Wastewater-based epidemiology framework: Collaborative modeling for sustainable disease surveillance

The Science of The Total Environment, Journal Year: 2025, Volume and Issue: 968, P. 178889 - 178889

Published: Feb. 19, 2025

Language: Английский

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Wastewater-Based Epidemiology for Viral Surveillance from an Endemic Perspective: Evidence and Challenges

Viruses, Journal Year: 2024, Volume and Issue: 16(3), P. 482 - 482

Published: March 20, 2024

Wastewater-based epidemiology (WBE) is currently used to monitor not only the spread of viral SARS-CoV-2 pandemic but also that other viruses in endemic conditions, particularly absence syndromic surveillance. The continuous monitoring sewage requires high expenditure and significant time investments, highlighting need for standardized methods structured strategies. In this context, we conducted weekly wastewater northwestern Tuscany (Italy) targeted human adenovirus (HAdV), norovirus genogroup II (NoVggII), enterovirus (EV), SARS-CoV-2. Samples were collected at entrances treatment plants concentrated using PEG/NaCl precipitation, nucleic acids extracted detected through real-time reverse transcription qPCR. NoVggII was most identified target (84.4%), followed by HAdV, SARS-CoV-2, EV. Only HAdV EV exhibited seasonal peaks spring summer. Compared with data previously same study area (from February 2021 September 2021), results revealed a shift from an epidemic pattern, least region under investigation, which likely due mutations led spreading new variants increased resistance summer environmental conditions. conclusion, efficient strategy, WBE proves valuable surveillance scenarios, enabling identification temporal–local distribution patterns are useful making informed public health decisions.

Language: Английский

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Environmental Dissemination of SARS-CoV-2: An Analysis Employing Crassphage and Next-Generation Sequencing Protocols

Food and Environmental Virology, Journal Year: 2025, Volume and Issue: 17(1)

Published: Jan. 7, 2025

Language: Английский

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Variability of Clinical Metrics in Small Population Communities Drive Perceived Wastewater and Environmental Surveillance Data Quality: Ontario, Canada-Wide Study

ACS ES&T Water, Journal Year: 2025, Volume and Issue: 5(4), P. 1605 - 1619

Published: March 7, 2025

The emergence of COVID-19 in Canada has led to over 4.9 million cases and 59,000 deaths by May 2024. Traditional clinical surveillance metrics (hospital admissions laboratory-positive cases) were complemented with wastewater environmental monitoring (WEM) monitor SARS-CoV-2 incidence. However, challenges public health integration WEM persist due perceived limitations data quality, potentially driving inconsistent correlations variability lead times. This study investigates how factors like population size, measurement magnitude, site isolation status, hospital admissions, affect Ontario. analysis uncovers a direct relationship between the size surveyed sewersheds, while magnitude was not directly impacted size. Higher observed smaller likely reducing correlation strength for inferring Population significantly influenced thresholds identified at ∼66,000 inhabitants strong WEM-hospital ∼68,000 WEM-laboratory-positive during waned vaccination periods Ontario (the Omicron BA.1 wave). During significant immunization BA.2 wave), these increased ∼187,000 238,000, respectively. These findings highlight benefit strategic interventions, especially communities. provides insights enhancing decision making disease through WEM, applicable other diseases.

Language: Английский

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Wastewater Based Epidemiology for Viral Surveillance in an Endemic Perspective: Evidence and Challenges

Published: Feb. 8, 2024

Wastewater-Based Epidemiology (WBE) is currently used to monitor not only the spread of pandemic virus SARS-CoV-2 but also for other viruses in endemic conditions, particularly absence syndromic surveillance. Continuous monitoring sewage requires high costs and significant time investment, highlighting need standardized methods structured strategies. In this context, we conducted weekly wastewater northwestern Italy, targeting Human Adenovirus (HAdV), Norovirus Genogroup II (NoVggII), Enterovirus (EV), SARS-CoV-2. Samples collected at entrance treatment plants were concentrated using PEG/NaCl precipitation viral nucleic acids extracted detected by real-time (RT-)qPCR. NoVggII resulted most identified target (84.4%), followed HAdV, SARS-CoV-2, EV. Only HAdV EV exhibited seasonal peaks spring summer. results compared with data previously same study area (February 2021 September 2021) revealed a shift from an epidemic status, likely due evolution variants. conclusion, WBE, efficient strategy, proves valuable surveillance scenarios, enabling identification temporal-local distribution patterns that are useful making informed public health decision.

Language: Английский

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Wastewater Treatment Plants Performance for Reuse: Evaluation of Bacterial and Viral Risks

Water, Journal Year: 2024, Volume and Issue: 16(10), P. 1399 - 1399

Published: May 14, 2024

Reusing reclaimed water is of paramount importance to achieve the 2030 Agenda for Sustainable Development Goals 6 and 13. In Europe, a recent Regulation set minimum requirements reuse in agriculture. However, some challenges remain considering microbial risks their prevention. this study, two urban wastewater treatment plants (WWTPs) were investigated from perspective reuse. A five-year investigation was performed on routine monitoring parameters collected under different weather conditions (wet/dry) treatments (chlorination/non-chlorination) inlet outlet samples. Moreover, three-month focused parameters, including indicators, index pathogens (Human Adenovirus—HAdV, Salmonella spp.), other viral (norovirus, enterovirus, SARS-CoV-2). The long-term study revealed compliance both WWTPs chemical (organic substances solids) more than 90% samples, whereas Escherichia coli, ranged 96.1% with chlorination dry 16.7% without wet days. E. coli positively associated oxygen demand (COD), which could be promising online measurable proxy coli. performance demonstrated sound reliability detecting as suitable surrogate chlorinated effluents, but neither bacterial nor indicators are able represent HAdV. Although remove most considered, seems insufficient safety.

Language: Английский

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Wastewater-based surveillance of respiratory viruses in Northern Tuscany (Italy): Challenges and added value for public health purposes

The Science of The Total Environment, Journal Year: 2024, Volume and Issue: 957, P. 177752 - 177752

Published: Nov. 30, 2024

During the COVID-19 pandemic, wastewater-based surveillance (WBS) showed great potential as an early warning system and could complement human clinical surveillance. This study aimed to highlight added value of WBS for respiratory infections alongside different systems. Sewage collected at entry four Wastewater Treatment Plants in Northern Tuscany (Italy) were analyzed SARS-CoV-2, Human Adenovirus (HAdV), Respiratory Syncytial Virus (RSV) Influenza (IV), over two years. Clinical data available area, while other viruses came from national virological For correlation was highly significant between hospitalization (ρ = 0.8460), but not wastewater or 0.1682 ρ 0.0569, respectively). SARS-CoV-2 RNA found even period when cases reported, indicating a continuous community circulation. HAdVs detected 74.3 % samples, most sequences identified belonged enteric species (HAdV-F41), need distinguishing causing diseases RSV only winter 2022-2023, IV had been wastewater, probably due poor test sensitivity. In conclusion, although there may be various challenges testing targets, can provide pathogen-specific situational assessment which complements existing

Language: Английский

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Leveraging Wastewater: Validating the National-Scale SARS-CoV-2 Surveillance System in Cyprus for Elevated Public Health Surveillance and Enhanced Epidemiological Insight

Journal of environmental chemical engineering, Journal Year: 2024, Volume and Issue: 12(3), P. 112953 - 112953

Published: May 6, 2024

Language: Английский

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