Microorganisms,
Journal Year:
2023,
Volume and Issue:
11(7), P. 1768 - 1768
Published: July 6, 2023
Lysobacter
species
have
attracted
increasing
attention
in
recent
years
due
to
their
capacities
produce
diverse
secondary
metabolites
against
phytopathogens.
In
this
research,
we
analyzed
the
genomic
and
transcriptomic
patterns
of
capsici
CK09.
Our
data
showed
that
L.
CK09
harbored
various
contact-independent
biocontrol
traits,
such
as
fungal
cell
wall
lytic
enzymes
HSAF/WAP-8294A2
biosynthesis,
well
several
contact-dependent
machineries,
including
type
2/4/6
secretion
systems.
Additionally,
a
variety
hydrolytic
enzymes,
particularly
extracellular
were
found
genome
predicted
improve
its
adaption
soil.
Furthermore,
systems,
4
pili,
3
system
polysaccharide
can
provide
selective
advantage
CK09,
enabling
live
on
surface
The
expression
these
genes
was
then
confirmed
via
analysis,
indicating
activities
genes.
Collectively,
our
research
provides
comprehensive
understanding
potential
soil
implies
strain
for
application
future.
Frontiers in Microbiology,
Journal Year:
2024,
Volume and Issue:
15
Published: June 20, 2024
Introduction
Anthracnose
is
a
significant
fungal
disease
that
affects
tree
growth
and
development,
with
Colletotrichum
spp.
exhibiting
host
non-specificity
targeting
various
organs,
making
control
challenging.
Methods
This
study
aimed
to
identify
the
pathogenic
species
causing
anthracnose
in
Ilex
macrocarpa
Nanchong,
Sichuan
Province,
screen
effective
fungicides,
particularly
biological
ones.
The
pathogen
was
identified
as
fioriniae
through
morphological
observation,
pathogenicity
assays,
molecular
methods.
Three
five
chemical
fungicides
were
evaluated
for
their
effects
on
mycelial
spore
germination
rate
of
pathogen.
Results
results
indicated
prochloraz
most
fungicide,
while
cell-free
supernatant
(CFS)
Bacillus
velezensis
had
inhibitory
effect
among
fungicides.
Transcriptome
analysis
revealed
CFS
B.
significantly
reduced
expression
genes
associated
ribosomes,
genetic
information
processing,
membrane
lipid
metabolism,
sphingolipid
biosynthesis
C.
.
Additionally,
glutathione
pathway’s
genes,
including
key
such
GST,
GFA,
Grx,
TRR,
POD,
induced.
Furthermore,
17
MFS
transporters
9
ABC
increased.
Autophagy-related
ATGs
also
affected
by
CFS.
Discussion
These
findings
suggest
may
inhibit
interference
cell
energy
metabolism.
provide
potential
target
insights
into
antifungal
mechanism
which
inhibits
AgriEngineering,
Journal Year:
2024,
Volume and Issue:
6(3), P. 2366 - 2384
Published: July 22, 2024
Trichoderma
is
one
of
the
most
widely
used
microorganisms
in
biological
control
plant
pathogens.
The
techniques
for
its
formulation
are
well
known
and
commercially
distributed
both
solid
liquid
presentations
based
on
formulations
reproductive
structures.
Currently,
agricultural
systems
integrate
this
type
fungus
as
an
alternative
sustainable
production,
even
though
traditional
still
has
important
limitations,
it
a
high
potential
to
be
combined
with
new
technologies
development
innovation
products
that
improve
their
effectiveness.
In
response
this,
micro-
nanotechnology
presented
alternatives
technify
bioagents,
promoting
greater
resistance,
viability,
dissemination
biomass
metabolites
through
encapsulation
smart
delivery
techniques.
Some
works
have
been
developed
achieve
especially
using
ionic
gelation,
good
results
agriculture.
work,
some
generalities
organism
mentioned,
including
common
applications,
information
related
systems,
improvement
biologics
represented
by
microencapsulation.
