrimers, liposomes, micelles, virosomes, nanogels, microemulsions, offer interesting ways get around the problems with traditional vaccine adjuvants. The nanovaccines (50–250 nm size) are most efficient terms of tissue targeting, staying bloodstream a long time. Nanovaccines improve antigen presentation, targeted delivery, stimulation body’s innate immune system, T-cell response without putting people at risk. This help fight cancers. Also, very helpful making treatments use immunotherapy. So, this review highlights various types NPs used techniques have worked new paradigm viral vaccinology diseases. It gives full rundown current NP-based vaccines, their potential adjuvants, they delivered cells. In future, best will try more logically designed, antigens them, fully functionalized, given people.

Language: Английский

T4 Phage Displaying Dual Antigen Clusters Against H3N2 Influenza Virus Infection DOI Creative Commons
Shenglong Liu,

Mengzhou Lin,

Xin Zhou

et al.

Vaccines, Journal Year: 2025, Volume and Issue: 13(1), P. 70 - 70

Published: Jan. 13, 2025

Background: The current H3N2 influenza subunit vaccine exhibits weak immunogenicity, which limits its effectiveness in preventing and controlling virus infections. Methods: In this study, we aimed to develop a T4 phage-based nanovaccine designed enhance the immunogenicity of two antigens by displaying HA1 M2e on each phage nanoparticle. Specifically, fused Soc protein with antigen Hoc antigen, assembling them onto that lacks proteins (Soc−Hoc−T4), thereby constructing concurrently presents both antigens. Results: analysis optical density target bands indicated particle could display approximately 179 68 molecules. Additionally, animal experiments demonstrated nanoparticle dual clusters induced stronger specific immune response, higher antibody titers, more balanced Th1/Th2 enhanced CD4+ CD8+ T cell effects compared immunization molecules alone. Importantly, mice immunized achieved full protection (100% protection) against virus, highlighting robust protective efficacy. Conclusions: summary, our findings indicate particles based exhibit ideal effects, providing promising strategy for development vaccines various viruses beyond influenza.

Language: Английский

Citations

1
Immunoinformatic evaluation for the development of a potent multi-epitope vaccine against bacterial vaginosis caused by Gardnerella vaginalis DOI Creative Commons

Hamid Motamedi,

Saeed Shoja, Maryam Abbasi

et al.

PLoS ONE, Journal Year: 2025, Volume and Issue: 20(2), P. e0316699 - e0316699

Published: Feb. 27, 2025

Background Bacterial vaginosis (BV) is the most common vaginal dysbiosis in fertile women, which associated with side effects including risk of premature birth. Gardnerella vaginalis ( G . ) a facultative anaerobic bacillus known as main pathogen responsible for BV. In this study, using bioinformatics and immunoinformatics methods, multi-epitope vaccine optimal population coverage against BV caused by was designed. Methods Amino acid sequences two important virulence factors (Vaginolysin Sialidase) were retrieved from NCBI UniProt databases. At first, three online servers ABCpred, BCPREDS LBtope used to predict linear B-cell epitopes (BCEs) IEDB server T cells. Then antigenicity, toxicity, allergenicity evaluated tools. After modeling three-dimensional (3D) structure Robetta Server, molecular docking dynamics performed. Finally, immune simulation silico cloning considered effective design production strategy. Results total, six BCEs, eight CD4 + seven CD8 selected. The designed non-allergenic non-toxic showed high levels antigenicity immunogenicity. 3D predicted, it refined validated, resulted an optimized model Z-score -7.4. Molecular revealed stable strong binding interactions. results immunity significant increase immunoglobulins, higher IFN-γ IL-2. Conclusion According findings, candidate has structural features. It also potential stimulate long-term host, but wet-lab validation needed justify it.

Language: Английский

Citations

1
Self-adjuvant multiepitope nanovaccine based on ferritin induced long-lasting and effective mucosal immunity against H3N2 and H1N1 viruses in mice DOI
Jiaojiao Nie,

Yong-Fei Zhou,

Fan Ding

et al.

