About the Mechanism of Light-Induced Age-Related Decrease of Melanin Concentration in the Retinal Pigment Epithelium Cells

Published: July 27, 2023

It is known that during the process of aging there a significant decrease in number melanosomes retinal pigment epithelium (RPE) cells human eye. We have previously shown interaction melanin with superoxide radicals results its oxidative degradation formation water-soluble fluorescent products. In present study, we show using fluorescence analysis, HPLC, and mass spectrometry, visible light irradiation melanolipofuscin granules isolated from RPE eye products melanin, contrast to lipofuscin irradiation. The these occurs as result by radicals, which are generated part granule. identified both composition irradiated light, non-irradiated but oxidized melanosomes. ions could be associated were applying principal component analysis time-of-flight secondary ion spectrometry (ToF-SIMS) data. Degradation intact also possible; however, this requires significantly higher intensities than for granules. concluded concentration age due reactive oxygen species under action light.

Language: Английский

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Activation of aryl hydrocarbon receptor by azatyrosine-phenylbutyric hydroxamide inhibits progression of diabetic retinopathy mice

Biochemical Pharmacology, Journal Year: 2023, Volume and Issue: 215, P. 115700 - 115700

Published: July 22, 2023

Language: Английский

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Short-Term and Long-Term Effects after Exposure to Ionizing Radiation and Visible Light on Retina and Retinal Pigment Epithelium of Mouse Eye

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(23), P. 17049 - 17049

Published: Dec. 1, 2023

A comparative in vivo study of the effects ionizing radiation (accelerated protons) and visible light (400-700 nm) on retina retinal pigment epithelium (RPE) mouse eye was carried out. Using methods fluorescence spectroscopy high-performance liquid chromatography (HPLC), we analyzed relative composition retinoids chloroform extracts obtained from retinas RPEs immediately after exposure animals to various types 4.5 months they were exposed maintained under standard conditions throughout period. The fluorescent properties shown change upon radiation. This fact indicates accumulation retinoid oxidation degradation products RPE. data HPLC analyses indicate that when radiation, processes similar photooxidation occur. Both high-intensity have been be characterized by long-term effects. action any type is assumed activate mechanism enhanced reactive oxygen species production, resulting a damaging effect.

Language: Английский

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Mitochondrial damage and clearance in retinal pigment epithelial cells

Acta Ophthalmologica, Journal Year: 2024, Volume and Issue: 102(S282), P. 3 - 53

Published: March 1, 2024

Abstract Age‐related macular degeneration (AMD) is a devastating eye disease that causes permanent vision loss in the central part of retina, known as macula. Patients with such severe visual face reduced quality life and are at 1.5 times greater risk death compared to general population. Currently, there no cure for or effective treatment dry AMD. There several mechanisms thought underlie disease, example, ageing‐associated chronic oxidative stress, mitochondrial damage, harmful protein aggregation inflammation. As way gaining better understanding molecular behind AMD thus developing new therapies, we have created peroxisome proliferator‐activated receptor gamma coactivator 1‐alpha nuclear factor erythroid 2‐related 2 ( PGC1α / NFE2L2 ) double‐knockout (dKO) mouse model mimics many clinical features AMD, including elevated levels stress markers, damaged mitochondria, accumulating lysosomal lipofuscin extracellular drusen‐like structures retinal pigment epithelial cells (RPE). In addition, human RPE cell‐based was established examine impact non‐functional intracellular clearance systems on inflammasome activation. this study, found disturbance autolysosomal machinery responsible clearing mitochondria one‐year‐old PGC1α/NFE2L2‐deficient mice. The confocal immunohistochemical analysis revealed an increase autophagosome marker microtubule‐associated proteins 1A/1B light chain 3B (LC3B) well multiple mitophagy markers PTE‐induced putative kinase 1 (PINK1) E3 ubiquitin ligase (PARKIN), along signs mitochondria. However, autolysosome formation detected, nor colocalization LAMP2 marker, ATP synthase β. upregulation late fusion Ras‐related (Rab7) perinuclear space cells, together autofluorescent aggregates. Additionally, observed numbers Toll‐like receptors 3 9, while those NOD‐like were decreased PGC1α/NFE2L2 dKO specimens wild‐type animals. trend towards increased complement component C5a involvement serine protease enzyme, thrombin, enhancing terminal pathway producing C5a, independent C3. primary acute phase C‐reactive advanced glycation end products also retina. Furthermore, selective proteasome inhibition epoxomicin promoted both nicotinamide adenine dinucleotide phosphate (NADPH) oxidase mitochondrial‐mediated leading release DNA cytosol, resulting potassium efflux‐dependent activation absent melanoma (AIM2) subsequent secretion interleukin‐1β ARPE‐19 cells. conclusion, data suggest least relative decrease mitophagy, increases amounts C5 thrombin C3 AMD‐like model. Moreover, evoked damage AIM2

Language: Английский

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Electronic Visual Prostheses

Neuroscience and Behavioral Physiology, Journal Year: 2024, Volume and Issue: 54(2), P. 293 - 300

Published: Feb. 1, 2024

Language: Английский

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The Inhibitory Effect of Oxibiol on the Process of Protein Modification by Water-Soluble Products of Photo-Oxidative Destruction of Bisretinoid A2E

BIOPHYSICS, Journal Year: 2024, Volume and Issue: 69(2), P. 219 - 224

Published: April 1, 2024

Language: Английский

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A new approach to assessing the consequences of radiation on the eye

Вестник Российской академии наук, Journal Year: 2024, Volume and Issue: 94(7), P. 658 - 664

Published: Sept. 29, 2024

The authors propose a new approach to assessing the consequences of exposure ionizing radiation on structures eye. is based results recently obtained by together with employees Joint Institute for Nuclear Research in Dubna, according which causes oxidation bisretinoids contained eye - retina and retinal pigment epithelium. As result this oxidation, fluorescence spectrum shifts blue region visible spectrum. shift can be recorded non-invasively using method recording fundus autofluorescence, currently generally accepted ophthalmology. Since occurs during exposure, it becomes possible almost immediately after irradiation assess degree impact both body as whole. There no analogue such non-invasive assessment effects body. proposed may become important safety nuclear industry workers, astronauts, patients undergoing proton or gamma therapy.

Language: Английский

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Biophysical Reviews: Turning the page from 2022 to 2023

Biophysical Reviews, Journal Year: 2023, Volume and Issue: 15(1), P. 1 - 11

Published: Feb. 1, 2023

Language: Английский

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Editorial for the special issue of biophysical reviews on the 9th Congress of the Russian society for photobiology held in Shepsi, Krasnodar region, Russia, on September 12–19, 2021

Biophysical Reviews, Journal Year: 2022, Volume and Issue: 14(4), P. 743 - 749

Published: Aug. 1, 2022

Language: Английский

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Retinal electronic prostheses

Сенсорные системы, Journal Year: 2023, Volume and Issue: 37(3), P. 205 - 217

Published: July 1, 2023

Language: Английский

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