Plants,
Journal Year:
2024,
Volume and Issue:
13(16), P. 2180 - 2180
Published: Aug. 6, 2024
Fusarium
root
rot
caused
by
the
species
complex
significantly
affects
yield
and
quality
of
Angelica
sinensis,
a
valuable
medicinal
herb.
Traditional
management
primarily
relies
on
chemical
fungicides,
which
have
led
to
pathogen
resistance,
environmental
hazards,
concerns
regarding
public
health
active
components
in
A.
sinensis.
This
study
explores
efficacy
novel
plant-derived
biopesticide
Shi
Chuang
Zhi
Feng
Ning
(T1;
SCZFN),
alongside
Bacillus
subtilis
wettable
powder
(T2)
fungicide
(T3),
controlling
understanding
their
impacts
rhizosphere
microbial
community
metabolome.
Results
field
experiment
demonstrated
that
treatments
T1
T3
achieved
control
efficiencies
73.17%
75.45%,
respectively,
outperforming
T2
(39.99%)
control.
High-throughput
sequencing
revealed
all
altered
diversity
structure
communities,
with
reducing
abundance
taxa
linked
rot,
such
as
Muribaculaceae
spp.,
Humicola
Mycochlamys
spp.
Treatment
notably
enhanced
beneficial
bacterial
taxa,
including
Acidobacteria
Nitrospira
Pedosphaeraceae
involved
carbon
cycling
plant
growth
promotion.
Metabolomic
analysis
identified
39,
105,
45
differentially
expressed
metabolites
(DEMs)
across
treatments,
demonstrating
T1's
potential
modulate
metabolome
effectively.
Further,
correlation
stronger
between
distinct
microorganisms
significant
influence
DEMs
treatment
compared
other
treatments.
These
findings
underscore
SCZFN's
role
enhancing
disease
suppression
providing
insights
into
its
biocontrol
mechanisms
supporting
development
sustainable
strategies
cultivation.
Microorganisms,
Journal Year:
2023,
Volume and Issue:
11(11), P. 2735 - 2735
Published: Nov. 9, 2023
Plant
pathogenic
bacteria
pose
a
significant
threat
to
olive
cultivation,
leading
substantial
economic
losses
and
reduced
yield.
The
efficacy
of
antimicrobial
agents
against
these
pathogens
is
great
interest
for
sustainable
disease
management
strategies.
As
such,
the
knot
one
major
challenges
in
protection.
In
presented
study,
through
series
vitro
assays,
we
investigated
effect
six
essential
oils
(EOs)
their
most
concentrated
constituents
causative
agent
disease-Pseudomonas
savastanoi
pv.
savastanoi,
highlighting
high
potential
Origanum
compactum
EO
its
constituent
carvacrol.
Carvacrol
exhibited
highest
practical
application,
demonstrating
membrane
disruption
as
mechanism
action
even
at
lowest
concentration.
bactericidal
antimicrobials
was
confirmed
time-kill
assay,
where
concentrations
MIC,
2×
4×
MIC
were
evaluated.
Some
applied
treatments
resulted
inhibition
equal
or
higher
than
copper-based
treatment.
Additionally,
assessed
phytotoxicity
carvacrol
by
foliar
application
on
cv.
Leccino.
appearance
phytotoxic
injuries
majorly
occurred
young
leaves
plants,
with
proportion
damaged
canopy
observed
when
concentration
applied.
Due
efficiency
P.
vitro,
findings
highlight
molecule
development
environmentally
friendly
biopesticides.
This
study
also
contributes
advancement
practices
enhanced
crop
Frontiers in Plant Science,
Journal Year:
2023,
Volume and Issue:
14
Published: Dec. 18, 2023
Background
Angular
leaf
spot
disease
caused
by
plant
pathogenic
bacterium
Xanthomonas
fragariae
seriously
threatens
strawberry
crop
production
globally.
Methods
In
this
study,
we
sequenced
the
whole
genome
of
X.
YM2,
isolated
from
Yunnan
Province,
China.
addition,
performed
a
comparative
analysis
YM2
with
two
existing
strains
YL19
and
SHQP01
Liaoning
Shanghai,
respectively.