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 259, P. 129259 - 129259

Published: Jan. 6, 2024

Language: Английский

Citations

5
Induction of Enhanced Stem-directed Neutralizing Antibodies by HA2-16 Ferritin nanoparticles with H3 Influenza Virus Boost DOI Creative Commons
Qingyu Wang, Jiaojiao Nie,

Zejinxuan Liu

et al.

Nanoscale Advances, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

HA2-16 ferritin nanoparticles induce stem-directed neutralizing antibodies by H3 influenza virus boost.

Language: Английский

Citations

0
A novel pan-epitope based nanovaccine self-assembled with CpG enhances immune responses against flavivirus DOI Creative Commons

Jiuxiang He,

Xiaoyan Ding, Jing Zhao

et al.

Journal of Nanobiotechnology, Journal Year: 2024, Volume and Issue: 22(1)

Published: Nov. 28, 2024

Flavivirus is a highly prevalent and outbreak-prone disease, affecting billions of individuals annually posing substantial public health challenges. Vaccination critical to reducing the global impact flavivirus infections, making development safe effective vaccine top priority. The self-assembled pan-epitope presents key advantages for improving immunogenicity safety without relying on external vectors or adding immunomodulatory elements, both which are essential successful development. In this study, peptide TBT was combined with adjuvant CpG form TBT-CpG nanovaccine (TBT-CpG NaVs), found be spherical, uniform in shape, demonstrated strong serum stability. vitro studies showed that NaVs were efficiently taken up internalized by bone marrow-derived dendritic cells (BMDCs). Flow cytometry transcriptomic analysis indicated antigens effectively presented antigen-presenting (APCs) via MHC II pathway, facilitated BMDCs maturation promoted release pro-inflammatory cytokines IL-1β, TNF-α, IL-6. vivo confirmed enhanced antigen-specific IgG levels, significantly increased IFN-γ IL-4 expression spleen cells, offered protective effects against Dengue virus (DENV) Zika (ZIKV) infections. Safety evaluations revealed no hepatotoxicity significant organ damage immunized mice. candidate activates triggers targeted immune response, providing antiviral DENV ZIKV. This demonstrates good safety, establishing promising foundation new strategy vaccines.

Language: Английский

Citations

3
Self‐Assembled Multiepitope Nanovaccine Provides Long‐Lasting Cross‐Protection against Influenza Virus DOI Open Access
Jiaojiao Nie, Qingyu Wang, Chenxi Li

et al.

Advanced Healthcare Materials, Journal Year: 2023, Volume and Issue: 13(10)

Published: Nov. 21, 2023

Abstract Seasonal influenza vaccines typically provide strain‐specific protection and are reformulated annually, which is a complex time‐consuming process. Multiepitope vaccines, combining multiple conserved antigenic epitopes from pathogen, can trigger more robust, diverse, effective immune responses, providing potential solution. However, their practical application hindered by low immunogenicity short‐term effectiveness. In this study, linear the stem domain of hemagglutinin ectodomain matrix protein 2 combined with Helicobacter pylori ferritin, stable self‐assembled nanoplatform, to develop an multiepitope nanovaccine, named MHF. MHF prokaryotically expressed in soluble form self‐assembles into uniform nanoparticles. The subcutaneous immunization mice adjuvanted induces cross‐reactive neutralizing antibodies, antibody‐dependent cell‐mediated cytotoxicity, cellular immunity, offering complete against H3N2 as well partial H1N1. Importantly, vaccine cargo delivered ferritin triggers epitope‐specific memory B‐cell antibody level persisting for over 6 months post‐immunization. These findings indicate that nanovaccines elicit potent long‐lasting responses while significantly reducing risk escape mutants, offer greater practicality terms scalable manufacturing genetic manipulability, presenting promising strategy future development.