Results
The
results
Nanopore
sequencing
showed
that
comprises
one
single
chromosome
contig
size
4,263,697
bp,
plasmid
0.39
Mb,
GC
content
ratio
62.27%,
3,958
predicted
coding
genes.
gum
,
hrp
rpf
xps
gene
clusters
lipopolysaccharide
(LPS),
which
are
typical
virulence
factors
in
species.
By
performing
genomic
between
YL19,
SHQP01,
found
strain
is
similar
to
regarding
contents.
However,
there
minor
differences
composition
major
homologous
clusters.
Furthermore,
collinearity
demonstrated
has
lower
similarity
longer
evolutionary
distance
but
more
closely
related
SHQP01.
Conclusions
availability
high-quality
genetic
resource
will
serve
as
basic
tool
for
investigating
biology,
molecular
pathogenesis,
YM2.
unraveling
potential
vulnerabilities
its
makeup
aid
developing
effective
suppression
control
measures.
Metabolites,
Journal Year:
2024,
Volume and Issue:
14(9), P. 507 - 507
Published: Sept. 20, 2024
[Background]
Intercropping
is
considered
an
effective
approach
to
defending
rice
disease.
[Objectives/Methods]
This
study
aimed
explore
the
resistance
mechanism
of
intraspecific
intercropping
by
investigating
soil
metabolites
and
their
regulation
on
rhizosphere
microbial
community
using
metabolomic
microbiome
analyses.
[Results]
The
results
showed
that
panicle
blast
disease
occurrence
resistant
variety
Shanyou63
(SY63)
susceptible
Huangkenuo
(HKN)
were
both
decreased
in
compared
monoculture.
Notably,
HKN
system
exhibited
significantly
incidence
increased
resistance-related
enzyme
protease
activity.
KEGG
annotation
from
metabolomics
analysis
revealed
phenylalanine
metabolic
pathway,
phenylalanine,
tyrosine,
tryptophan
biosynthesis
fructose
mannose
pathway
key
pathways
related
resistance.
Soil
indicated
bacterial
genera
Nocardioides,
Marmoricola,
Luedemannella,
Desulfomonile
enriched
after
intercropping,
while
SY63
experienced
a
substantial
accumulation
Ruminiclostridium
Cellulomonas.
Omics-based
correlation
highlighted
assembly
Cellulomonas
affected
content
D-sorbitol,
D-mannitol,
quinic
acid,
which
further
proved
acid
had
inhibitory
effect
mycelium
growth
Magnaporthe
oryzae,
these
three
significant
control
effect.
optimal
blast-control
efficiency
was
51.72%,
Lijiangxintuanheigu
(LTH)
64.57%.
[Conclusions]
These
findings
provide
theoretical
basis
for
varieties
sustainable
production,
emphasizing
novelty
elucidating
underlying
mechanisms
intercropping-mediated
Microorganisms,
Journal Year:
2023,
Volume and Issue:
11(7), P. 1768 - 1768
Published: July 6, 2023
Lysobacter
species
have
attracted
increasing
attention
in
recent
years
due
to
their
capacities
produce
diverse
secondary
metabolites
against
phytopathogens.
In
this
research,
we
analyzed
the
genomic
and
transcriptomic
patterns
of
capsici
CK09.
Our
data
showed
that
L.
CK09
harbored
various
contact-independent
biocontrol
traits,
such
as
fungal
cell
wall
lytic
enzymes
HSAF/WAP-8294A2
biosynthesis,
well
several
contact-dependent
machineries,
including
type
2/4/6
secretion
systems.
Additionally,
a
variety
hydrolytic
enzymes,
particularly
extracellular
were
found
genome
predicted
improve
its
adaption
soil.
Furthermore,
systems,
4
pili,
3
system
polysaccharide
can
provide
selective
advantage
CK09,
enabling
live
on
surface
The
expression
these
genes
was
then
confirmed
via
analysis,
indicating
activities
genes.
Collectively,
our
research
provides
comprehensive
understanding
potential
soil
implies
strain
for
application
future.