Language: Английский

Citations

7
Multi-epitope vaccine design for hepatitis E virus based on protein ORF2 and ORF3 DOI Creative Commons
Qiong Lu, Hao Wu,

Jing Meng

et al.

Frontiers in Microbiology, Journal Year: 2024, Volume and Issue: 15

Published: March 21, 2024

Introduction Hepatitis E virus (HEV), with heightened virulence in immunocompromised individuals and pregnant women, is a pervasive threat developing countries. A globaly available vaccine against HEV currently lacking. Methods We designed multi-epitope based on protein ORF2 ORF3 of using immunoinformatics. Results The comprised 23 nontoxic, nonallergenic, soluble peptides. stability the docked peptide vaccine-TLR3 complex was validated by molecular dynamic simulations. induction effective cellular humoral immune responses multi-peptide verified simulated immunization. Discussion These findings provide foundation for future studies.

Language: Английский

Citations

2
A photoelectrochemical immunosensor based on cobalt corrole sensitized carbon nitride for human norovirus detection DOI
Junchao Qian, Yun Chen, Guifen Lu

et al.

Sensors and Actuators B Chemical, Journal Year: 2024, Volume and Issue: 418, P. 136348 - 136348

Published: July 26, 2024

Language: Английский

Citations

1
VP8 Mosaic Nanoparticles Elicit Cross-Neutralizing Immune Responses and Provide Protection Against Heterotypic Rotavirus Challenge in Mice DOI
Feibo Song, Yuanjun Zeng, Roufang Sheng

et al.

ACS Nano, Journal Year: 2024, Volume and Issue: 18(46), P. 31809 - 31822

Published: Nov. 5, 2024

Group A rotaviruses (RVA) remain one of the dominant pathogens causing diarrhea in children under 5 years age worldwide, despite a sharp decrease RVA-associated and mortality since introduction rotavirus vaccines. The decreased effectiveness live attenuated vaccines, coupled with emergence new genotypes risk cross-species virus transmission, underscores necessity to develop more effective broad-spectrum In this study, we utilized nanoparticles SpyCatcher-SpyTag system effectively display truncated VP8-1 protein. modular monovalent proteins markedly increased immunogenicity VP8-1. Furthermore, bivalent from simian SA11 lamb LLR on same particle not only against homotypic antigens but also elicited robust heterotypic immune responses conferred protection distant sequence identities 62%-66% an adult mouse model. Therefore, mosaic VP8 could be considered as viable strategy for development next-generation vaccine.

Language: Английский

Citations

1
The emergence of nanovaccines as a new paradigm in virological vaccinology: a review DOI Creative Commons
Chittaranjan Baruah, Pankaj Das, Papari Devi

et al.

Exploration of Immunology, Journal Year: 2023, Volume and Issue: unknown, P. 361 - 383

Published: Aug. 31, 2023

Vaccination has made an enormous contribution to global health. Treatment resistance for infectious diseases is growing quickly, and chemotherapeutic toxicity in cancer means that vaccines must be right away save humanity. But subunit vaccinations alone don’t give enough strong long-lasting protection against infections can kill. Nanoparticle (NP)-based delivery vehicles, such as dendrimers, liposomes, micelles, virosomes, nanogels, microemulsions, offer interesting ways get around the problems with traditional vaccine adjuvants. The nanovaccines (50–250 nm size) are most efficient terms of tissue targeting, staying bloodstream a long time. Nanovaccines improve antigen presentation, targeted delivery, stimulation body’s innate immune system, T-cell response without putting people at risk. This help fight cancers. Also, very helpful making treatments use immunotherapy. So, this review highlights various types NPs used techniques have worked new paradigm viral vaccinology diseases. It gives full rundown current NP-based vaccines, their potential adjuvants, they delivered cells. In future, best will try more logically designed, antigens them, fully functionalized, given people.

Language: Английский

Citations

2